Streamlined subclass-specific absolute quantification of serum igg glycopeptides using synthetic isotope-labeled standards

Lei Li, Shuaishuai Wang, Ding Liu, Jingyao Qu, He Zhu, Congcong Chen, Christopher Gibbons, Harmon Greenway, Peng Wang, Roni J. Bollag, Kebin Liu

Research output: Contribution to journalArticlepeer-review

Abstract

Absolute glycoproteomics quantification has drawn tremendous attention owing to its prospects in biomarker discovery and clinical implementation but is impeded by a general lack of suitable heavy isotope-labeled glycopeptide standards. In this study, we devised a facile chemoenzymatic strategy to synthesize a total of 36 human IgG glycopeptides attached with well-defined glycoforms, including 15 isotope-labeled ones with a mass increment of 6 Da to their native counterparts. Spiking of these standards into human sera enabled simplified, robust, and precise absolute quantification of IgG glycopeptides in a subclass-specific fashion. Additionally, the implementation of the absolute quantification approach revealed subclass-dependent alteration of serum IgG galactosylation and sialylation in colon cancer samples.

Original languageEnglish (US)
Pages (from-to)4449-4455
Number of pages7
JournalAnalytical Chemistry
Volume93
Issue number10
DOIs
StatePublished - Mar 16 2021

ASJC Scopus subject areas

  • Analytical Chemistry

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