Seasonal change in the number of Cryptosporidium parvum oocysts in water samples from the rivers in Hokkaido, Japan, detected by the ferric sulfate flocculation method

Yoshinori Tsushima, Panagiotis Karanis, Takenori Kamada, Levi H Makala, Xuenan Xuan, Yukinobu Tohya, Hiroomi Akashi, Hideyuki Nagasawa

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

An epidemiological study was carried out in natural water supplies of Hokkaido, one of the largest dairy prefectures in Japan. To investigate the prevalence of Cryptosporidium parvum (C. parvum) oocysts water samples were collected from three rivers in the eastern area of Hokkaido from August 1999 to October 2001, and C. parvum oocysts were collected and purified by the ferric sulfate flocculation method. The oocysts were detected using the immunofluorescent assay test (IFAT) and 4′, 6-diamidino-2-phenylindole (DAPI) staining. The seasonal change in the number of oocysts detected was observed. Oocysts increased in numbers from the late summer to the early autumn (from August to November), thereafter, they exhibited a trend to decrease until December, when no oocysts could be detected. The maximum number of oocysts detected in the three rivers was 3.50, 5.00 and 3.33 oocysts/ l, respectively. The oocyst density in river water changed in relation to the season in 1999, 2000 and 2001. This report first cleared up the seasonal changes in C. parvum oocysts number in river water.

Original languageEnglish (US)
Pages (from-to)121-123
Number of pages3
JournalJournal of Veterinary Medical Science
Volume65
Issue number1
DOIs
StatePublished - Jan 1 2003

Fingerprint

ferric sulfate
Cryptosporidium parvum
Flocculation
Oocysts
flocculation
oocysts
Rivers
Japan
rivers
Water
water
sampling
methodology
river water
Water Supply
epidemiological studies
fluorescent antibody technique
water supply
Epidemiologic Studies
dairies

Keywords

  • Cryptosporidium parvum oocyst
  • River water
  • Seasonal change

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Seasonal change in the number of Cryptosporidium parvum oocysts in water samples from the rivers in Hokkaido, Japan, detected by the ferric sulfate flocculation method. / Tsushima, Yoshinori; Karanis, Panagiotis; Kamada, Takenori; Makala, Levi H; Xuan, Xuenan; Tohya, Yukinobu; Akashi, Hiroomi; Nagasawa, Hideyuki.

In: Journal of Veterinary Medical Science, Vol. 65, No. 1, 01.01.2003, p. 121-123.

Research output: Contribution to journalArticle

Tsushima, Yoshinori ; Karanis, Panagiotis ; Kamada, Takenori ; Makala, Levi H ; Xuan, Xuenan ; Tohya, Yukinobu ; Akashi, Hiroomi ; Nagasawa, Hideyuki. / Seasonal change in the number of Cryptosporidium parvum oocysts in water samples from the rivers in Hokkaido, Japan, detected by the ferric sulfate flocculation method. In: Journal of Veterinary Medical Science. 2003 ; Vol. 65, No. 1. pp. 121-123.
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