Role of dentin MMPs in caries progression and bond stability

A. Mazzoni, L. Tjäderhane, V. Checchi, R. Di Lenarda, T. Salo, Franklin Chi Meng Tay, David Henry Pashley, L. Breschi

Research output: Contribution to journalReview article

83 Citations (Scopus)

Abstract

Dentin can be described as a biological composite with collagen matrix embedded with nanosized hydroxyapatite mineral crystallites. Matrix metalloproteinases (MMPs) and cysteine cathepsins are families of endopeptidases. Enzymes of both families are present in dentin and collectively capable of degrading virtually all extracellular matrix components. This review describes these enzymes and their presence in dentin, mainly focusing on their role in dentin caries pathogenesis and loss of collagen in the adhesive hybrid layer under composite restorations. MMPs and cysteine cathepsins present in saliva, mineralized dentin, and/or dentinal fluid may affect the dentin caries process at the early phases of demineralization. Changes in collagen and noncollagenous protein structure may participate in observed decreases in mechanical properties of caries-affected dentin and reduce the ability of caries-affected dentin to remineralize. These endogenous enzymes also remain entrapped within the hybrid layer during the resin infiltration process, and the acidic bonding agents themselves (irrespective of whether they are etch-and-rinse or self-etch) can activate these endogenous protease proforms. Since resin impregnation is frequently incomplete, denuded collagen matrices associated with free water (which serves as a collagen cleavage reagent for these endogenous hydrolase enzymes) can be enzymatically disrupted, finally contributing to the degradation of the hybrid layer. There are multiple in vitro and in vivo reports showing that the longevity of the adhesive interface is increased when nonspecific enzyme-inhibiting strategies are used. Different chemicals (i.e., chlorhexidine, galardin, and benzalkonium chloride) or collagen cross-linker agents have been successfully employed as therapeutic primers in the bonding procedure. In addition, the incorporation of enzyme inhibitors (i.e., quaternary ammonium methacrylates) into the resin blends has been recently promoted. This review will describe MMP functions in caries and hybrid layer degradation and explore the potential therapeutic role of MMP inhibitors for the development of improved intervention strategies for MMP-related oral diseases.

Original languageEnglish (US)
Pages (from-to)241-251
Number of pages11
JournalJournal of Dental Research
Volume94
Issue number2
DOIs
StatePublished - Jan 1 2015

Fingerprint

Dentin
Matrix Metalloproteinases
Collagen
Enzymes
Cathepsins
Adhesives
Dentinal Fluid
Cysteine
Mouth Diseases
Benzalkonium Compounds
Endopeptidases
Chlorhexidine
Matrix Metalloproteinase Inhibitors
Methacrylates
Enzyme Inhibitors
Hydrolases
Durapatite
Ammonium Compounds
Saliva
Extracellular Matrix

Keywords

  • cathepsins
  • collagen
  • degradation
  • dentin bonding agents
  • enzymes
  • tooth

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Mazzoni, A., Tjäderhane, L., Checchi, V., Di Lenarda, R., Salo, T., Tay, F. C. M., ... Breschi, L. (2015). Role of dentin MMPs in caries progression and bond stability. Journal of Dental Research, 94(2), 241-251. https://doi.org/10.1177/0022034514562833

Role of dentin MMPs in caries progression and bond stability. / Mazzoni, A.; Tjäderhane, L.; Checchi, V.; Di Lenarda, R.; Salo, T.; Tay, Franklin Chi Meng; Pashley, David Henry; Breschi, L.

In: Journal of Dental Research, Vol. 94, No. 2, 01.01.2015, p. 241-251.

Research output: Contribution to journalReview article

Mazzoni, A, Tjäderhane, L, Checchi, V, Di Lenarda, R, Salo, T, Tay, FCM, Pashley, DH & Breschi, L 2015, 'Role of dentin MMPs in caries progression and bond stability', Journal of Dental Research, vol. 94, no. 2, pp. 241-251. https://doi.org/10.1177/0022034514562833
Mazzoni A, Tjäderhane L, Checchi V, Di Lenarda R, Salo T, Tay FCM et al. Role of dentin MMPs in caries progression and bond stability. Journal of Dental Research. 2015 Jan 1;94(2):241-251. https://doi.org/10.1177/0022034514562833
Mazzoni, A. ; Tjäderhane, L. ; Checchi, V. ; Di Lenarda, R. ; Salo, T. ; Tay, Franklin Chi Meng ; Pashley, David Henry ; Breschi, L. / Role of dentin MMPs in caries progression and bond stability. In: Journal of Dental Research. 2015 ; Vol. 94, No. 2. pp. 241-251.
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