Reticulon 3 mediates Bcl-2 accumulation in mitochondria in response to endoplasmic reticulum stress

Qingwen Wan, Ersheng Kuang, Wei Dong, Shumin Zhou, Hua Xu, Yipeng Qi, Yingle Liu

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Reticulon3 (RTN3), firstly isolated from the retina and widely expressed in human tissues with the highest expression in the brain, is presumed to play an important role in the developing axons through the transport of liquids and proteins. We have identified and characterized RTN3 as a RTN4B/ASY interaction protein. Here we demonstrated that ER-stress activated RTN3 expression. CHOP and ATF6 were sufficient to up-regulate the expression of RTN3. The down-regulation of RTN3 would induce apoptosis and attenuate the anti-apoptotic activity of Bcl-2, indicating RTN3 was required for the cellular survival and optimal anti-apoptotic activity of Bcl-2. Our present studies also indicated ER-stress induced RTN3 up-regulation could trigger Bcl-2 translocation from ER to mitochondria. Moreover, the previous studies showed that RTN4B was also a Bcl-2-interacted protein. We found that RTN3 and RTN4B could block the access of Bcl-2 to each other and thereafter determined the Bcl-2 subcellular distribution. Taken together, our findings indicate that RTN3 is directly involved in the ER-constituents trafficking events through dually acting as an essential and important ER-stress sensor, and a trigger for the Bcl-2 translocation.

Original languageEnglish (US)
Pages (from-to)319-328
Number of pages10
JournalApoptosis
Volume12
Issue number2
DOIs
StatePublished - Feb 1 2007

Fingerprint

Mitochondria
Endoplasmic Reticulum Stress
Up-Regulation
Axons
Retina
Carrier Proteins
Proteins
Down-Regulation
Apoptosis
Survival
Brain
Tissue
Sensors
Liquids

Keywords

  • Bcl-2
  • Endoplasmic reticulum stress
  • Mitochondria
  • RTN3

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science
  • Clinical Biochemistry
  • Cell Biology
  • Biochemistry, medical
  • Cancer Research

Cite this

Reticulon 3 mediates Bcl-2 accumulation in mitochondria in response to endoplasmic reticulum stress. / Wan, Qingwen; Kuang, Ersheng; Dong, Wei; Zhou, Shumin; Xu, Hua; Qi, Yipeng; Liu, Yingle.

In: Apoptosis, Vol. 12, No. 2, 01.02.2007, p. 319-328.

Research output: Contribution to journalArticle

Wan, Qingwen ; Kuang, Ersheng ; Dong, Wei ; Zhou, Shumin ; Xu, Hua ; Qi, Yipeng ; Liu, Yingle. / Reticulon 3 mediates Bcl-2 accumulation in mitochondria in response to endoplasmic reticulum stress. In: Apoptosis. 2007 ; Vol. 12, No. 2. pp. 319-328.
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