Role of modification route for zinc oxide nanoparticles on protein structure and their effects on glioblastoma cells

Mine Altunbek, Seda Keleştemur, Gülin Baran, Mustafa Çulha

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Zinc oxide nanoparticles (ZnO) are presented as potential cancer therapeutic agent based on their surface properties. In this study, the most abundant blood proteins, albumin, fibrinogen and apo-transferrin, were covalently bound (c-ZnO NPs) and nonspecifically adsorbed (n-ZnO NPs) onto ZnO NPs to evaluate the role of modification route on protein structure and their effects on glioblastoma cells. The success of modification and structures of proteins on ZnO NPs were characterized with FT-IR. It was found that non-covalent interaction significantly damaged the secondary structure of proteins compared to those covalently attached to the ZnO nanoparticle. The effects of modified ZnO NPs were investigated by evaluating viability, cycle, and death mechanisms of glioblastoma (U373) cells. n-ZnO NPs were found more toxic compared to the pristine and c-ZnO NPs. However, c-ZnO NPs with albumin and apo-transferrin both perturbed the cell cycle function, and decreased the necrotic cell death rate of U373 cells below toxic concentration, suggesting their potential curative effect on glioblastoma cells.

Original languageEnglish (US)
Pages (from-to)271-278
Number of pages8
JournalInternational Journal of Biological Macromolecules
Volume118
DOIs
StatePublished - Oct 15 2018
Externally publishedYes

Keywords

  • Cell cycle
  • Cell death
  • Glioblastoma
  • Surface chemistry
  • Surface modification
  • Zinc oxide nanoparticle

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Economics and Econometrics
  • Energy(all)

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