Abstract
The plasmid pNDTM2 was constructed to contain the pR-pL promoter and the phbC, A, and B genes which code for PHB synthase, β-ketothiolase, and acetoacetyl-CoA reductase, respectively. This plasmid was transformed into Escherichia coli XL1-Blue. The effect of several thermal induction patterns on the production of poly-3-hydroxybutyric acid (PHB) was investigated based on fermentor experiments. Based on the experimental results with different induction patterns, it was found that the temperature should be controlled at 34°C during the initial 10 h of cultivation to promote cell growth, and then it should be increased to 40°C for induction. Then the temperature should be lowered to 37°C after 5 h to relieve the effect of the heat shock.
Original language | English (US) |
---|---|
Pages (from-to) | 21-26 |
Number of pages | 6 |
Journal | Journal of Bioscience and Bioengineering |
Volume | 91 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1 2001 |
Externally published | Yes |
Keywords
- PHB
- Recombinant Escherichia coli
- Temperature pattern
- p-p promoter
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology