Abstract
To get a long-term culture of tumor-infiltrating lymphocytes (TILs) is very difficult. The authors have investigated some suitable enzymes, their digestive conditions such as time and temperature, which may influence the viability and cytotoxicity of TILs. The results showed that collagenase II and IV could keep viability of TILs much longer than those treated with trypsin or hyaluronidase. The digestion with collagenase II or IV at 4 degrees C for 24 hours was much less damage to viability of TILs than those treated at 37 degrees C for one hour. The TILs, which digested at 4 degrees C for 24 hours, still had cytotoxicity against autologous tumor cells as long as sixty to seventy-five days.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 103-107 |
| Number of pages | 5 |
| Journal | Shi yan sheng wu xue bao = Journal of experimental biology |
| Volume | 27 |
| Issue number | 1 |
| State | Published - Mar 1 1994 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Medicine(all)
Cite this
Study on the influence of enzymatic digestion upon tumor-infiltrating lymphocytes. / Li, Biaoru; Tong, S. Q.; Hu, B. Y.; Zhu, Y. M.; Zhang, X. H.; Wu, J. H.; Lu, J.; Lu, D. Y.
In: Shi yan sheng wu xue bao = Journal of experimental biology, Vol. 27, No. 1, 01.03.1994, p. 103-107.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Study on the influence of enzymatic digestion upon tumor-infiltrating lymphocytes
AU - Li, Biaoru
AU - Tong, S. Q.
AU - Hu, B. Y.
AU - Zhu, Y. M.
AU - Zhang, X. H.
AU - Wu, J. H.
AU - Lu, J.
AU - Lu, D. Y.
PY - 1994/3/1
Y1 - 1994/3/1
N2 - To get a long-term culture of tumor-infiltrating lymphocytes (TILs) is very difficult. The authors have investigated some suitable enzymes, their digestive conditions such as time and temperature, which may influence the viability and cytotoxicity of TILs. The results showed that collagenase II and IV could keep viability of TILs much longer than those treated with trypsin or hyaluronidase. The digestion with collagenase II or IV at 4 degrees C for 24 hours was much less damage to viability of TILs than those treated at 37 degrees C for one hour. The TILs, which digested at 4 degrees C for 24 hours, still had cytotoxicity against autologous tumor cells as long as sixty to seventy-five days.
AB - To get a long-term culture of tumor-infiltrating lymphocytes (TILs) is very difficult. The authors have investigated some suitable enzymes, their digestive conditions such as time and temperature, which may influence the viability and cytotoxicity of TILs. The results showed that collagenase II and IV could keep viability of TILs much longer than those treated with trypsin or hyaluronidase. The digestion with collagenase II or IV at 4 degrees C for 24 hours was much less damage to viability of TILs than those treated at 37 degrees C for one hour. The TILs, which digested at 4 degrees C for 24 hours, still had cytotoxicity against autologous tumor cells as long as sixty to seventy-five days.
UR - http://www.scopus.com/inward/record.url?scp=0028391056&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028391056&partnerID=8YFLogxK
M3 - Article
C2 - 8042406
AN - SCOPUS:0028391056
VL - 27
SP - 103
EP - 107
JO - Journal of Molecular Cell Biology
JF - Journal of Molecular Cell Biology
SN - 1674-2788
IS - 1
ER -