Sequence specific interaction of the chromosomal proteins with DNA

Dorothy Tuan, Christopher Chetsanga, Paul M. Doty

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Calf thymus chromatin was partially deproteinized by 0.6-1 M NaCl extraction. From the shape of the temperature derivative plot of its melting curve, the DNA in each chromatin was resolved into regions of exposed DNA, and DNA still complexed with proteins. The partially deproteinized chromatin was capable of directing in vitroRNA synthesis in an amount proportional to the fraction of exposed DNA. The in vitro RNA transcript was hybridized to denatured calf DNA under hybridization conditions where only RNA's transcribed from repetitive DNA sequences were able to from hybrids. From the results, it is deduced that the in vitroRNA transcripts of 0.6 M NaCl-extracted chromatin after the removal of HI histones plus one-quarter of the non histones may be transcribed from repetitive DNA sequences and those of 1 M NaCl-extracted chromatin contain more transcripts from the unique DNA sequences.

Original languageEnglish (US)
Pages (from-to)3415-3439
Number of pages25
JournalNucleic Acids Research
Volume4
Issue number10
DOIs
StatePublished - Oct 1 1977

ASJC Scopus subject areas

  • Genetics

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