Abstract
Epithelial xanthine oxidase (XOD) is one of the major enzymes responsible for superoxide (O2-) generation, which is involved in oxidative stress. However, there are few known reports of a convenient bioassay to detect cellular XOD activity. We tested several cell lines, and found that AS52, from Chinese hamster ovary cells, produced a significant level of O2- in response to 12-O-tetradecanoylphorbol 13-acetate (TPA), and this activity was markedly inhibited by allopurinol, an XOD inhibitor. Using AS52 cells and differentiated HL-60 cells, we conducted screening tests of edible Japanese plant extracts for their inhibitory activities toward TPA-induced O2- generation from both reduced nicotinamide adenine dinucleotide oxidase (HL-60) and XOD (AS52). Notably, the extracts from mioga ginger, rape, avocado, carrot, turnip, taro, and shimeji showed potent inhibition of O2- generation in both cell lines. These results suggest that several edible Japanese plants carry a significant antioxidative and cancer preventive potential.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 7-16 |
| Number of pages | 10 |
| Journal | Cancer Letters |
| Volume | 176 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 8 2002 |
| Externally published | Yes |
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Keywords
- AS52 cells
- Cancer chemoprevention
- HL-60 cells
- Reduced nicotinamide adenine dinucleotide phosphate oxidase
- Superoxide
- Xanthine oxidase
ASJC Scopus subject areas
- Oncology
- Cancer Research
Cite this
Screening of edible Japanese plants for suppressive effects on phorbol ester-induced superoxide generation in differentiated HL-60 cells and AS52 cells. / Kim, Ha Won; Murakami, Akira; Nakamura, Yoshimasa; Ohigashi, Hajime.
In: Cancer Letters, Vol. 176, No. 1, 08.02.2002, p. 7-16.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Screening of edible Japanese plants for suppressive effects on phorbol ester-induced superoxide generation in differentiated HL-60 cells and AS52 cells
AU - Kim, Ha Won
AU - Murakami, Akira
AU - Nakamura, Yoshimasa
AU - Ohigashi, Hajime
PY - 2002/2/8
Y1 - 2002/2/8
N2 - Epithelial xanthine oxidase (XOD) is one of the major enzymes responsible for superoxide (O2-) generation, which is involved in oxidative stress. However, there are few known reports of a convenient bioassay to detect cellular XOD activity. We tested several cell lines, and found that AS52, from Chinese hamster ovary cells, produced a significant level of O2- in response to 12-O-tetradecanoylphorbol 13-acetate (TPA), and this activity was markedly inhibited by allopurinol, an XOD inhibitor. Using AS52 cells and differentiated HL-60 cells, we conducted screening tests of edible Japanese plant extracts for their inhibitory activities toward TPA-induced O2- generation from both reduced nicotinamide adenine dinucleotide oxidase (HL-60) and XOD (AS52). Notably, the extracts from mioga ginger, rape, avocado, carrot, turnip, taro, and shimeji showed potent inhibition of O2- generation in both cell lines. These results suggest that several edible Japanese plants carry a significant antioxidative and cancer preventive potential.
AB - Epithelial xanthine oxidase (XOD) is one of the major enzymes responsible for superoxide (O2-) generation, which is involved in oxidative stress. However, there are few known reports of a convenient bioassay to detect cellular XOD activity. We tested several cell lines, and found that AS52, from Chinese hamster ovary cells, produced a significant level of O2- in response to 12-O-tetradecanoylphorbol 13-acetate (TPA), and this activity was markedly inhibited by allopurinol, an XOD inhibitor. Using AS52 cells and differentiated HL-60 cells, we conducted screening tests of edible Japanese plant extracts for their inhibitory activities toward TPA-induced O2- generation from both reduced nicotinamide adenine dinucleotide oxidase (HL-60) and XOD (AS52). Notably, the extracts from mioga ginger, rape, avocado, carrot, turnip, taro, and shimeji showed potent inhibition of O2- generation in both cell lines. These results suggest that several edible Japanese plants carry a significant antioxidative and cancer preventive potential.
KW - AS52 cells
KW - Cancer chemoprevention
KW - HL-60 cells
KW - Reduced nicotinamide adenine dinucleotide phosphate oxidase
KW - Superoxide
KW - Xanthine oxidase
UR - http://www.scopus.com/inward/record.url?scp=0037039708&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037039708&partnerID=8YFLogxK
U2 - 10.1016/S0304-3835(01)00735-2
DO - 10.1016/S0304-3835(01)00735-2
M3 - Article
C2 - 11790448
AN - SCOPUS:0037039708
VL - 176
SP - 7
EP - 16
JO - Cancer Letters
JF - Cancer Letters
SN - 0304-3835
IS - 1
ER -