Abstract
Synchrotron radiation is an excellent excitation source for both the time-domain (pulse) and the frequency-domain (phase/modulation) methods of time-resolved fluorescence spectroscopy. All possible wavelengths are available to excite fluorescent biological probes since synchrotron radiation consists of an intense continuum of energies from the IR to X-rays. The pulsed nature of synchrotron radiation permits time-correlated single photoncounting techniques to collect a histogram of the probability of the decay of the excited state. The harmonics of the pulsed exciting light also provide a wide range of modulation frequencies that permit the frequency-domain method to match the current resolution for time-domain measurements.
Original language | English (US) |
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Pages (from-to) | 29-33 |
Number of pages | 5 |
Journal | Proceedings of SPIE - The International Society for Optical Engineering |
Volume | 909 |
DOIs | |
State | Published - Jun 24 1988 |
Externally published | Yes |
Event | Time-Resolved Laser Spectroscopy in Biochemistry 1988 - Los Angeles, United States Duration: Jan 11 1988 → Jan 17 1988 |
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Computer Science Applications
- Applied Mathematics
- Electrical and Electronic Engineering