Role of gp91phox (Nox2)-containing NAD(P)H oxidase in angiogenesis in response to hindlimb ischemia

Taiki Tojo, Masuko Ushio-Fukai, Minako Yamaoka-Tojo, Satoshi Ikeda, Nikolay Patrushev, R. Wayne Alexander

Research output: Contribution to journalArticle

211 Citations (Scopus)

Abstract

Background - Neovascularization is potentially important for the treatment of ischemic heart and limb disease. We reported that reactive oxygen species (ROS) derived from gp91phox (Nox2)-containing NAD(P)H oxidase are involved in angiogenesis in mouse sponge models as well as in vascular endothelial growth factor (VEGF) signaling in cultured endothelial cells. The role of gp91phox-derived ROS in neovascularization in response to tissue ischemia is unknown, however. Methods and Results - Here, we show that neovascularization in the ischemic hindlimb is significantly impaired in gp91phox-/- mice as compared with wild-type (WT) mice as evaluated by laser Doppler flow, capillary density, and microsphere measurements. In WT mice, inflammatory cell infiltration in the ischemic hindlimb was maximal at 3 days, whereas capillary formation was prominent at 7 days when inflammatory cells were no longer detectable. Increased O2.- production and gp91phox expression were present at both time points. The dihydroethidium staining of ischemic tissues indicates that O2 .- is mainly produced from inflammatory cells at 3 days and from neovasculature at 7 days after operation. Relative to WT mice, ischemia-induced ROS production in gp91phox-/- mice at both 3 and 7 days was diminished, whereas VEGF expression was enhanced and the inflammatory response was unchanged. Infusion of the antioxidant ebselen into WT mice also significantly blocked the increase in blood flow recovery and capillary density after ischemia. Conclusions - gp91phox-derived ROS play an important role in mediating neovascularization in response to tissue ischemia. NAD(P)H oxidases and their products are potential therapeutic targets for regulating angiogenesis in vivo.

Original languageEnglish (US)
Pages (from-to)2347-2355
Number of pages9
JournalCirculation
Volume111
Issue number18
DOIs
StatePublished - May 10 2005

Fingerprint

NADPH Oxidase
Hindlimb
Ischemia
Reactive Oxygen Species
Vascular Endothelial Growth Factor A
Porifera
Microspheres
Myocardial Ischemia
Cultured Cells
Lasers
Extremities
Endothelial Cells
Antioxidants
Staining and Labeling

Keywords

  • Angiogenesis
  • Ischemia
  • NAD(P)H oxidase
  • Reactive oxygen species
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Role of gp91phox (Nox2)-containing NAD(P)H oxidase in angiogenesis in response to hindlimb ischemia. / Tojo, Taiki; Ushio-Fukai, Masuko; Yamaoka-Tojo, Minako; Ikeda, Satoshi; Patrushev, Nikolay; Alexander, R. Wayne.

In: Circulation, Vol. 111, No. 18, 10.05.2005, p. 2347-2355.

Research output: Contribution to journalArticle

Tojo, Taiki ; Ushio-Fukai, Masuko ; Yamaoka-Tojo, Minako ; Ikeda, Satoshi ; Patrushev, Nikolay ; Alexander, R. Wayne. / Role of gp91phox (Nox2)-containing NAD(P)H oxidase in angiogenesis in response to hindlimb ischemia. In: Circulation. 2005 ; Vol. 111, No. 18. pp. 2347-2355.
@article{6a880c454f114e58814247d4dc75c499,
title = "Role of gp91phox (Nox2)-containing NAD(P)H oxidase in angiogenesis in response to hindlimb ischemia",
abstract = "Background - Neovascularization is potentially important for the treatment of ischemic heart and limb disease. We reported that reactive oxygen species (ROS) derived from gp91phox (Nox2)-containing NAD(P)H oxidase are involved in angiogenesis in mouse sponge models as well as in vascular endothelial growth factor (VEGF) signaling in cultured endothelial cells. The role of gp91phox-derived ROS in neovascularization in response to tissue ischemia is unknown, however. Methods and Results - Here, we show that neovascularization in the ischemic hindlimb is significantly impaired in gp91phox-/- mice as compared with wild-type (WT) mice as evaluated by laser Doppler flow, capillary density, and microsphere measurements. In WT mice, inflammatory cell infiltration in the ischemic hindlimb was maximal at 3 days, whereas capillary formation was prominent at 7 days when inflammatory cells were no longer detectable. Increased O2.- production and gp91phox expression were present at both time points. The dihydroethidium staining of ischemic tissues indicates that O2 .- is mainly produced from inflammatory cells at 3 days and from neovasculature at 7 days after operation. Relative to WT mice, ischemia-induced ROS production in gp91phox-/- mice at both 3 and 7 days was diminished, whereas VEGF expression was enhanced and the inflammatory response was unchanged. Infusion of the antioxidant ebselen into WT mice also significantly blocked the increase in blood flow recovery and capillary density after ischemia. Conclusions - gp91phox-derived ROS play an important role in mediating neovascularization in response to tissue ischemia. NAD(P)H oxidases and their products are potential therapeutic targets for regulating angiogenesis in vivo.",
keywords = "Angiogenesis, Ischemia, NAD(P)H oxidase, Reactive oxygen species, Vascular endothelial growth factor",
author = "Taiki Tojo and Masuko Ushio-Fukai and Minako Yamaoka-Tojo and Satoshi Ikeda and Nikolay Patrushev and Alexander, {R. Wayne}",
year = "2005",
month = "5",
day = "10",
doi = "10.1161/01.CIR.0000164261.62586.14",
language = "English (US)",
volume = "111",
pages = "2347--2355",
journal = "Circulation",
issn = "0009-7322",
publisher = "Lippincott Williams and Wilkins",
number = "18",

}

TY - JOUR

T1 - Role of gp91phox (Nox2)-containing NAD(P)H oxidase in angiogenesis in response to hindlimb ischemia

AU - Tojo, Taiki

AU - Ushio-Fukai, Masuko

AU - Yamaoka-Tojo, Minako

AU - Ikeda, Satoshi

AU - Patrushev, Nikolay

AU - Alexander, R. Wayne

PY - 2005/5/10

Y1 - 2005/5/10

N2 - Background - Neovascularization is potentially important for the treatment of ischemic heart and limb disease. We reported that reactive oxygen species (ROS) derived from gp91phox (Nox2)-containing NAD(P)H oxidase are involved in angiogenesis in mouse sponge models as well as in vascular endothelial growth factor (VEGF) signaling in cultured endothelial cells. The role of gp91phox-derived ROS in neovascularization in response to tissue ischemia is unknown, however. Methods and Results - Here, we show that neovascularization in the ischemic hindlimb is significantly impaired in gp91phox-/- mice as compared with wild-type (WT) mice as evaluated by laser Doppler flow, capillary density, and microsphere measurements. In WT mice, inflammatory cell infiltration in the ischemic hindlimb was maximal at 3 days, whereas capillary formation was prominent at 7 days when inflammatory cells were no longer detectable. Increased O2.- production and gp91phox expression were present at both time points. The dihydroethidium staining of ischemic tissues indicates that O2 .- is mainly produced from inflammatory cells at 3 days and from neovasculature at 7 days after operation. Relative to WT mice, ischemia-induced ROS production in gp91phox-/- mice at both 3 and 7 days was diminished, whereas VEGF expression was enhanced and the inflammatory response was unchanged. Infusion of the antioxidant ebselen into WT mice also significantly blocked the increase in blood flow recovery and capillary density after ischemia. Conclusions - gp91phox-derived ROS play an important role in mediating neovascularization in response to tissue ischemia. NAD(P)H oxidases and their products are potential therapeutic targets for regulating angiogenesis in vivo.

AB - Background - Neovascularization is potentially important for the treatment of ischemic heart and limb disease. We reported that reactive oxygen species (ROS) derived from gp91phox (Nox2)-containing NAD(P)H oxidase are involved in angiogenesis in mouse sponge models as well as in vascular endothelial growth factor (VEGF) signaling in cultured endothelial cells. The role of gp91phox-derived ROS in neovascularization in response to tissue ischemia is unknown, however. Methods and Results - Here, we show that neovascularization in the ischemic hindlimb is significantly impaired in gp91phox-/- mice as compared with wild-type (WT) mice as evaluated by laser Doppler flow, capillary density, and microsphere measurements. In WT mice, inflammatory cell infiltration in the ischemic hindlimb was maximal at 3 days, whereas capillary formation was prominent at 7 days when inflammatory cells were no longer detectable. Increased O2.- production and gp91phox expression were present at both time points. The dihydroethidium staining of ischemic tissues indicates that O2 .- is mainly produced from inflammatory cells at 3 days and from neovasculature at 7 days after operation. Relative to WT mice, ischemia-induced ROS production in gp91phox-/- mice at both 3 and 7 days was diminished, whereas VEGF expression was enhanced and the inflammatory response was unchanged. Infusion of the antioxidant ebselen into WT mice also significantly blocked the increase in blood flow recovery and capillary density after ischemia. Conclusions - gp91phox-derived ROS play an important role in mediating neovascularization in response to tissue ischemia. NAD(P)H oxidases and their products are potential therapeutic targets for regulating angiogenesis in vivo.

KW - Angiogenesis

KW - Ischemia

KW - NAD(P)H oxidase

KW - Reactive oxygen species

KW - Vascular endothelial growth factor

UR - http://www.scopus.com/inward/record.url?scp=18444401175&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=18444401175&partnerID=8YFLogxK

U2 - 10.1161/01.CIR.0000164261.62586.14

DO - 10.1161/01.CIR.0000164261.62586.14

M3 - Article

C2 - 15867174

AN - SCOPUS:18444401175

VL - 111

SP - 2347

EP - 2355

JO - Circulation

JF - Circulation

SN - 0009-7322

IS - 18

ER -