Synchrotron radiation circular dichroism (SRCD) spectroscopy: An emerging method in structural biology for examining protein conformations and protein interactions

B. A. Wallace, Kunihiko Gekko, Søren Vrønning Hoffmann, Yi Hung Lin, John C. Sutherland, Ye Tao, Frank Wien, Robert W. Janes

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Circular dichroism (CD) spectroscopy is a well-established technique in structural biology. The use of synchrotron radiation as an intense light source for these measurements extends the applications possible using lab-based instruments. In recent years, there has been a major growth in synchrotron radiation circular dichroism (SRCD) beamlines worldwide, including ones at the NSLS, ISA, SRS, HiSOR, BSRF, NSRRC, SOLEIL, Diamond, TERAS, BESSYII, and ANKA synchrotrons. Through the coordinated efforts of beamline scientists and users at these sites, important proof-of-principle studies have been done enabling the method to be developed for novel and productive studies on biological systems. This paper describes the characteristics of SRCD beamlines and some of the new types of applications that have been undertaken using these beamlines.

Original languageEnglish (US)
Pages (from-to)177-178
Number of pages2
JournalNuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment
Volume649
Issue number1
DOIs
StatePublished - Sep 1 2011
Externally publishedYes

Fingerprint

Circular dichroism spectroscopy
Synchrotron radiation
biology
dichroism
Conformations
emerging
synchrotron radiation
Dichroism
proteins
Proteins
spectroscopy
interactions
Biological systems
Synchrotrons
Light sources
Diamonds
light sources
synchrotrons
diamonds

Keywords

  • Conformational changes
  • Macromolecular interactions
  • Protein secondary structure
  • Synchrotron radiation circular dichroism spectroscopy

ASJC Scopus subject areas

  • Nuclear and High Energy Physics
  • Instrumentation

Cite this

Synchrotron radiation circular dichroism (SRCD) spectroscopy : An emerging method in structural biology for examining protein conformations and protein interactions. / Wallace, B. A.; Gekko, Kunihiko; Vrønning Hoffmann, Søren; Lin, Yi Hung; Sutherland, John C.; Tao, Ye; Wien, Frank; Janes, Robert W.

In: Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, Vol. 649, No. 1, 01.09.2011, p. 177-178.

Research output: Contribution to journalArticle

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