Serum cytokines as biomarkers for nonsurgical prediction of endometriosis

Essam El Din R. Othman, Daniela Hornung, Hosam T. Salem, Essam A. Khalifa, Tarek H. El-Metwally, Ayman Al-Hendy

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

Objective: To test the ability of a group of serum cytokines, either individually or in combination, to serve as biomarkers for the nonsurgical diagnosis of endometriosis. Study design: Subjects were allocated to two groups according to their laparoscopic diagnosis. The first group consisted of patients with endometriosis and the second group was made up of infertile women with no pelvic pathology (controls). Blood samples were collected preoperatively and stored. Cytokines were measured in the serum of all participants using the Bio-Plex Protein Array System. Nonparametric statistics and the Mann-Whitney test were used to compare groups. Subjects were seen at the Gynecologic endoscopy unit. Results: Three cytokines were significantly higher in the serum of subjects with endometriosis than in the control group: interleukin-6 (IL-6) [4.41 pg/ml (range: 1.47-15.01) versus 0.97 pg/ml (range: 0.29-2.98), respectively; p < 0.001], monocyte chemotactic protein-1 (MCP-1) [37.91 pg/ml (range: 24.54-94.74) versus 22.13 pg/ml (range: 13.85-39.45), respectively; p < 0.001], and interferon-gamma (INF-γ) [19.01 pg/ml (range: 1.19-73.52) versus 0.30 pg/ml (range: 0.00-13.05), respectively; p < 0.001]. There was no statistically significant difference between subjects with endometriosis and controls in the serum concentration of vascular endothelial growth factor (VEGF), tumor necrosis factor-alpha (TNF-α), or granulocyte macrophage colony stimulating factor (GM-CSF). Interleukin-2 (IL-2), interleukin-8 (IL-8), and interleukin-15 (IL-15) were undetectable in the serum of both groups. None of the measured cytokines showed significant correlation with the cycle phase or stage of endometriosis. In a multivariate analysis, serum interleukin-6 provided a sensitivity of 71% and a specificity of 66% to discriminate between endometriosis patients and controls at a cutoff point of 1.9 pg/ml. Adding monocyte chemotactic protein-1 and interferon-gamma to interleukin-6 did not increase the discriminative ability over that achieved by measuring serum interleukin-6 alone. Conclusions: Serum of subjects with endometriosis contains significantly higher levels of interleukin-6, monocyte chemotactic protein-1, and interferon-gamma than control women. Serum interleukin-6 measurements discriminate between women with endometriosis and controls. Interleukin-6 provides a promising serum marker for the nonsurgical prediction of endometriosis.

Original languageEnglish (US)
Pages (from-to)240-246
Number of pages7
JournalEuropean Journal of Obstetrics Gynecology and Reproductive Biology
Volume137
Issue number2
DOIs
StatePublished - Apr 1 2008

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Endometriosis
Biomarkers
Cytokines
Interleukin-6
Serum
Chemokine CCL2
Interferon-gamma
Interleukin-15
Protein Array Analysis
Granulocyte-Macrophage Colony-Stimulating Factor
Nonparametric Statistics
Interleukin-8
Vascular Endothelial Growth Factor A
Endoscopy
Interleukin-2
Multivariate Analysis
Tumor Necrosis Factor-alpha
Pathology
Control Groups

Keywords

  • Cytokines
  • Endometriosis
  • IFN-γ
  • IL-6
  • MCP-1
  • Serum

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

Cite this

Othman, E. E. D. R., Hornung, D., Salem, H. T., Khalifa, E. A., El-Metwally, T. H., & Al-Hendy, A. (2008). Serum cytokines as biomarkers for nonsurgical prediction of endometriosis. European Journal of Obstetrics Gynecology and Reproductive Biology, 137(2), 240-246. https://doi.org/10.1016/j.ejogrb.2007.05.001

Serum cytokines as biomarkers for nonsurgical prediction of endometriosis. / Othman, Essam El Din R.; Hornung, Daniela; Salem, Hosam T.; Khalifa, Essam A.; El-Metwally, Tarek H.; Al-Hendy, Ayman.

In: European Journal of Obstetrics Gynecology and Reproductive Biology, Vol. 137, No. 2, 01.04.2008, p. 240-246.

Research output: Contribution to journalArticle

Othman, EEDR, Hornung, D, Salem, HT, Khalifa, EA, El-Metwally, TH & Al-Hendy, A 2008, 'Serum cytokines as biomarkers for nonsurgical prediction of endometriosis', European Journal of Obstetrics Gynecology and Reproductive Biology, vol. 137, no. 2, pp. 240-246. https://doi.org/10.1016/j.ejogrb.2007.05.001
Othman, Essam El Din R. ; Hornung, Daniela ; Salem, Hosam T. ; Khalifa, Essam A. ; El-Metwally, Tarek H. ; Al-Hendy, Ayman. / Serum cytokines as biomarkers for nonsurgical prediction of endometriosis. In: European Journal of Obstetrics Gynecology and Reproductive Biology. 2008 ; Vol. 137, No. 2. pp. 240-246.
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abstract = "Objective: To test the ability of a group of serum cytokines, either individually or in combination, to serve as biomarkers for the nonsurgical diagnosis of endometriosis. Study design: Subjects were allocated to two groups according to their laparoscopic diagnosis. The first group consisted of patients with endometriosis and the second group was made up of infertile women with no pelvic pathology (controls). Blood samples were collected preoperatively and stored. Cytokines were measured in the serum of all participants using the Bio-Plex Protein Array System. Nonparametric statistics and the Mann-Whitney test were used to compare groups. Subjects were seen at the Gynecologic endoscopy unit. Results: Three cytokines were significantly higher in the serum of subjects with endometriosis than in the control group: interleukin-6 (IL-6) [4.41 pg/ml (range: 1.47-15.01) versus 0.97 pg/ml (range: 0.29-2.98), respectively; p < 0.001], monocyte chemotactic protein-1 (MCP-1) [37.91 pg/ml (range: 24.54-94.74) versus 22.13 pg/ml (range: 13.85-39.45), respectively; p < 0.001], and interferon-gamma (INF-γ) [19.01 pg/ml (range: 1.19-73.52) versus 0.30 pg/ml (range: 0.00-13.05), respectively; p < 0.001]. There was no statistically significant difference between subjects with endometriosis and controls in the serum concentration of vascular endothelial growth factor (VEGF), tumor necrosis factor-alpha (TNF-α), or granulocyte macrophage colony stimulating factor (GM-CSF). Interleukin-2 (IL-2), interleukin-8 (IL-8), and interleukin-15 (IL-15) were undetectable in the serum of both groups. None of the measured cytokines showed significant correlation with the cycle phase or stage of endometriosis. In a multivariate analysis, serum interleukin-6 provided a sensitivity of 71{\%} and a specificity of 66{\%} to discriminate between endometriosis patients and controls at a cutoff point of 1.9 pg/ml. Adding monocyte chemotactic protein-1 and interferon-gamma to interleukin-6 did not increase the discriminative ability over that achieved by measuring serum interleukin-6 alone. Conclusions: Serum of subjects with endometriosis contains significantly higher levels of interleukin-6, monocyte chemotactic protein-1, and interferon-gamma than control women. Serum interleukin-6 measurements discriminate between women with endometriosis and controls. Interleukin-6 provides a promising serum marker for the nonsurgical prediction of endometriosis.",
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AU - Othman, Essam El Din R.

AU - Hornung, Daniela

AU - Salem, Hosam T.

AU - Khalifa, Essam A.

AU - El-Metwally, Tarek H.

AU - Al-Hendy, Ayman

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N2 - Objective: To test the ability of a group of serum cytokines, either individually or in combination, to serve as biomarkers for the nonsurgical diagnosis of endometriosis. Study design: Subjects were allocated to two groups according to their laparoscopic diagnosis. The first group consisted of patients with endometriosis and the second group was made up of infertile women with no pelvic pathology (controls). Blood samples were collected preoperatively and stored. Cytokines were measured in the serum of all participants using the Bio-Plex Protein Array System. Nonparametric statistics and the Mann-Whitney test were used to compare groups. Subjects were seen at the Gynecologic endoscopy unit. Results: Three cytokines were significantly higher in the serum of subjects with endometriosis than in the control group: interleukin-6 (IL-6) [4.41 pg/ml (range: 1.47-15.01) versus 0.97 pg/ml (range: 0.29-2.98), respectively; p < 0.001], monocyte chemotactic protein-1 (MCP-1) [37.91 pg/ml (range: 24.54-94.74) versus 22.13 pg/ml (range: 13.85-39.45), respectively; p < 0.001], and interferon-gamma (INF-γ) [19.01 pg/ml (range: 1.19-73.52) versus 0.30 pg/ml (range: 0.00-13.05), respectively; p < 0.001]. There was no statistically significant difference between subjects with endometriosis and controls in the serum concentration of vascular endothelial growth factor (VEGF), tumor necrosis factor-alpha (TNF-α), or granulocyte macrophage colony stimulating factor (GM-CSF). Interleukin-2 (IL-2), interleukin-8 (IL-8), and interleukin-15 (IL-15) were undetectable in the serum of both groups. None of the measured cytokines showed significant correlation with the cycle phase or stage of endometriosis. In a multivariate analysis, serum interleukin-6 provided a sensitivity of 71% and a specificity of 66% to discriminate between endometriosis patients and controls at a cutoff point of 1.9 pg/ml. Adding monocyte chemotactic protein-1 and interferon-gamma to interleukin-6 did not increase the discriminative ability over that achieved by measuring serum interleukin-6 alone. Conclusions: Serum of subjects with endometriosis contains significantly higher levels of interleukin-6, monocyte chemotactic protein-1, and interferon-gamma than control women. Serum interleukin-6 measurements discriminate between women with endometriosis and controls. Interleukin-6 provides a promising serum marker for the nonsurgical prediction of endometriosis.

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