Selection of specific phage from display libraries: Monoclonal antibody against VCS M13 helper phage coat protein III (gIIIp)

Julie Lin, Kumiko Yanase, Abraham Rutgers, Michael P. Madaio, Kevin E.C. Meyers

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Screening of specific phage is often hampered by nonspecific binding either of the VCS M13 helper phage to the solid phase absorbent or to the polyclonal antibodies used for selection. The former is improved by increasing the stringency for selection. However, the available polyclonal anti-VCS M13 antibodies often react with immobilized antigen nonspecifically, making it difficult to distinguish between positive and negative clones. To improve this selection process, a monoclonal antibody (MAb) was produced which recognizes ligand-coat protein three (gIIIp) on the helper phage VCS M13. This MAb is highly sensitive and specific, and it is useful for selecting relevant clones. This reagent should find widespread application in identifying interactive clones from a variety of phage display libraries.

Original languageEnglish (US)
Pages (from-to)257-261
Number of pages5
JournalHybridoma
Volume18
Issue number3
DOIs
StatePublished - Jun 1 1999

Fingerprint

Bacteriophage M13
Capsid Proteins
Bacteriophages
Libraries
Clone Cells
Monoclonal Antibodies
Antibodies
Ligands
Antigens

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Selection of specific phage from display libraries : Monoclonal antibody against VCS M13 helper phage coat protein III (gIIIp). / Lin, Julie; Yanase, Kumiko; Rutgers, Abraham; Madaio, Michael P.; Meyers, Kevin E.C.

In: Hybridoma, Vol. 18, No. 3, 01.06.1999, p. 257-261.

Research output: Contribution to journalArticle

Lin, Julie ; Yanase, Kumiko ; Rutgers, Abraham ; Madaio, Michael P. ; Meyers, Kevin E.C. / Selection of specific phage from display libraries : Monoclonal antibody against VCS M13 helper phage coat protein III (gIIIp). In: Hybridoma. 1999 ; Vol. 18, No. 3. pp. 257-261.
@article{78c59e022c4a42f4a60b321368c790c0,
title = "Selection of specific phage from display libraries: Monoclonal antibody against VCS M13 helper phage coat protein III (gIIIp)",
abstract = "Screening of specific phage is often hampered by nonspecific binding either of the VCS M13 helper phage to the solid phase absorbent or to the polyclonal antibodies used for selection. The former is improved by increasing the stringency for selection. However, the available polyclonal anti-VCS M13 antibodies often react with immobilized antigen nonspecifically, making it difficult to distinguish between positive and negative clones. To improve this selection process, a monoclonal antibody (MAb) was produced which recognizes ligand-coat protein three (gIIIp) on the helper phage VCS M13. This MAb is highly sensitive and specific, and it is useful for selecting relevant clones. This reagent should find widespread application in identifying interactive clones from a variety of phage display libraries.",
author = "Julie Lin and Kumiko Yanase and Abraham Rutgers and Madaio, {Michael P.} and Meyers, {Kevin E.C.}",
year = "1999",
month = "6",
day = "1",
doi = "10.1089/027245799315916",
language = "English (US)",
volume = "18",
pages = "257--261",
journal = "Monoclonal Antibodies in Immunodiagnosis and Immunotherapy",
issn = "2167-9436",
publisher = "Mary Ann Liebert Inc.",
number = "3",

}

TY - JOUR

T1 - Selection of specific phage from display libraries

T2 - Monoclonal antibody against VCS M13 helper phage coat protein III (gIIIp)

AU - Lin, Julie

AU - Yanase, Kumiko

AU - Rutgers, Abraham

AU - Madaio, Michael P.

AU - Meyers, Kevin E.C.

PY - 1999/6/1

Y1 - 1999/6/1

N2 - Screening of specific phage is often hampered by nonspecific binding either of the VCS M13 helper phage to the solid phase absorbent or to the polyclonal antibodies used for selection. The former is improved by increasing the stringency for selection. However, the available polyclonal anti-VCS M13 antibodies often react with immobilized antigen nonspecifically, making it difficult to distinguish between positive and negative clones. To improve this selection process, a monoclonal antibody (MAb) was produced which recognizes ligand-coat protein three (gIIIp) on the helper phage VCS M13. This MAb is highly sensitive and specific, and it is useful for selecting relevant clones. This reagent should find widespread application in identifying interactive clones from a variety of phage display libraries.

AB - Screening of specific phage is often hampered by nonspecific binding either of the VCS M13 helper phage to the solid phase absorbent or to the polyclonal antibodies used for selection. The former is improved by increasing the stringency for selection. However, the available polyclonal anti-VCS M13 antibodies often react with immobilized antigen nonspecifically, making it difficult to distinguish between positive and negative clones. To improve this selection process, a monoclonal antibody (MAb) was produced which recognizes ligand-coat protein three (gIIIp) on the helper phage VCS M13. This MAb is highly sensitive and specific, and it is useful for selecting relevant clones. This reagent should find widespread application in identifying interactive clones from a variety of phage display libraries.

UR - http://www.scopus.com/inward/record.url?scp=0032812195&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032812195&partnerID=8YFLogxK

U2 - 10.1089/027245799315916

DO - 10.1089/027245799315916

M3 - Article

C2 - 10475240

AN - SCOPUS:0032812195

VL - 18

SP - 257

EP - 261

JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

JF - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

SN - 2167-9436

IS - 3

ER -