Retrograde regulation of motoneuron differentiation by muscle β-catenin

Xiao Ming Li, Xian Ping Dong, Shi Wen Luo, Bin Zhang, Dae Hoon Lee, Annie K.L. Ting, Hannah Neiswender, Chang Hoon Kim, Ezekiel Carpenter-Hyland, Tian Ming Gao, Wencheng Xiong, Lin Mei

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Abstract

Synapse formation requires proper interaction between pre- and postsynaptic cells. In anterograde signaling, neurons release factors to guide postsynaptic differentiation. However, less is known about how postsynaptic targets retrogradely regulate presynaptic differentiation or function. We found that muscle-specific conditional knockout of β-catenin (Ctnnb1, also known as β-cat) in mice caused both morphologic and functional defects in motoneuron terminals of neuromuscular junctions (NMJs). In the absence of muscle β-catenin, acetylcholine receptor clusters were increased in size and distributed throughout a wider region. Primary nerve branches were mislocated, whereas secondary or intramuscular nerve branches were elongated and reduced in number. Both spontaneous and evoked neurotransmitter release was reduced at the mutant NMJs. Furthermore, short-term plasticity and calcium sensitivity of neurotransmitter release were compromised in β-catenin-deficient muscle. In contrast, the NMJ was normal in morphology and function in motoneuron-specific β-catenin-deficient mice. Taken together, these observations indicate a role for muscle β-catenin in presynaptic differentiation and function, identifying a previously unknown retrograde signaling in the synapse formation and synaptic plasticity.

Original languageEnglish (US)
Pages (from-to)262-268
Number of pages7
JournalNature Neuroscience
Volume11
Issue number3
DOIs
StatePublished - Mar 1 2008

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Catenins
Motor Neurons
Neuromuscular Junction
Muscles
Synapses
Neurotransmitter Agents
Neuronal Plasticity
Cholinergic Receptors
Cats
Calcium
Neurons

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Li, X. M., Dong, X. P., Luo, S. W., Zhang, B., Lee, D. H., Ting, A. K. L., ... Mei, L. (2008). Retrograde regulation of motoneuron differentiation by muscle β-catenin. Nature Neuroscience, 11(3), 262-268. https://doi.org/10.1038/nn2053

Retrograde regulation of motoneuron differentiation by muscle β-catenin. / Li, Xiao Ming; Dong, Xian Ping; Luo, Shi Wen; Zhang, Bin; Lee, Dae Hoon; Ting, Annie K.L.; Neiswender, Hannah; Kim, Chang Hoon; Carpenter-Hyland, Ezekiel; Gao, Tian Ming; Xiong, Wencheng; Mei, Lin.

In: Nature Neuroscience, Vol. 11, No. 3, 01.03.2008, p. 262-268.

Research output: Contribution to journalArticle

Li, XM, Dong, XP, Luo, SW, Zhang, B, Lee, DH, Ting, AKL, Neiswender, H, Kim, CH, Carpenter-Hyland, E, Gao, TM, Xiong, W & Mei, L 2008, 'Retrograde regulation of motoneuron differentiation by muscle β-catenin', Nature Neuroscience, vol. 11, no. 3, pp. 262-268. https://doi.org/10.1038/nn2053
Li XM, Dong XP, Luo SW, Zhang B, Lee DH, Ting AKL et al. Retrograde regulation of motoneuron differentiation by muscle β-catenin. Nature Neuroscience. 2008 Mar 1;11(3):262-268. https://doi.org/10.1038/nn2053
Li, Xiao Ming ; Dong, Xian Ping ; Luo, Shi Wen ; Zhang, Bin ; Lee, Dae Hoon ; Ting, Annie K.L. ; Neiswender, Hannah ; Kim, Chang Hoon ; Carpenter-Hyland, Ezekiel ; Gao, Tian Ming ; Xiong, Wencheng ; Mei, Lin. / Retrograde regulation of motoneuron differentiation by muscle β-catenin. In: Nature Neuroscience. 2008 ; Vol. 11, No. 3. pp. 262-268.
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