Restorative effect of kalpaamruthaa, an indigenous preparation, on oxidative damage in mammary gland mitochondrial fraction in experimental mammary carcinoma

Arul Kumaran Shanmugam, Vanu Ramkumar Ramprasath, Palanivelu Shanthi, Panchanatham Sachdanandam

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Cancer prevention and treatment using phytochemicals have attracted increased interest. Recent studies have shown that Semecarpus anacardium Linn nut milk extract (SA), a promising antioxidant and anticancer drug, exerts its anticancer effect through reducing or quenching reactive oxygen species under different conditions. The present study examined whether Phyllanthus emblica Linn fruit, rich in vitamin C content synergistically in combination can enhance both the antioxidant and anticancer activity of S. anacardium nut milk extract in 7, 12-dimethyl benz[a]anthracene (DMBA)-induced experimental mammary carcinoma in rat model. Female Sprague Dawley rats of 180 ± 10g were categorized into six groups. Three groups were administered DMBA (25mg/rat, orally) dissolved in olive oil to induce mammary carcinoma. One of these groups received Kalpaamruthaa (KA) (300mg/kg b.wt, orally) and other group received SA (200mg/kg b.wt, orally) for 14 days after 90 days of DMBA induction. A vehicle treated control and drug control groups were also included. The mitochondrial fraction of untreated DMBA-induced mammary gland showed 2.61-fold increase in lipid peroxidation level and abnormal changes in the activities/levels of mitochondrial enzymic (superoxide dismutase, glutathione peroxidase and glutathione reductase) and non-enzymic (glutathione, vitamin C and vitamin E) antioxidants were observed. DMBA treated rats also showed decline in the activities of mitochondrial enzymes such as succinate dehydrogenase, malate dehydrogenase, α-ketoglutarate dehydrogenase and isocitrate dehydrogenase. In contrast, rats treated with Kalpaamruthaa showed normal lipid peroxide level and antioxidant defenses. The results of the present study highlight the improved antioxidant property of KA than sole treatment of S. anacardium nut milk extract.

Original languageEnglish (US)
Pages (from-to)77-82
Number of pages6
JournalMolecular and Cellular Biochemistry
Volume291
Issue number1-2
DOIs
StatePublished - Oct 1 2006

Fingerprint

Human Mammary Glands
Semecarpus
Rats
Antioxidants
Nuts
Breast Neoplasms
Milk
Ascorbic Acid
Phyllanthus emblica
Isocitrate Dehydrogenase
9,10-Dimethyl-1,2-benzanthracene
Malate Dehydrogenase
Succinate Dehydrogenase
Glutathione Reductase
Lipid Peroxides
Drug and Narcotic Control
Phytochemicals
Glutathione Peroxidase
Fruits
Vitamin E

Keywords

  • Kalpaamruthaa
  • Lipid peroxidation
  • Mitochondrial enzymes
  • Phyllanthus emblica
  • Semecarpus anacardium

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Restorative effect of kalpaamruthaa, an indigenous preparation, on oxidative damage in mammary gland mitochondrial fraction in experimental mammary carcinoma. / Shanmugam, Arul Kumaran; Ramprasath, Vanu Ramkumar; Shanthi, Palanivelu; Sachdanandam, Panchanatham.

In: Molecular and Cellular Biochemistry, Vol. 291, No. 1-2, 01.10.2006, p. 77-82.

Research output: Contribution to journalArticle

Shanmugam, Arul Kumaran ; Ramprasath, Vanu Ramkumar ; Shanthi, Palanivelu ; Sachdanandam, Panchanatham. / Restorative effect of kalpaamruthaa, an indigenous preparation, on oxidative damage in mammary gland mitochondrial fraction in experimental mammary carcinoma. In: Molecular and Cellular Biochemistry. 2006 ; Vol. 291, No. 1-2. pp. 77-82.
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