Robo2 determines subtype-specific axonal projections of trigeminal sensory neurons

Y. Albert Pan, Margaret Choy, David A. Prober, Alexander F. Schier

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

How neurons connect to form functional circuits is central to the understanding of the development and function of the nervous system. In the somatosensory system, perception of sensory stimuli to the head requires specific connections between trigeminal sensory neurons and their many target areas in the central nervous system. Different trigeminal subtypes have specialized functions and downstream circuits, but it has remained unclear how subtype-specific axonal projection patterns are formed. Using zebrafish as a model system, we followed the development of two trigeminal sensory neuron subtypes: one that expresses trpa1b, a nociceptive channel important for sensing environmental chemicals; and a distinct subtype labeled by an islet1 reporter (Isl1SS). We found that Trpa1b and Isl1SS neurons have overall similar axon trajectories but different branching morphologies and distributions of presynaptic sites. Compared with Trpa1b neurons, Isl1SS neurons display reduced branch growth and synaptogenesis at the hindbrain-spinal cord junction. The subtype-specific morphogenesis of Isl1SS neurons depends on the guidance receptor Robo2. robo2 is preferentially expressed in the Isl1SS subset and inhibits branch growth and synaptogenesis. In the absence of Robo2, Isl1SS afferents acquire many of the characteristics of Trpa1b afferents. These results reveal that subtypespecific activity of Robo2 regulates subcircuit morphogenesis in the trigeminal sensory system.

Original languageEnglish (US)
Pages (from-to)591-600
Number of pages10
JournalDevelopment
Volume139
Issue number3
DOIs
StatePublished - Feb 1 2012

Fingerprint

Sensory Receptor Cells
Neurons
Morphogenesis
Rhombencephalon
Zebrafish
Growth
Nervous System
Axons
Spinal Cord
Central Nervous System
Head

Keywords

  • Axon guidance
  • Nociception
  • Somatosensory
  • Synaptogenesis
  • TRPA1
  • Targeting
  • Zebrafish

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

Cite this

Robo2 determines subtype-specific axonal projections of trigeminal sensory neurons. / Pan, Y. Albert; Choy, Margaret; Prober, David A.; Schier, Alexander F.

In: Development, Vol. 139, No. 3, 01.02.2012, p. 591-600.

Research output: Contribution to journalArticle

Pan, Y. Albert ; Choy, Margaret ; Prober, David A. ; Schier, Alexander F. / Robo2 determines subtype-specific axonal projections of trigeminal sensory neurons. In: Development. 2012 ; Vol. 139, No. 3. pp. 591-600.
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