Study on the anti-apoptosis effect of insulin-like growth factor I on the regulation of neural precursor cells survival

Mei Tang, Qi Wang, He Dong Li, Fan Yang

Research output: Contribution to journalArticlepeer-review

Abstract

Objective To investigate the effect of insulin-like growth factor I (IGF- I ) on the anti-apoptosis of neural precursor cells. Methods Rat neural precursor cells (Ge6) were used as experiment object. 10 ng/ml. IGF-I were applied to cultured Ge6 differentiated 2d, 4 d, 6 d and the untreated cells as control. Quantitative reverse transcriptase PCR (qRT-PCR) was used to detect the effect of the IGF- I on caspase-3- mRNA in the Ge6 of different groups and Immunofluorescent staining were applied to obverse the positive rate of caspase-3. Results qRT-PCR demonstrated that caspase-3 mRNA level of experimental group decreased compared to that of the control group, the differences between experimental group cultured for 4 d (2. 007±0. 297), 6 d (1. 194±0. 244) and the control group cultured for 4 d (5. 530± 1. 027), 6 d (5. 371±0. 513) were statistically significant (P<0 05). Immunofluorescent staining indicated that the caspase-3 positive rate of experimental group cultured for 2 d, 4 d and 6 d were reduced compared to that of the control group, the differences between experimental group cultured for 4 d (3.5%±0. 2%), 6 d (5. 1% ±0. 1%) and the control group cultured for 4d(7.0%±0. 5%), 6d(7.4%±0. 4%) were statistically significant (P<0 05). Conclusion IGF-I plays an anti-apoptosis role on neural precursor cells.

Original languageEnglish (US)
Pages (from-to)15-18
Number of pages4
JournalJournal of Sichuan University (Medical Science Edition)
Volume45
Issue number1
StatePublished - Jan 2014
Externally publishedYes

Keywords

  • Apoptosis caspase-3
  • Insulin-like growth factor i
  • Neural precursor cells

ASJC Scopus subject areas

  • Molecular Biology

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