Setting properties and cytotoxicity evaluation of a premixed bioceramic root canal sealer

Bethany A. Loushine, Thomas E. Bryan, Stephen Warwick Looney, Brian M. Gillen, Robert J. Loushine, R. Norman Weller, David Henry Pashley, Franklin Chi Meng Tay

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

Introduction: This study investigated the setting time and micohardness of a premixed calcium phosphate silicate-based sealer (EndoSequence BC Sealer; Brasseler USA, Savannah, GA) in the presence of different moisture contents (0-9 wt%). The moisture content that produced the most optimal setting properties was used to prepare set EndoSequence BC Sealer for cytotoxicity comparison with an epoxy resin-based sealer (AH Plus; Dentsply Caulk, Milford, DE). Methods: Standardized disks were created with BC Sealer, AH Plus, Pulp Canal Sealer EWT (positive control) (SybronEndo, Orange CA), and Teflon (Small Parts Inc., Miami Lakes, FL; negative control). Disks were placed in Transwell Inserts, providing indirect contact with MC3T3-E1 cells. Succinate dehydrogenase activity of the cells was evaluated over a 6-week period using MTT ((3-(4,5-Dimethylthiazol-2- yl)-2,5-diphenyltetrazolium bromide) assay. Cytotoxicity profiles of BC Sealer and AH Plus were fitted with polynomial regression models. The time for 50% of the cells to survive (T0.5) was analyzed using the Wald statistic with a two-tailed significance level of 0.05. Results: BC Sealer required at least 168 hours to reach the final setting using the Gilmore needle method, and its microhardeness significantly declined when water was included in the sealer (P = .004). All set sealers exhibited severe cytotoxicity at 24 hours. The cytotoxicity of AH Plus gradually decreased and became noncytotoxic, whereas BC Sealer remained moderately cytotoxic over the 6-week period. A significant difference (P < .001) was detected between T0.5 of BC Sealer (5.10 weeks; 95% confidence interval [CI], 4.69-5.42, standard error [SE] = 0.09) and T0.5 of AH Plus (0.86 weeks; 95% CI, 0.68-1.05; SE = 0.18). Conclusions: Further studies are required to evaluate the correlation between the length of setting time of BC Sealer and its degree of cytotoxicity.

Original languageEnglish (US)
Pages (from-to)673-677
Number of pages5
JournalJournal of Endodontics
Volume37
Issue number5
DOIs
StatePublished - May 1 2011

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Dental Pulp Cavity
Confidence Intervals
Epoxy Resins
Silicates
Succinate Dehydrogenase
Polytetrafluoroethylene
Statistical Models
Lakes
Needles
epoxy resin-based root canal sealer
canals sealer
Water
Sealer Plus

Keywords

  • Biocompatibility
  • MTT
  • microhardness
  • premixed calcium silicate-based sealer
  • setting time

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Setting properties and cytotoxicity evaluation of a premixed bioceramic root canal sealer. / Loushine, Bethany A.; Bryan, Thomas E.; Looney, Stephen Warwick; Gillen, Brian M.; Loushine, Robert J.; Weller, R. Norman; Pashley, David Henry; Tay, Franklin Chi Meng.

In: Journal of Endodontics, Vol. 37, No. 5, 01.05.2011, p. 673-677.

Research output: Contribution to journalArticle

Loushine, Bethany A. ; Bryan, Thomas E. ; Looney, Stephen Warwick ; Gillen, Brian M. ; Loushine, Robert J. ; Weller, R. Norman ; Pashley, David Henry ; Tay, Franklin Chi Meng. / Setting properties and cytotoxicity evaluation of a premixed bioceramic root canal sealer. In: Journal of Endodontics. 2011 ; Vol. 37, No. 5. pp. 673-677.
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abstract = "Introduction: This study investigated the setting time and micohardness of a premixed calcium phosphate silicate-based sealer (EndoSequence BC Sealer; Brasseler USA, Savannah, GA) in the presence of different moisture contents (0-9 wt{\%}). The moisture content that produced the most optimal setting properties was used to prepare set EndoSequence BC Sealer for cytotoxicity comparison with an epoxy resin-based sealer (AH Plus; Dentsply Caulk, Milford, DE). Methods: Standardized disks were created with BC Sealer, AH Plus, Pulp Canal Sealer EWT (positive control) (SybronEndo, Orange CA), and Teflon (Small Parts Inc., Miami Lakes, FL; negative control). Disks were placed in Transwell Inserts, providing indirect contact with MC3T3-E1 cells. Succinate dehydrogenase activity of the cells was evaluated over a 6-week period using MTT ((3-(4,5-Dimethylthiazol-2- yl)-2,5-diphenyltetrazolium bromide) assay. Cytotoxicity profiles of BC Sealer and AH Plus were fitted with polynomial regression models. The time for 50{\%} of the cells to survive (T0.5) was analyzed using the Wald statistic with a two-tailed significance level of 0.05. Results: BC Sealer required at least 168 hours to reach the final setting using the Gilmore needle method, and its microhardeness significantly declined when water was included in the sealer (P = .004). All set sealers exhibited severe cytotoxicity at 24 hours. The cytotoxicity of AH Plus gradually decreased and became noncytotoxic, whereas BC Sealer remained moderately cytotoxic over the 6-week period. A significant difference (P < .001) was detected between T0.5 of BC Sealer (5.10 weeks; 95{\%} confidence interval [CI], 4.69-5.42, standard error [SE] = 0.09) and T0.5 of AH Plus (0.86 weeks; 95{\%} CI, 0.68-1.05; SE = 0.18). Conclusions: Further studies are required to evaluate the correlation between the length of setting time of BC Sealer and its degree of cytotoxicity.",
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AU - Looney, Stephen Warwick

AU - Gillen, Brian M.

AU - Loushine, Robert J.

AU - Weller, R. Norman

AU - Pashley, David Henry

AU - Tay, Franklin Chi Meng

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N2 - Introduction: This study investigated the setting time and micohardness of a premixed calcium phosphate silicate-based sealer (EndoSequence BC Sealer; Brasseler USA, Savannah, GA) in the presence of different moisture contents (0-9 wt%). The moisture content that produced the most optimal setting properties was used to prepare set EndoSequence BC Sealer for cytotoxicity comparison with an epoxy resin-based sealer (AH Plus; Dentsply Caulk, Milford, DE). Methods: Standardized disks were created with BC Sealer, AH Plus, Pulp Canal Sealer EWT (positive control) (SybronEndo, Orange CA), and Teflon (Small Parts Inc., Miami Lakes, FL; negative control). Disks were placed in Transwell Inserts, providing indirect contact with MC3T3-E1 cells. Succinate dehydrogenase activity of the cells was evaluated over a 6-week period using MTT ((3-(4,5-Dimethylthiazol-2- yl)-2,5-diphenyltetrazolium bromide) assay. Cytotoxicity profiles of BC Sealer and AH Plus were fitted with polynomial regression models. The time for 50% of the cells to survive (T0.5) was analyzed using the Wald statistic with a two-tailed significance level of 0.05. Results: BC Sealer required at least 168 hours to reach the final setting using the Gilmore needle method, and its microhardeness significantly declined when water was included in the sealer (P = .004). All set sealers exhibited severe cytotoxicity at 24 hours. The cytotoxicity of AH Plus gradually decreased and became noncytotoxic, whereas BC Sealer remained moderately cytotoxic over the 6-week period. A significant difference (P < .001) was detected between T0.5 of BC Sealer (5.10 weeks; 95% confidence interval [CI], 4.69-5.42, standard error [SE] = 0.09) and T0.5 of AH Plus (0.86 weeks; 95% CI, 0.68-1.05; SE = 0.18). Conclusions: Further studies are required to evaluate the correlation between the length of setting time of BC Sealer and its degree of cytotoxicity.

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