Abstract
The plasmid pNDTM2 was constructed to contain the pR-pL promoter and the phbC, A, and B genes which code for PHB synthase, β-ketothiolase, and acetoacetyl-CoA reductase, respectively. This plasmid was transformed into Escherichia coli XL1-Blue. The effect of several thermal induction patterns on the production of poly-3-hydroxybutyric acid (PHB) was investigated based on fermentor experiments. Based on the experimental results with different induction patterns, it was found that the temperature should be controlled at 34°C during the initial 10 h of cultivation to promote cell growth, and then it should be increased to 40°C for induction. Then the temperature should be lowered to 37°C after 5 h to relieve the effect of the heat shock.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 21-26 |
| Number of pages | 6 |
| Journal | Journal of Bioscience and Bioengineering |
| Volume | 91 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 2001 |
| Externally published | Yes |
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Keywords
- PHB
- Recombinant Escherichia coli
- Temperature pattern
- p-p promoter
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
Cite this
Temperature-induced expression of phb genes in Escherichia coli and the effect of temperature patterns on the production of poly-3-hydroxybutyrate. / Shi, Huidong; Kyuwa, Kaoru; Takasu, Mitsuhiro; Shimizu, Kazuyuki.
In: Journal of Bioscience and Bioengineering, Vol. 91, No. 1, 01.01.2001, p. 21-26.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Temperature-induced expression of phb genes in Escherichia coli and the effect of temperature patterns on the production of poly-3-hydroxybutyrate
AU - Shi, Huidong
AU - Kyuwa, Kaoru
AU - Takasu, Mitsuhiro
AU - Shimizu, Kazuyuki
PY - 2001/1/1
Y1 - 2001/1/1
N2 - The plasmid pNDTM2 was constructed to contain the pR-pL promoter and the phbC, A, and B genes which code for PHB synthase, β-ketothiolase, and acetoacetyl-CoA reductase, respectively. This plasmid was transformed into Escherichia coli XL1-Blue. The effect of several thermal induction patterns on the production of poly-3-hydroxybutyric acid (PHB) was investigated based on fermentor experiments. Based on the experimental results with different induction patterns, it was found that the temperature should be controlled at 34°C during the initial 10 h of cultivation to promote cell growth, and then it should be increased to 40°C for induction. Then the temperature should be lowered to 37°C after 5 h to relieve the effect of the heat shock.
AB - The plasmid pNDTM2 was constructed to contain the pR-pL promoter and the phbC, A, and B genes which code for PHB synthase, β-ketothiolase, and acetoacetyl-CoA reductase, respectively. This plasmid was transformed into Escherichia coli XL1-Blue. The effect of several thermal induction patterns on the production of poly-3-hydroxybutyric acid (PHB) was investigated based on fermentor experiments. Based on the experimental results with different induction patterns, it was found that the temperature should be controlled at 34°C during the initial 10 h of cultivation to promote cell growth, and then it should be increased to 40°C for induction. Then the temperature should be lowered to 37°C after 5 h to relieve the effect of the heat shock.
KW - PHB
KW - Recombinant Escherichia coli
KW - Temperature pattern
KW - p-p promoter
UR - http://www.scopus.com/inward/record.url?scp=0035113267&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035113267&partnerID=8YFLogxK
U2 - 10.1016/S1389-1723(01)80105-5
DO - 10.1016/S1389-1723(01)80105-5
M3 - Article
C2 - 16232940
AN - SCOPUS:0035113267
VL - 91
SP - 21
EP - 26
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 1
ER -