Rolling and adhesion of monocytes to early atherosclerotic lesions of apolipoprotein E-/-(apoE-/-) mice requires P-selectin, PSGL-1, α4 integrin and VCAM-1

Yuqing Huo, Carroll L. Ramos, Klaus Ley

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

To investigate the molecular basis of mononuclear cell rolling and adhesions to vascular endothelium prone to develop atherosclerotic lesion, isolated carotid arteries from ApoE-/- and other relevant mice were perfused under physiological shear stress conditions. Carotid arteries from 10-12 weeks old ApoE-/- and C57BL/6 wide-type mice fed a Western diet for 4-5 weeks, representing early atherosclerotic lesions according to vessel histology, supported mononuclear cell (U937) attachment, rolling and adhesion, while carotid arteries from an atherosclerosis-resistant strain (BALB/C) showed no adhesion. Antibody blocking assays showed that mononuclear rolling and adhesion were significantly inhibited by treating the vessel with P-selectin antibody or treating U937 with anti-PSGL-1. Treating the vessel with VCAM-1 antibody or treating U937 with α4β1 integrin antibody caused rolling velocities to increase (from 86±4 to 167±7 um/s). This suggests that mononuclear cell can attach, roll and adhere on early atherosclerotic endothelium via the P-selectin-PSGL-1 and VCAM-1-α4β1 pathways.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
PublisherIEEE
Number of pages1
Volume1
ISBN (Print)0780356756
StatePublished - Dec 1 1999
Externally publishedYes
EventProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS) - Atlanta, GA, USA
Duration: Oct 13 1999Oct 16 1999

Other

OtherProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
CityAtlanta, GA, USA
Period10/13/9910/16/99

Fingerprint

P-Selectin
Vascular Cell Adhesion Molecule-1
Apolipoproteins E
Antibodies
Integrins
Adhesion
Histology
Blocking Antibodies
Nutrition
Shear stress
Assays
Apolipoproteins
Selectins

ASJC Scopus subject areas

  • Bioengineering

Cite this

Huo, Y., Ramos, C. L., & Ley, K. (1999). Rolling and adhesion of monocytes to early atherosclerotic lesions of apolipoprotein E-/-(apoE-/-) mice requires P-selectin, PSGL-1, α4 integrin and VCAM-1. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Vol. 1). IEEE.

Rolling and adhesion of monocytes to early atherosclerotic lesions of apolipoprotein E-/-(apoE-/-) mice requires P-selectin, PSGL-1, α4 integrin and VCAM-1. / Huo, Yuqing; Ramos, Carroll L.; Ley, Klaus.

Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 1 IEEE, 1999.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Huo, Y, Ramos, CL & Ley, K 1999, Rolling and adhesion of monocytes to early atherosclerotic lesions of apolipoprotein E-/-(apoE-/-) mice requires P-selectin, PSGL-1, α4 integrin and VCAM-1. in Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. vol. 1, IEEE, Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS), Atlanta, GA, USA, 10/13/99.
Huo Y, Ramos CL, Ley K. Rolling and adhesion of monocytes to early atherosclerotic lesions of apolipoprotein E-/-(apoE-/-) mice requires P-selectin, PSGL-1, α4 integrin and VCAM-1. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 1. IEEE. 1999
Huo, Yuqing ; Ramos, Carroll L. ; Ley, Klaus. / Rolling and adhesion of monocytes to early atherosclerotic lesions of apolipoprotein E-/-(apoE-/-) mice requires P-selectin, PSGL-1, α4 integrin and VCAM-1. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 1 IEEE, 1999.
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abstract = "To investigate the molecular basis of mononuclear cell rolling and adhesions to vascular endothelium prone to develop atherosclerotic lesion, isolated carotid arteries from ApoE-/- and other relevant mice were perfused under physiological shear stress conditions. Carotid arteries from 10-12 weeks old ApoE-/- and C57BL/6 wide-type mice fed a Western diet for 4-5 weeks, representing early atherosclerotic lesions according to vessel histology, supported mononuclear cell (U937) attachment, rolling and adhesion, while carotid arteries from an atherosclerosis-resistant strain (BALB/C) showed no adhesion. Antibody blocking assays showed that mononuclear rolling and adhesion were significantly inhibited by treating the vessel with P-selectin antibody or treating U937 with anti-PSGL-1. Treating the vessel with VCAM-1 antibody or treating U937 with α4β1 integrin antibody caused rolling velocities to increase (from 86±4 to 167±7 um/s). This suggests that mononuclear cell can attach, roll and adhere on early atherosclerotic endothelium via the P-selectin-PSGL-1 and VCAM-1-α4β1 pathways.",
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