Study on the influence of enzymatic digestion upon tumor-infiltrating lymphocytes

Biaoru Li; S. Q. Tong; B. Y. Hu; Y. M. Zhu; X. H. Zhang; J. H. Wu; J. Lu; D. Y. Lu

Study on the influence of enzymatic digestion upon tumor-infiltrating lymphocytes

Biaoru Li, S. Q. Tong, B. Y. Hu, Y. M. Zhu, X. H. Zhang, J. H. Wu, J. Lu, D. Y. Lu

Research output: Contribution to journal › Article › peer-review

Abstract

To get a long-term culture of tumor-infiltrating lymphocytes (TILs) is very difficult. The authors have investigated some suitable enzymes, their digestive conditions such as time and temperature, which may influence the viability and cytotoxicity of TILs. The results showed that collagenase II and IV could keep viability of TILs much longer than those treated with trypsin or hyaluronidase. The digestion with collagenase II or IV at 4 degrees C for 24 hours was much less damage to viability of TILs than those treated at 37 degrees C for one hour. The TILs, which digested at 4 degrees C for 24 hours, still had cytotoxicity against autologous tumor cells as long as sixty to seventy-five days.

Original language	English (US)
Pages (from-to)	103-107
Number of pages	5
Journal	Shi yan sheng wu xue bao = Journal of experimental biology
Volume	27
Issue number	1
State	Published - Mar 1 1994
Externally published	Yes

ASJC Scopus subject areas

Medicine(all)

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title = "Study on the influence of enzymatic digestion upon tumor-infiltrating lymphocytes",

abstract = "To get a long-term culture of tumor-infiltrating lymphocytes (TILs) is very difficult. The authors have investigated some suitable enzymes, their digestive conditions such as time and temperature, which may influence the viability and cytotoxicity of TILs. The results showed that collagenase II and IV could keep viability of TILs much longer than those treated with trypsin or hyaluronidase. The digestion with collagenase II or IV at 4 degrees C for 24 hours was much less damage to viability of TILs than those treated at 37 degrees C for one hour. The TILs, which digested at 4 degrees C for 24 hours, still had cytotoxicity against autologous tumor cells as long as sixty to seventy-five days.",

author = "Biaoru Li and Tong, {S. Q.} and Hu, {B. Y.} and Zhu, {Y. M.} and Zhang, {X. H.} and Wu, {J. H.} and J. Lu and Lu, {D. Y.}",

year = "1994",

month = mar,

day = "1",

language = "English (US)",

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journal = "Journal of Molecular Cell Biology",

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AU - Li, Biaoru

AU - Tong, S. Q.

AU - Hu, B. Y.

AU - Zhu, Y. M.

AU - Zhang, X. H.

AU - Wu, J. H.

AU - Lu, J.

AU - Lu, D. Y.

PY - 1994/3/1

Y1 - 1994/3/1

N2 - To get a long-term culture of tumor-infiltrating lymphocytes (TILs) is very difficult. The authors have investigated some suitable enzymes, their digestive conditions such as time and temperature, which may influence the viability and cytotoxicity of TILs. The results showed that collagenase II and IV could keep viability of TILs much longer than those treated with trypsin or hyaluronidase. The digestion with collagenase II or IV at 4 degrees C for 24 hours was much less damage to viability of TILs than those treated at 37 degrees C for one hour. The TILs, which digested at 4 degrees C for 24 hours, still had cytotoxicity against autologous tumor cells as long as sixty to seventy-five days.

AB - To get a long-term culture of tumor-infiltrating lymphocytes (TILs) is very difficult. The authors have investigated some suitable enzymes, their digestive conditions such as time and temperature, which may influence the viability and cytotoxicity of TILs. The results showed that collagenase II and IV could keep viability of TILs much longer than those treated with trypsin or hyaluronidase. The digestion with collagenase II or IV at 4 degrees C for 24 hours was much less damage to viability of TILs than those treated at 37 degrees C for one hour. The TILs, which digested at 4 degrees C for 24 hours, still had cytotoxicity against autologous tumor cells as long as sixty to seventy-five days.

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Study on the influence of enzymatic digestion upon tumor-infiltrating lymphocytes

Abstract

ASJC Scopus subject areas

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