Targeted deletion of Hsf1, 2, and 4 genes in mice

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Scopus citations

Abstract

Heat-shock transcription factors (Hsfs) regulate transcription of heat-shock proteins as well as other genes whose promoters contain heat-shock elements. There are at least five Hsfs in mammalian cells, Hsf1, Hsf2, Hsf3, Hsf4, and Hsfy. To understand the physiological roles of Hsf1, Hsf2, and Hsf4 in vivo, we generated knockout mouse lines for these factors. In this chapter, we describe the design of the targeting vectors, the plasmids used, and the successful generation of mice lacking the individual genes. We also briefly describe what we have learned about the physiological functions of these genes in vivo.

Original languageEnglish (US)
Title of host publicationMolecular Chaperones
Subtitle of host publicationMethods and Protocols
EditorsStuart K. Calderwood, Thomas L. Prince
Pages1-20
Number of pages20
DOIs
StatePublished - Oct 25 2011

Publication series

NameMethods in Molecular Biology
Volume787
ISSN (Print)1064-3745

Keywords

  • Hsf1
  • Hsf2
  • Hsf4
  • Hsf4-EGFP
  • Knockout mice
  • Targeting vector

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Jin, X., Eroglu, B., Moskophidis, D., & Mivechi, N. F. (2011). Targeted deletion of Hsf1, 2, and 4 genes in mice. In S. K. Calderwood, & T. L. Prince (Eds.), Molecular Chaperones: Methods and Protocols (pp. 1-20). (Methods in Molecular Biology; Vol. 787). https://doi.org/10.1007/978-1-61779-295-3_1