Synergistic effect of high charge and energy particle radiation and chronological age on biomarkers of oxidative stress and tissue degeneration

A ground-based study using the vertebrate laboratory model organism Oryzias latipes

Xuan Zheng, Xinyan Zhang, Lingling Ding, Jeffrey R Lee, Paul Maurice Weinberger, William S. Dynan

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

High charge and energy (HZE) particles are a main hazard of the space radiation environment. Uncertainty regarding their health effects is a limiting factor in the design of human exploration-class space missions, that is, missions beyond low earth orbit. Previous work has shown that HZE exposure increases cancer risk and elicits other aging-like phenomena in animal models. Here, we investigate how a single exposure to HZE particle radiation, early in life, influences the subsequent agedependent evolution of oxidative stress and appearance of degenerative tissue changes. Embryos of the laboratory model organism, Oryzias latipes (Japanese medaka fish), were exposed to HZE particle radiation at doses overlapping the range of anticipated human exposure. A separate cohort was exposed to reference γradiation. Survival was monitored for 750 days, well beyond the median lifespan. The population was also sampled at intervals and liver tissue was subjected to histological and molecular analysis. HZE particle radiation dose and aging contributed synergistically to accumulation of lipid peroxidation products, which are a marker of chronic oxidative stress. This was mirrored by a decline in PPARGC1A mRNA, which encodes a transcriptional co-activator required for expression of oxidative stress defense genes and for mitochondrial maintenance. Consistent with chronic oxidative stress, mitochondria had an elongated and enlarged ultrastructure. Livers also had distinctive, cystic lesions. Depending on the endpoint, effects of γrays in the same dose range were either lesser or not detected. Results provide a quantitative and qualitative framework for understanding relative contributions of HZE particle radiation exposure and aging to chronic oxidative stress and tissue degeneration.

Original languageEnglish (US)
Article numbere111362
JournalPloS one
Volume9
Issue number11
DOIs
StatePublished - Nov 6 2014

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Cosmic Radiation
tissue degeneration
Oryzias
Oryzias latipes
Oxidative stress
Biomarkers
Vertebrates
biomarkers
Oxidative Stress
oxidative stress
vertebrates
Radiation
Tissue
organisms
energy
Aging of materials
Liver
Dosimetry
dosage
Space Flight

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Synergistic effect of high charge and energy particle radiation and chronological age on biomarkers of oxidative stress and tissue degeneration : A ground-based study using the vertebrate laboratory model organism Oryzias latipes. / Zheng, Xuan; Zhang, Xinyan; Ding, Lingling; Lee, Jeffrey R; Weinberger, Paul Maurice; Dynan, William S.

In: PloS one, Vol. 9, No. 11, e111362, 06.11.2014.

Research output: Contribution to journalArticle

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