Segregated responses of mammary gland development and vaginal opening to prepubertal genistein exposure in Bscl2-/- female mice with lipodystrophy

Rong Li, Ahmed E. El Zowalaty, Weiqin Chen, Elizabeth A. Dudley, Xiaoqin Ye

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Berardinelli-Seip congenital lipodystrophy 2-deficient (Bscl2-/-) mice recapitulate human BSCL2 disease with lipodystrophy. Bscl2-encoded seipin is detected in adipocytes and epithelium of mammary gland. Postnatal mammary gland growth spurt and vaginal opening signify pubertal onset in female mice. Bscl2-/- females have longer and dilated mammary gland ducts at 5-week old and delayed vaginal opening. Prepubertal exposure to 500ppm genistein diet increases mammary gland area and accelerates vaginal opening in both control and Bscl2-/- females. However, genistein treatment increases ductal length in control but not Bscl2-/- females. Neither prepubertal genistein treatment nor Bscl2-deficiency affects phospho-estrogen receptor α or progesterone receptor expression patterns in 5-week old mammary gland. Interestingly, Bscl2-deficiency specifically reduces estrogen receptor β expression in mammary gland ductal epithelium. In summary, Bscl2-/- females have accelerated postnatal mammary ductal development but delayed vaginal opening; they display segregated responses in mammary gland development and vaginal opening to prepubertal genistein treatment.

Original languageEnglish (US)
Pages (from-to)76-83
Number of pages8
JournalReproductive Toxicology
Volume54
DOIs
StatePublished - Jul 1 2015

Keywords

  • Bscl2/Seipin
  • Genistein
  • Lipodystrophy
  • Mammary gland
  • Puberty
  • Vaginal opening

ASJC Scopus subject areas

  • Toxicology

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