Somatic mutation in genes for the variable portion of the immunoglobulin heavy chain

John Sims, Terence H. Rabbitts, Pila Estess, Clive A. Slaughter, Philip W. Tucker, J. Donald Capra

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The size of the gene pool potentially encoding antibodies to p-azophenyl arsenate has been examined. A heavy chain-specific full-length complementary DNA clone has been constructed with the use of messenger RNA from a hybridoma that produces antibodies to the arsenate hapten and bears nearly a full complement of the determinants comprising the cross-reactive idiotype (CRI). The sequences of both the complementary DNA clone and the corresponding immunoglobulin heavy chain have been independently determined. A probe for the variable region gene was prepared from the original heavy chain complementary DNA clone and used to analyze, by Southern filter hybridization, genomic DNA from both A/J (CRI positive) and BALB/c (CRI negative) mice. Approximately 20 to 25 restriction fragments containing "germline" variable region gene segments were detected in both strains, and many are shared by both. Since 35 CRI-positive heavy chains have been partially sequenced thus far and 31 are different, the results of the hybridization analysis suggest that somatic mutation events involving the variable region gene segments of the heavy chain play a role in the origin of the amino acid sequence diversity seen in this system.

Original languageEnglish (US)
Pages (from-to)309-311
Number of pages3
JournalScience
Volume216
Issue number4543
DOIs
StatePublished - Jan 1 1982
Externally publishedYes

Fingerprint

Immunoglobulin Heavy Chains
Complementary DNA
Clone Cells
Mutation
Genes
Nucleic Acid Hybridization
Gene Pool
Antibodies
Haptens
Hybridomas
Amino Acid Sequence
Messenger RNA
DNA
arsenic acid

ASJC Scopus subject areas

  • General

Cite this

Sims, J., Rabbitts, T. H., Estess, P., Slaughter, C. A., Tucker, P. W., & Capra, J. D. (1982). Somatic mutation in genes for the variable portion of the immunoglobulin heavy chain. Science, 216(4543), 309-311. https://doi.org/10.1126/science.6801765

Somatic mutation in genes for the variable portion of the immunoglobulin heavy chain. / Sims, John; Rabbitts, Terence H.; Estess, Pila; Slaughter, Clive A.; Tucker, Philip W.; Capra, J. Donald.

In: Science, Vol. 216, No. 4543, 01.01.1982, p. 309-311.

Research output: Contribution to journalArticle

Sims, J, Rabbitts, TH, Estess, P, Slaughter, CA, Tucker, PW & Capra, JD 1982, 'Somatic mutation in genes for the variable portion of the immunoglobulin heavy chain', Science, vol. 216, no. 4543, pp. 309-311. https://doi.org/10.1126/science.6801765
Sims, John ; Rabbitts, Terence H. ; Estess, Pila ; Slaughter, Clive A. ; Tucker, Philip W. ; Capra, J. Donald. / Somatic mutation in genes for the variable portion of the immunoglobulin heavy chain. In: Science. 1982 ; Vol. 216, No. 4543. pp. 309-311.
@article{4139a99d77094cd2a5b5633c8271b4ff,
title = "Somatic mutation in genes for the variable portion of the immunoglobulin heavy chain",
abstract = "The size of the gene pool potentially encoding antibodies to p-azophenyl arsenate has been examined. A heavy chain-specific full-length complementary DNA clone has been constructed with the use of messenger RNA from a hybridoma that produces antibodies to the arsenate hapten and bears nearly a full complement of the determinants comprising the cross-reactive idiotype (CRI). The sequences of both the complementary DNA clone and the corresponding immunoglobulin heavy chain have been independently determined. A probe for the variable region gene was prepared from the original heavy chain complementary DNA clone and used to analyze, by Southern filter hybridization, genomic DNA from both A/J (CRI positive) and BALB/c (CRI negative) mice. Approximately 20 to 25 restriction fragments containing {"}germline{"} variable region gene segments were detected in both strains, and many are shared by both. Since 35 CRI-positive heavy chains have been partially sequenced thus far and 31 are different, the results of the hybridization analysis suggest that somatic mutation events involving the variable region gene segments of the heavy chain play a role in the origin of the amino acid sequence diversity seen in this system.",
author = "John Sims and Rabbitts, {Terence H.} and Pila Estess and Slaughter, {Clive A.} and Tucker, {Philip W.} and Capra, {J. Donald}",
year = "1982",
month = "1",
day = "1",
doi = "10.1126/science.6801765",
language = "English (US)",
volume = "216",
pages = "309--311",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "4543",

}

TY - JOUR

T1 - Somatic mutation in genes for the variable portion of the immunoglobulin heavy chain

AU - Sims, John

AU - Rabbitts, Terence H.

AU - Estess, Pila

AU - Slaughter, Clive A.

AU - Tucker, Philip W.

AU - Capra, J. Donald

PY - 1982/1/1

Y1 - 1982/1/1

N2 - The size of the gene pool potentially encoding antibodies to p-azophenyl arsenate has been examined. A heavy chain-specific full-length complementary DNA clone has been constructed with the use of messenger RNA from a hybridoma that produces antibodies to the arsenate hapten and bears nearly a full complement of the determinants comprising the cross-reactive idiotype (CRI). The sequences of both the complementary DNA clone and the corresponding immunoglobulin heavy chain have been independently determined. A probe for the variable region gene was prepared from the original heavy chain complementary DNA clone and used to analyze, by Southern filter hybridization, genomic DNA from both A/J (CRI positive) and BALB/c (CRI negative) mice. Approximately 20 to 25 restriction fragments containing "germline" variable region gene segments were detected in both strains, and many are shared by both. Since 35 CRI-positive heavy chains have been partially sequenced thus far and 31 are different, the results of the hybridization analysis suggest that somatic mutation events involving the variable region gene segments of the heavy chain play a role in the origin of the amino acid sequence diversity seen in this system.

AB - The size of the gene pool potentially encoding antibodies to p-azophenyl arsenate has been examined. A heavy chain-specific full-length complementary DNA clone has been constructed with the use of messenger RNA from a hybridoma that produces antibodies to the arsenate hapten and bears nearly a full complement of the determinants comprising the cross-reactive idiotype (CRI). The sequences of both the complementary DNA clone and the corresponding immunoglobulin heavy chain have been independently determined. A probe for the variable region gene was prepared from the original heavy chain complementary DNA clone and used to analyze, by Southern filter hybridization, genomic DNA from both A/J (CRI positive) and BALB/c (CRI negative) mice. Approximately 20 to 25 restriction fragments containing "germline" variable region gene segments were detected in both strains, and many are shared by both. Since 35 CRI-positive heavy chains have been partially sequenced thus far and 31 are different, the results of the hybridization analysis suggest that somatic mutation events involving the variable region gene segments of the heavy chain play a role in the origin of the amino acid sequence diversity seen in this system.

UR - http://www.scopus.com/inward/record.url?scp=0020035833&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020035833&partnerID=8YFLogxK

U2 - 10.1126/science.6801765

DO - 10.1126/science.6801765

M3 - Article

VL - 216

SP - 309

EP - 311

JO - Science

JF - Science

SN - 0036-8075

IS - 4543

ER -