Synchrotron radiation as an excitation source in time-domain and frequency-domain fluorometry

William R. Laws, John Clark Sutherland

Research output: Contribution to journalConference article

Abstract

Synchrotron radiation is an excellent excitation source for both the time-domain (pulse) and the frequency-domain (phase/modulation) methods of time-resolved fluorescence spectroscopy. All possible wavelengths are available to excite fluorescent biological probes since synchrotron radiation consists of an intense continuum of energies from the IR to X-rays. The pulsed nature of synchrotron radiation permits time-correlated single photoncounting techniques to collect a histogram of the probability of the decay of the excited state. The harmonics of the pulsed exciting light also provide a wide range of modulation frequencies that permit the frequency-domain method to match the current resolution for time-domain measurements.

Original languageEnglish (US)
Pages (from-to)29-33
Number of pages5
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume909
DOIs
StatePublished - Jun 24 1988
Externally publishedYes
EventTime-Resolved Laser Spectroscopy in Biochemistry 1988 - Los Angeles, United States
Duration: Jan 11 1988Jan 17 1988

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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