Structural characterization of a Lymnaea putative endoprotease related to human furin

August B. Smit, Sabine Spijker, Gregg Thomas Nagle, Susan L. Knock, Alexander Kurosky, Wijnand P.M. Geraerts

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A number of peptides have been identified in the central nervous system of the freshwater snail, Lymnaea stagnalis, that function as hormones and neurotransmitters/neuromodulators. These peptides are typically proteolytically processed from larger prohormones mostly at sites composed of single or multiple basic amino acid residues. Previously we demonstrated a diversity of putative prohormone convertases that may be involved in prohormone processing in the Lymnaea brain. In the present report, we have characterized a cDNA clone encoding a putative endoprotease of 837 amino acids. The primary structure of the endoprotease (Lfur2) was comparable to that of human furin and contained a putative catalytic domain, a Cys-rich domain, and a transmembrane region. The catalytic domain of Lfur2 demonstrated about 70% residue identity when compared with human furin, PACE4 and Drosophila Dfur1 and dKLIP-1. The Lfur2 gene was expressed in the central nervous system as well as various peripheral tissues of Lymnaea.

Original languageEnglish (US)
Pages (from-to)27-31
Number of pages5
JournalFEBS Letters
Volume343
Issue number1
DOIs
StatePublished - Apr 18 1994

Fingerprint

Furin
Lymnaea
Neurology
Neurotransmitter Agents
Catalytic Domain
Central Nervous System
Proprotein Convertases
Basic Amino Acids
Peptides
Snails
Fresh Water
Drosophila
Brain
Complementary DNA
Clone Cells
Genes
Hormones
Tissue
Amino Acids
Processing

Keywords

  • Central nervous system
  • Furin-related endoprotease
  • Lymnaeastagnalis
  • Mollusc
  • Polymerase chain reaction
  • cDNA cloning

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Structural characterization of a Lymnaea putative endoprotease related to human furin. / Smit, August B.; Spijker, Sabine; Nagle, Gregg Thomas; Knock, Susan L.; Kurosky, Alexander; Geraerts, Wijnand P.M.

In: FEBS Letters, Vol. 343, No. 1, 18.04.1994, p. 27-31.

Research output: Contribution to journalArticle

Smit, August B. ; Spijker, Sabine ; Nagle, Gregg Thomas ; Knock, Susan L. ; Kurosky, Alexander ; Geraerts, Wijnand P.M. / Structural characterization of a Lymnaea putative endoprotease related to human furin. In: FEBS Letters. 1994 ; Vol. 343, No. 1. pp. 27-31.
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