Small heat shock proteins and vasospasm in human umbilical artery smooth muscle

Colleen M. Brophy, Arthur Beall, Shannon Lamb, Mary Dickinson, Debra J Ware

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Human umbilical artery smooth muscle is uniquely refractory to cyclic nucleotide-dependent vasorelaxation. Small heat shock proteins (HSPs) have been implicated as contractile regulatory proteins. Thus, we hypothesized that alterations in the phosphorylation of small HSPs may contribute to human umbilical artery smooth muscle vasospasm. Physiologic contractile responses were determined in a muscle bath and compared with phosphorylation events determined with whole-cell phosphorylation and 2-dimensional gel electrophoresis. Precontraction of bovine carotid artery Smooth muscle with serotonin followed by relaxation with forskolin was associated with increases in the phosphorylation of HSP27 and HSP20. Precontraction of umbilical artery with serotonin followed by forskolin treatment led to increases in the phosphorylation of HSP27. However, the umbilical artery smooth muscle did not relax, nor was there an increase in the phosphorylation of HSP20 with forskolin treatment. These data suggest that impaired cyclic nucleotide- dependent relaxation of umbilical artery smooth muscle is associated with a lack of phosphorylation of HSP20.

Original languageEnglish (US)
Pages (from-to)1354-1359
Number of pages6
JournalBiology of Reproduction
Volume57
Issue number6
DOIs
StatePublished - Jan 1 1997

Fingerprint

Small Heat-Shock Proteins
Umbilical Arteries
Smooth Muscle
Phosphorylation
Colforsin
Cyclic Nucleotides
Serotonin
Contractile Proteins
Baths
Carotid Arteries
Vasodilation
Electrophoresis
Gels
Muscles
Therapeutics

ASJC Scopus subject areas

  • Cell Biology

Cite this

Small heat shock proteins and vasospasm in human umbilical artery smooth muscle. / Brophy, Colleen M.; Beall, Arthur; Lamb, Shannon; Dickinson, Mary; Ware, Debra J.

In: Biology of Reproduction, Vol. 57, No. 6, 01.01.1997, p. 1354-1359.

Research output: Contribution to journalArticle

Brophy, CM, Beall, A, Lamb, S, Dickinson, M & Ware, DJ 1997, 'Small heat shock proteins and vasospasm in human umbilical artery smooth muscle', Biology of Reproduction, vol. 57, no. 6, pp. 1354-1359. https://doi.org/10.1095/biolreprod57.6.1354
Brophy, Colleen M. ; Beall, Arthur ; Lamb, Shannon ; Dickinson, Mary ; Ware, Debra J. / Small heat shock proteins and vasospasm in human umbilical artery smooth muscle. In: Biology of Reproduction. 1997 ; Vol. 57, No. 6. pp. 1354-1359.
@article{07477a2cad1d4246910a8a4528c7f773,
title = "Small heat shock proteins and vasospasm in human umbilical artery smooth muscle",
abstract = "Human umbilical artery smooth muscle is uniquely refractory to cyclic nucleotide-dependent vasorelaxation. Small heat shock proteins (HSPs) have been implicated as contractile regulatory proteins. Thus, we hypothesized that alterations in the phosphorylation of small HSPs may contribute to human umbilical artery smooth muscle vasospasm. Physiologic contractile responses were determined in a muscle bath and compared with phosphorylation events determined with whole-cell phosphorylation and 2-dimensional gel electrophoresis. Precontraction of bovine carotid artery Smooth muscle with serotonin followed by relaxation with forskolin was associated with increases in the phosphorylation of HSP27 and HSP20. Precontraction of umbilical artery with serotonin followed by forskolin treatment led to increases in the phosphorylation of HSP27. However, the umbilical artery smooth muscle did not relax, nor was there an increase in the phosphorylation of HSP20 with forskolin treatment. These data suggest that impaired cyclic nucleotide- dependent relaxation of umbilical artery smooth muscle is associated with a lack of phosphorylation of HSP20.",
author = "Brophy, {Colleen M.} and Arthur Beall and Shannon Lamb and Mary Dickinson and Ware, {Debra J}",
year = "1997",
month = "1",
day = "1",
doi = "10.1095/biolreprod57.6.1354",
language = "English (US)",
volume = "57",
pages = "1354--1359",
journal = "Biology of Reproduction",
issn = "0006-3363",
publisher = "Society for the Study of Reproduction",
number = "6",

}

TY - JOUR

T1 - Small heat shock proteins and vasospasm in human umbilical artery smooth muscle

AU - Brophy, Colleen M.

AU - Beall, Arthur

AU - Lamb, Shannon

AU - Dickinson, Mary

AU - Ware, Debra J

PY - 1997/1/1

Y1 - 1997/1/1

N2 - Human umbilical artery smooth muscle is uniquely refractory to cyclic nucleotide-dependent vasorelaxation. Small heat shock proteins (HSPs) have been implicated as contractile regulatory proteins. Thus, we hypothesized that alterations in the phosphorylation of small HSPs may contribute to human umbilical artery smooth muscle vasospasm. Physiologic contractile responses were determined in a muscle bath and compared with phosphorylation events determined with whole-cell phosphorylation and 2-dimensional gel electrophoresis. Precontraction of bovine carotid artery Smooth muscle with serotonin followed by relaxation with forskolin was associated with increases in the phosphorylation of HSP27 and HSP20. Precontraction of umbilical artery with serotonin followed by forskolin treatment led to increases in the phosphorylation of HSP27. However, the umbilical artery smooth muscle did not relax, nor was there an increase in the phosphorylation of HSP20 with forskolin treatment. These data suggest that impaired cyclic nucleotide- dependent relaxation of umbilical artery smooth muscle is associated with a lack of phosphorylation of HSP20.

AB - Human umbilical artery smooth muscle is uniquely refractory to cyclic nucleotide-dependent vasorelaxation. Small heat shock proteins (HSPs) have been implicated as contractile regulatory proteins. Thus, we hypothesized that alterations in the phosphorylation of small HSPs may contribute to human umbilical artery smooth muscle vasospasm. Physiologic contractile responses were determined in a muscle bath and compared with phosphorylation events determined with whole-cell phosphorylation and 2-dimensional gel electrophoresis. Precontraction of bovine carotid artery Smooth muscle with serotonin followed by relaxation with forskolin was associated with increases in the phosphorylation of HSP27 and HSP20. Precontraction of umbilical artery with serotonin followed by forskolin treatment led to increases in the phosphorylation of HSP27. However, the umbilical artery smooth muscle did not relax, nor was there an increase in the phosphorylation of HSP20 with forskolin treatment. These data suggest that impaired cyclic nucleotide- dependent relaxation of umbilical artery smooth muscle is associated with a lack of phosphorylation of HSP20.

UR - http://www.scopus.com/inward/record.url?scp=0030712228&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030712228&partnerID=8YFLogxK

U2 - 10.1095/biolreprod57.6.1354

DO - 10.1095/biolreprod57.6.1354

M3 - Article

C2 - 9408240

AN - SCOPUS:0030712228

VL - 57

SP - 1354

EP - 1359

JO - Biology of Reproduction

JF - Biology of Reproduction

SN - 0006-3363

IS - 6

ER -