Specificity and functional characteristics of anti-HLA-A mAbs LGIII-147.4. 1 and LGIII-220.6.2

X. Wang, B. Liang, V. Rebmann, J. Lu, Esteban Celis, T. Kageshita, H. Grosse-Wilde, Soldano Ferrone

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Only three anti-HLA-A monoclonal antibodies (mAbs) have been described in the literature. Two of them recognize determinants shared by only a few HLA-A allospecificities. The third one, mAb 3G11, recognizes a determinant shared by most HLA-A allospecificities. Being an IgM, the latter mAb is not likely to be a useful probe in immunohistochemical reactions and in functional assays. Therefore, in the present study we have characterized the specificity of the mAbs LGIII-147.4.1 and LGIII-220.6.2. The two mAbs that do not share idiotypic determinants recognize distinct but spatially close antigenic determinants expressed on most of the gene products of the HLA-A locus. Specifically, the determinant recognized by mAb LGIII-220.6.2 is expressed on HLA-A1, -A2, -A3, -A26, -A28, -A29, -A30, -A33, -A36, -A74 and -A80 allospecificities. The determinant recognized by mAb LGIII-147.4.1, which appears to be located on the amino-add residues 79-83 of the heavy chain, is expressed on all HLA-A allospecificities but HLA-A23, -A24, -A25 and -A32. Because of its broad reactivity, the mAb LGIII-147.4.1 was characterized in a number of assays. It was found to be a useful probe to measure the HLA-A antigen level in serum, to assess the HLA. A restriction of cytotoxic T lymphocytes (CTL) and to monitor HLA-A antigen expression in normal and malignant lesions.

Original languageEnglish (US)
Pages (from-to)139-148
Number of pages10
JournalTissue Antigens
Volume62
Issue number2
DOIs
StatePublished - Aug 1 2003

Keywords

  • Cell-mediated cytotoxicity
  • HLA-A antigens
  • Immunohistochemistry
  • Monoclonal antibodies
  • Serum HLA-A antigens

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Genetics

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    Wang, X., Liang, B., Rebmann, V., Lu, J., Celis, E., Kageshita, T., Grosse-Wilde, H., & Ferrone, S. (2003). Specificity and functional characteristics of anti-HLA-A mAbs LGIII-147.4. 1 and LGIII-220.6.2. Tissue Antigens, 62(2), 139-148. https://doi.org/10.1034/j.1399-0039.2003.00087.x