Structural Features of Transcription Factor IIIA Bound to a Nucleosome in Solution

Joseph M Vitolo, Zungyoon Yang, Ravi Basavappa, Jeffrey J. Hayes

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Assembly of a DNA fragment containing a Xenopus borealis somatic-type 5S RNA gene into a nucleosome greatly restricts binding of the 5S gene-specific transcription factor IIIA (TFIIIA) to the 5S internal promoter. However, TFIIIA binds with high affinity to 5S nucleosomes lacking the N-terminal tail domains of the core histones or to nucleosomes in which these domains are hyperacetylated. The degree to which tail acetylation or removal improves TFIIIA binding cannot be simply explained by a commensurate change in the general accessibility of nucleosomal DNA. In order to investigate the molecular basis of how TFIIIA binds to the nucleosome and to ascertain if binding involves all nine zinc fingers and/or displacement of histone-DNA interactions, we examined the TFIIIA-nucleosome complex by hydroxyl radical footprinting and site-directed protein-DNA cross-linking. Our data reveal that the first six fingers of TFIIIA bind and displace approximately 20 bp of histone-DNA interactions at the periphery of the nucleosome, while binding of fingers 7 to 9 appears to overlap with histone-DNA interactions. Molecular modeling based on these results and the crystal structures of a nucleosome core and a TFIIIA-DNA cocomplex yields a precise picture of the ternary complex and a potentially important intermediate in the transition from naive chromatin structure to productive polymerase III transcription complex.

Original languageEnglish (US)
Pages (from-to)697-707
Number of pages11
JournalMolecular and Cellular Biology
Volume24
Issue number2
DOIs
StatePublished - Jan 1 2004

Fingerprint

Transcription Factor TFIIIA
Nucleosomes
DNA
Histones
Fingers
Tail
Zinc Fingers
Acetylation
Xenopus
Hydroxyl Radical
Chromatin
RNA

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Structural Features of Transcription Factor IIIA Bound to a Nucleosome in Solution. / Vitolo, Joseph M; Yang, Zungyoon; Basavappa, Ravi; Hayes, Jeffrey J.

In: Molecular and Cellular Biology, Vol. 24, No. 2, 01.01.2004, p. 697-707.

Research output: Contribution to journalArticle

Vitolo, Joseph M ; Yang, Zungyoon ; Basavappa, Ravi ; Hayes, Jeffrey J. / Structural Features of Transcription Factor IIIA Bound to a Nucleosome in Solution. In: Molecular and Cellular Biology. 2004 ; Vol. 24, No. 2. pp. 697-707.
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