Temperature-induced expression of phb genes in Escherichia coli and the effect of temperature patterns on the production of poly-3-hydroxybutyrate

Huidong Shi, Kaoru Kyuwa, Mitsuhiro Takasu, Kazuyuki Shimizu

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The plasmid pNDTM2 was constructed to contain the pR-pL promoter and the phbC, A, and B genes which code for PHB synthase, β-ketothiolase, and acetoacetyl-CoA reductase, respectively. This plasmid was transformed into Escherichia coli XL1-Blue. The effect of several thermal induction patterns on the production of poly-3-hydroxybutyric acid (PHB) was investigated based on fermentor experiments. Based on the experimental results with different induction patterns, it was found that the temperature should be controlled at 34°C during the initial 10 h of cultivation to promote cell growth, and then it should be increased to 40°C for induction. Then the temperature should be lowered to 37°C after 5 h to relieve the effect of the heat shock.

Original languageEnglish (US)
Pages (from-to)21-26
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume91
Issue number1
DOIs
StatePublished - Jan 1 2001
Externally publishedYes

Keywords

  • PHB
  • Recombinant Escherichia coli
  • Temperature pattern
  • p-p promoter

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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