TY - JOUR
T1 - Screening of edible Japanese plants for suppressive effects on phorbol ester-induced superoxide generation in differentiated HL-60 cells and AS52 cells
AU - Kim, Ha Won
AU - Murakami, Akira
AU - Nakamura, Yoshimasa
AU - Ohigashi, Hajime
PY - 2002/2/8
Y1 - 2002/2/8
N2 - Epithelial xanthine oxidase (XOD) is one of the major enzymes responsible for superoxide (O2-) generation, which is involved in oxidative stress. However, there are few known reports of a convenient bioassay to detect cellular XOD activity. We tested several cell lines, and found that AS52, from Chinese hamster ovary cells, produced a significant level of O2- in response to 12-O-tetradecanoylphorbol 13-acetate (TPA), and this activity was markedly inhibited by allopurinol, an XOD inhibitor. Using AS52 cells and differentiated HL-60 cells, we conducted screening tests of edible Japanese plant extracts for their inhibitory activities toward TPA-induced O2- generation from both reduced nicotinamide adenine dinucleotide oxidase (HL-60) and XOD (AS52). Notably, the extracts from mioga ginger, rape, avocado, carrot, turnip, taro, and shimeji showed potent inhibition of O2- generation in both cell lines. These results suggest that several edible Japanese plants carry a significant antioxidative and cancer preventive potential.
AB - Epithelial xanthine oxidase (XOD) is one of the major enzymes responsible for superoxide (O2-) generation, which is involved in oxidative stress. However, there are few known reports of a convenient bioassay to detect cellular XOD activity. We tested several cell lines, and found that AS52, from Chinese hamster ovary cells, produced a significant level of O2- in response to 12-O-tetradecanoylphorbol 13-acetate (TPA), and this activity was markedly inhibited by allopurinol, an XOD inhibitor. Using AS52 cells and differentiated HL-60 cells, we conducted screening tests of edible Japanese plant extracts for their inhibitory activities toward TPA-induced O2- generation from both reduced nicotinamide adenine dinucleotide oxidase (HL-60) and XOD (AS52). Notably, the extracts from mioga ginger, rape, avocado, carrot, turnip, taro, and shimeji showed potent inhibition of O2- generation in both cell lines. These results suggest that several edible Japanese plants carry a significant antioxidative and cancer preventive potential.
KW - AS52 cells
KW - Cancer chemoprevention
KW - HL-60 cells
KW - Reduced nicotinamide adenine dinucleotide phosphate oxidase
KW - Superoxide
KW - Xanthine oxidase
UR - http://www.scopus.com/inward/record.url?scp=0037039708&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037039708&partnerID=8YFLogxK
U2 - 10.1016/S0304-3835(01)00735-2
DO - 10.1016/S0304-3835(01)00735-2
M3 - Article
C2 - 11790448
AN - SCOPUS:0037039708
VL - 176
SP - 7
EP - 16
JO - Cancer Letters
JF - Cancer Letters
SN - 0304-3835
IS - 1
ER -