Selective demineralisation of dentine extrafibrillar minerals—A potential method to eliminate water-wet bonding in the etch-and-rinse technique

Bingqing Li, Xiaoming Zhu, Lin Ma, Fangping Wang, Xiaoqiang Liu, Xu Yang, Jianfeng Zhou, Jianguo Tan, David Henry Pashley, Franklin Chi Meng Tay

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objective The present study tested the central hypothesis that selective demineralisation of dentine extrafibrillar minerals by lowering the phosphoric acid concentration improves the quality of the resin-dentine interface. Methods Dentine surfaces were etched with different concentrations of phosphoric acid (1, 5, 10, 20, 30 or 40 wt%). Scanning electron microscopy was used to observe the micromorphology of the etched dentine surfaces. Energy dispersive X-ray analysis was performed to determine the residual Ca-content of the demineralised dentine matrix. Atomic force microscopy-based nanoindentation was used to analyse the nanomechanical properties of the treated dentine surfaces. The influence of H3PO4 concentration on resin-dentine bond strength was evaluated by microtensile bond strength testing. One-way ANOVA was used to compare the residual Ca-content ratio, reduced elastic modulus (Er) of the treated dentine surfaces and microtensile bond strength among groups. Results Collagen fibrils appeared to be wider in diameter after etching with 5% and 10% H3PO4. The partially-demineralized collagen scaffold retained part of its rigidity to maintain an uncollapsed three-dimensional structure. Etching with 1% H3PO4 resulted in the highest residual Ca-content ratio and Er of demineralised dentine matrix, followed by 5% H3PO4. Those values were all significantly higher than values derived from the other groups. Etching with 30% H3PO4 resulted in the lowest Ca-content ratio and Er. Using 5% H3PO4 as etchant resulted in the highest resin-dentine bond strength. Conclusions Selective demineralisation of the dentine matrix may be achieved by lowering the H3PO4 concentration to 5 wt%, to achieve better bonding performance. Clinical relevance By retaining intrafibrillar minerals, more through air-drying of the partially demineralised collagen matrix may be accomplished without the need to worry about collapsing a mineral-free collagen matrix during air-drying. This may result in the elimination of water-wet bonding during the application of etch-and-rinse adhesives.

Original languageEnglish (US)
Pages (from-to)55-62
Number of pages8
JournalJournal of Dentistry
Volume52
DOIs
StatePublished - Sep 1 2016

Fingerprint

Dentin
Water
Collagen
Minerals
Air
Atomic Force Microscopy
Elastic Modulus
Adhesives
Electron Scanning Microscopy
Analysis of Variance
X-Rays

Keywords

  • Collagen matrix
  • Etch-and-rinse
  • Interfibrillar space
  • Intrafibrillar mineral
  • Selective demineralisation

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Selective demineralisation of dentine extrafibrillar minerals—A potential method to eliminate water-wet bonding in the etch-and-rinse technique. / Li, Bingqing; Zhu, Xiaoming; Ma, Lin; Wang, Fangping; Liu, Xiaoqiang; Yang, Xu; Zhou, Jianfeng; Tan, Jianguo; Pashley, David Henry; Tay, Franklin Chi Meng.

In: Journal of Dentistry, Vol. 52, 01.09.2016, p. 55-62.

Research output: Contribution to journalArticle

Li, Bingqing ; Zhu, Xiaoming ; Ma, Lin ; Wang, Fangping ; Liu, Xiaoqiang ; Yang, Xu ; Zhou, Jianfeng ; Tan, Jianguo ; Pashley, David Henry ; Tay, Franklin Chi Meng. / Selective demineralisation of dentine extrafibrillar minerals—A potential method to eliminate water-wet bonding in the etch-and-rinse technique. In: Journal of Dentistry. 2016 ; Vol. 52. pp. 55-62.
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abstract = "Objective The present study tested the central hypothesis that selective demineralisation of dentine extrafibrillar minerals by lowering the phosphoric acid concentration improves the quality of the resin-dentine interface. Methods Dentine surfaces were etched with different concentrations of phosphoric acid (1, 5, 10, 20, 30 or 40 wt{\%}). Scanning electron microscopy was used to observe the micromorphology of the etched dentine surfaces. Energy dispersive X-ray analysis was performed to determine the residual Ca-content of the demineralised dentine matrix. Atomic force microscopy-based nanoindentation was used to analyse the nanomechanical properties of the treated dentine surfaces. The influence of H3PO4 concentration on resin-dentine bond strength was evaluated by microtensile bond strength testing. One-way ANOVA was used to compare the residual Ca-content ratio, reduced elastic modulus (Er) of the treated dentine surfaces and microtensile bond strength among groups. Results Collagen fibrils appeared to be wider in diameter after etching with 5{\%} and 10{\%} H3PO4. The partially-demineralized collagen scaffold retained part of its rigidity to maintain an uncollapsed three-dimensional structure. Etching with 1{\%} H3PO4 resulted in the highest residual Ca-content ratio and Er of demineralised dentine matrix, followed by 5{\%} H3PO4. Those values were all significantly higher than values derived from the other groups. Etching with 30{\%} H3PO4 resulted in the lowest Ca-content ratio and Er. Using 5{\%} H3PO4 as etchant resulted in the highest resin-dentine bond strength. Conclusions Selective demineralisation of the dentine matrix may be achieved by lowering the H3PO4 concentration to 5 wt{\%}, to achieve better bonding performance. Clinical relevance By retaining intrafibrillar minerals, more through air-drying of the partially demineralised collagen matrix may be accomplished without the need to worry about collapsing a mineral-free collagen matrix during air-drying. This may result in the elimination of water-wet bonding during the application of etch-and-rinse adhesives.",
keywords = "Collagen matrix, Etch-and-rinse, Interfibrillar space, Intrafibrillar mineral, Selective demineralisation",
author = "Bingqing Li and Xiaoming Zhu and Lin Ma and Fangping Wang and Xiaoqiang Liu and Xu Yang and Jianfeng Zhou and Jianguo Tan and Pashley, {David Henry} and Tay, {Franklin Chi Meng}",
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AU - Li, Bingqing

AU - Zhu, Xiaoming

AU - Ma, Lin

AU - Wang, Fangping

AU - Liu, Xiaoqiang

AU - Yang, Xu

AU - Zhou, Jianfeng

AU - Tan, Jianguo

AU - Pashley, David Henry

AU - Tay, Franklin Chi Meng

PY - 2016/9/1

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N2 - Objective The present study tested the central hypothesis that selective demineralisation of dentine extrafibrillar minerals by lowering the phosphoric acid concentration improves the quality of the resin-dentine interface. Methods Dentine surfaces were etched with different concentrations of phosphoric acid (1, 5, 10, 20, 30 or 40 wt%). Scanning electron microscopy was used to observe the micromorphology of the etched dentine surfaces. Energy dispersive X-ray analysis was performed to determine the residual Ca-content of the demineralised dentine matrix. Atomic force microscopy-based nanoindentation was used to analyse the nanomechanical properties of the treated dentine surfaces. The influence of H3PO4 concentration on resin-dentine bond strength was evaluated by microtensile bond strength testing. One-way ANOVA was used to compare the residual Ca-content ratio, reduced elastic modulus (Er) of the treated dentine surfaces and microtensile bond strength among groups. Results Collagen fibrils appeared to be wider in diameter after etching with 5% and 10% H3PO4. The partially-demineralized collagen scaffold retained part of its rigidity to maintain an uncollapsed three-dimensional structure. Etching with 1% H3PO4 resulted in the highest residual Ca-content ratio and Er of demineralised dentine matrix, followed by 5% H3PO4. Those values were all significantly higher than values derived from the other groups. Etching with 30% H3PO4 resulted in the lowest Ca-content ratio and Er. Using 5% H3PO4 as etchant resulted in the highest resin-dentine bond strength. Conclusions Selective demineralisation of the dentine matrix may be achieved by lowering the H3PO4 concentration to 5 wt%, to achieve better bonding performance. Clinical relevance By retaining intrafibrillar minerals, more through air-drying of the partially demineralised collagen matrix may be accomplished without the need to worry about collapsing a mineral-free collagen matrix during air-drying. This may result in the elimination of water-wet bonding during the application of etch-and-rinse adhesives.

AB - Objective The present study tested the central hypothesis that selective demineralisation of dentine extrafibrillar minerals by lowering the phosphoric acid concentration improves the quality of the resin-dentine interface. Methods Dentine surfaces were etched with different concentrations of phosphoric acid (1, 5, 10, 20, 30 or 40 wt%). Scanning electron microscopy was used to observe the micromorphology of the etched dentine surfaces. Energy dispersive X-ray analysis was performed to determine the residual Ca-content of the demineralised dentine matrix. Atomic force microscopy-based nanoindentation was used to analyse the nanomechanical properties of the treated dentine surfaces. The influence of H3PO4 concentration on resin-dentine bond strength was evaluated by microtensile bond strength testing. One-way ANOVA was used to compare the residual Ca-content ratio, reduced elastic modulus (Er) of the treated dentine surfaces and microtensile bond strength among groups. Results Collagen fibrils appeared to be wider in diameter after etching with 5% and 10% H3PO4. The partially-demineralized collagen scaffold retained part of its rigidity to maintain an uncollapsed three-dimensional structure. Etching with 1% H3PO4 resulted in the highest residual Ca-content ratio and Er of demineralised dentine matrix, followed by 5% H3PO4. Those values were all significantly higher than values derived from the other groups. Etching with 30% H3PO4 resulted in the lowest Ca-content ratio and Er. Using 5% H3PO4 as etchant resulted in the highest resin-dentine bond strength. Conclusions Selective demineralisation of the dentine matrix may be achieved by lowering the H3PO4 concentration to 5 wt%, to achieve better bonding performance. Clinical relevance By retaining intrafibrillar minerals, more through air-drying of the partially demineralised collagen matrix may be accomplished without the need to worry about collapsing a mineral-free collagen matrix during air-drying. This may result in the elimination of water-wet bonding during the application of etch-and-rinse adhesives.

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KW - Etch-and-rinse

KW - Interfibrillar space

KW - Intrafibrillar mineral

KW - Selective demineralisation

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