Targeted deletion of Hsf1, 2, and 4 genes in mice

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Citations (Scopus)

Abstract

Heat-shock transcription factors (Hsfs) regulate transcription of heat-shock proteins as well as other genes whose promoters contain heat-shock elements. There are at least five Hsfs in mammalian cells, Hsf1, Hsf2, Hsf3, Hsf4, and Hsfy. To understand the physiological roles of Hsf1, Hsf2, and Hsf4 in vivo, we generated knockout mouse lines for these factors. In this chapter, we describe the design of the targeting vectors, the plasmids used, and the successful generation of mice lacking the individual genes. We also briefly describe what we have learned about the physiological functions of these genes in vivo.

Original languageEnglish (US)
Title of host publicationMolecular Chaperones
Subtitle of host publicationMethods and Protocols
EditorsStuart K. Calderwood, Thomas L. Prince
Pages1-20
Number of pages20
DOIs
StatePublished - Oct 25 2011

Publication series

NameMethods in Molecular Biology
Volume787
ISSN (Print)1064-3745

Fingerprint

Genes
Heat-Shock Proteins
Knockout Mice
Shock
Plasmids
Hot Temperature
heat shock transcription factor

Keywords

  • Hsf1
  • Hsf2
  • Hsf4
  • Hsf4-EGFP
  • Knockout mice
  • Targeting vector

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Jin, X., Eroglu, B., Moskofidis, D., & Mivechi, N. F. (2011). Targeted deletion of Hsf1, 2, and 4 genes in mice. In S. K. Calderwood, & T. L. Prince (Eds.), Molecular Chaperones: Methods and Protocols (pp. 1-20). (Methods in Molecular Biology; Vol. 787). https://doi.org/10.1007/978-1-61779-295-3_1

Targeted deletion of Hsf1, 2, and 4 genes in mice. / Jin, Xiongjie; Eroglu, Binnur; Moskofidis, Dimitrios; Mivechi, Nahid F.

Molecular Chaperones: Methods and Protocols. ed. / Stuart K. Calderwood; Thomas L. Prince. 2011. p. 1-20 (Methods in Molecular Biology; Vol. 787).

Research output: Chapter in Book/Report/Conference proceedingChapter

Jin, X, Eroglu, B, Moskofidis, D & Mivechi, NF 2011, Targeted deletion of Hsf1, 2, and 4 genes in mice. in SK Calderwood & TL Prince (eds), Molecular Chaperones: Methods and Protocols. Methods in Molecular Biology, vol. 787, pp. 1-20. https://doi.org/10.1007/978-1-61779-295-3_1
Jin X, Eroglu B, Moskofidis D, Mivechi NF. Targeted deletion of Hsf1, 2, and 4 genes in mice. In Calderwood SK, Prince TL, editors, Molecular Chaperones: Methods and Protocols. 2011. p. 1-20. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-295-3_1
Jin, Xiongjie ; Eroglu, Binnur ; Moskofidis, Dimitrios ; Mivechi, Nahid F. / Targeted deletion of Hsf1, 2, and 4 genes in mice. Molecular Chaperones: Methods and Protocols. editor / Stuart K. Calderwood ; Thomas L. Prince. 2011. pp. 1-20 (Methods in Molecular Biology).
@inbook{5a0dcd09084a40379de02ee961fb3277,
title = "Targeted deletion of Hsf1, 2, and 4 genes in mice",
abstract = "Heat-shock transcription factors (Hsfs) regulate transcription of heat-shock proteins as well as other genes whose promoters contain heat-shock elements. There are at least five Hsfs in mammalian cells, Hsf1, Hsf2, Hsf3, Hsf4, and Hsfy. To understand the physiological roles of Hsf1, Hsf2, and Hsf4 in vivo, we generated knockout mouse lines for these factors. In this chapter, we describe the design of the targeting vectors, the plasmids used, and the successful generation of mice lacking the individual genes. We also briefly describe what we have learned about the physiological functions of these genes in vivo.",
keywords = "Hsf1, Hsf2, Hsf4, Hsf4-EGFP, Knockout mice, Targeting vector",
author = "Xiongjie Jin and Binnur Eroglu and Dimitrios Moskofidis and Mivechi, {Nahid F}",
year = "2011",
month = "10",
day = "25",
doi = "10.1007/978-1-61779-295-3_1",
language = "English (US)",
isbn = "9781617792946",
series = "Methods in Molecular Biology",
pages = "1--20",
editor = "Calderwood, {Stuart K.} and Prince, {Thomas L.}",
booktitle = "Molecular Chaperones",

}

TY - CHAP

T1 - Targeted deletion of Hsf1, 2, and 4 genes in mice

AU - Jin, Xiongjie

AU - Eroglu, Binnur

AU - Moskofidis, Dimitrios

AU - Mivechi, Nahid F

PY - 2011/10/25

Y1 - 2011/10/25

N2 - Heat-shock transcription factors (Hsfs) regulate transcription of heat-shock proteins as well as other genes whose promoters contain heat-shock elements. There are at least five Hsfs in mammalian cells, Hsf1, Hsf2, Hsf3, Hsf4, and Hsfy. To understand the physiological roles of Hsf1, Hsf2, and Hsf4 in vivo, we generated knockout mouse lines for these factors. In this chapter, we describe the design of the targeting vectors, the plasmids used, and the successful generation of mice lacking the individual genes. We also briefly describe what we have learned about the physiological functions of these genes in vivo.

AB - Heat-shock transcription factors (Hsfs) regulate transcription of heat-shock proteins as well as other genes whose promoters contain heat-shock elements. There are at least five Hsfs in mammalian cells, Hsf1, Hsf2, Hsf3, Hsf4, and Hsfy. To understand the physiological roles of Hsf1, Hsf2, and Hsf4 in vivo, we generated knockout mouse lines for these factors. In this chapter, we describe the design of the targeting vectors, the plasmids used, and the successful generation of mice lacking the individual genes. We also briefly describe what we have learned about the physiological functions of these genes in vivo.

KW - Hsf1

KW - Hsf2

KW - Hsf4

KW - Hsf4-EGFP

KW - Knockout mice

KW - Targeting vector

UR - http://www.scopus.com/inward/record.url?scp=80054780964&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80054780964&partnerID=8YFLogxK

U2 - 10.1007/978-1-61779-295-3_1

DO - 10.1007/978-1-61779-295-3_1

M3 - Chapter

C2 - 21898223

AN - SCOPUS:80054780964

SN - 9781617792946

T3 - Methods in Molecular Biology

SP - 1

EP - 20

BT - Molecular Chaperones

A2 - Calderwood, Stuart K.

A2 - Prince, Thomas L.

ER -