TY - JOUR
T1 - Screening of edible Japanese plants for suppressive effects on phorbol ester-induced superoxide generation in differentiated HL-60 cells and AS52 cells
AU - Kim, Ha Won
AU - Murakami, Akira
AU - Nakamura, Yoshimasa
AU - Ohigashi, Hajime
N1 - Funding Information:
This study was performed with Special Coordination Funds from the Ministry of Education, Culture, Sports, Science and Technology, the Japanese Government, and also through subsidies from Takeda Food Co., Ltd. We thank Dr M.V. Williams of Ohio State University for the generous gift of AS52 cells and Dr R. Sasaki of Kyoto University for his kind donation of HL-60 cells.
PY - 2002/2/8
Y1 - 2002/2/8
N2 - Epithelial xanthine oxidase (XOD) is one of the major enzymes responsible for superoxide (O2-) generation, which is involved in oxidative stress. However, there are few known reports of a convenient bioassay to detect cellular XOD activity. We tested several cell lines, and found that AS52, from Chinese hamster ovary cells, produced a significant level of O2- in response to 12-O-tetradecanoylphorbol 13-acetate (TPA), and this activity was markedly inhibited by allopurinol, an XOD inhibitor. Using AS52 cells and differentiated HL-60 cells, we conducted screening tests of edible Japanese plant extracts for their inhibitory activities toward TPA-induced O2- generation from both reduced nicotinamide adenine dinucleotide oxidase (HL-60) and XOD (AS52). Notably, the extracts from mioga ginger, rape, avocado, carrot, turnip, taro, and shimeji showed potent inhibition of O2- generation in both cell lines. These results suggest that several edible Japanese plants carry a significant antioxidative and cancer preventive potential.
AB - Epithelial xanthine oxidase (XOD) is one of the major enzymes responsible for superoxide (O2-) generation, which is involved in oxidative stress. However, there are few known reports of a convenient bioassay to detect cellular XOD activity. We tested several cell lines, and found that AS52, from Chinese hamster ovary cells, produced a significant level of O2- in response to 12-O-tetradecanoylphorbol 13-acetate (TPA), and this activity was markedly inhibited by allopurinol, an XOD inhibitor. Using AS52 cells and differentiated HL-60 cells, we conducted screening tests of edible Japanese plant extracts for their inhibitory activities toward TPA-induced O2- generation from both reduced nicotinamide adenine dinucleotide oxidase (HL-60) and XOD (AS52). Notably, the extracts from mioga ginger, rape, avocado, carrot, turnip, taro, and shimeji showed potent inhibition of O2- generation in both cell lines. These results suggest that several edible Japanese plants carry a significant antioxidative and cancer preventive potential.
KW - AS52 cells
KW - Cancer chemoprevention
KW - HL-60 cells
KW - Reduced nicotinamide adenine dinucleotide phosphate oxidase
KW - Superoxide
KW - Xanthine oxidase
UR - http://www.scopus.com/inward/record.url?scp=0037039708&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037039708&partnerID=8YFLogxK
U2 - 10.1016/S0304-3835(01)00735-2
DO - 10.1016/S0304-3835(01)00735-2
M3 - Article
C2 - 11790448
AN - SCOPUS:0037039708
VL - 176
SP - 7
EP - 16
JO - Cancer Letters
JF - Cancer Letters
SN - 0304-3835
IS - 1
ER -