Second trimester amniotic fluid is reliably fern positive and α1-microglobulin positive

Dispelling a labor deck myth

Nicholas S. Fogelson, Paul Christopher Browne, Stanette Browne, Anthony R. Gregg

Research output: Contribution to journalArticle

Abstract

Objective Many physicians erroneously believe and have propagated the myth that a second trimester amniotic fluid sample will not fern. We attempted to redemonstrate ferning and to demonstrate the efficacy of an assay for α1-microglobulin in second trimester amniotic fluid samples. Study Design Amniotic fluid samples were collected from women undergoing routine genetic amniocentesis between 15 and 26 weeks. A total of 97 samples of second trimester amniotic fluid were placed on slides and examined for ferning at 5 and 10 minutes. An α1-microglobulin assay was performed on each sample. Data were recorded and reported. Results Approximately 93% of samples were fern positive at 5 minutes and 100% were fern positive at 10 minutes. All samples were α1-microglobulin positive. Conclusion Pure amniotic fluid is reliably fern and α1-microglobulin positive in the second trimester. Republication of data only available in pre-online sources will benefit physicians and patients.

Original languageEnglish (US)
Pages (from-to)389-392
Number of pages4
JournalAmerican Journal of Perinatology
Volume31
Issue number5
DOIs
StatePublished - Jan 1 2014

Fingerprint

Ferns
Second Pregnancy Trimester
Amniotic Fluid
Physicians
Amniocentesis

Keywords

  • amniotic fluid
  • ferning
  • ruptured membranes

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Obstetrics and Gynecology

Cite this

Second trimester amniotic fluid is reliably fern positive and α1-microglobulin positive : Dispelling a labor deck myth. / Fogelson, Nicholas S.; Browne, Paul Christopher; Browne, Stanette; Gregg, Anthony R.

In: American Journal of Perinatology, Vol. 31, No. 5, 01.01.2014, p. 389-392.

Research output: Contribution to journalArticle

Fogelson, Nicholas S. ; Browne, Paul Christopher ; Browne, Stanette ; Gregg, Anthony R. / Second trimester amniotic fluid is reliably fern positive and α1-microglobulin positive : Dispelling a labor deck myth. In: American Journal of Perinatology. 2014 ; Vol. 31, No. 5. pp. 389-392.
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AB - Objective Many physicians erroneously believe and have propagated the myth that a second trimester amniotic fluid sample will not fern. We attempted to redemonstrate ferning and to demonstrate the efficacy of an assay for α1-microglobulin in second trimester amniotic fluid samples. Study Design Amniotic fluid samples were collected from women undergoing routine genetic amniocentesis between 15 and 26 weeks. A total of 97 samples of second trimester amniotic fluid were placed on slides and examined for ferning at 5 and 10 minutes. An α1-microglobulin assay was performed on each sample. Data were recorded and reported. Results Approximately 93% of samples were fern positive at 5 minutes and 100% were fern positive at 10 minutes. All samples were α1-microglobulin positive. Conclusion Pure amniotic fluid is reliably fern and α1-microglobulin positive in the second trimester. Republication of data only available in pre-online sources will benefit physicians and patients.

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