Abstract
The plasmid pNDTM2 was constructed to contain the pR-pL promoter and the phbC, A, and B genes which code for PHB synthase, β-ketothiolase, and acetoacetyl-CoA reductase, respectively. This plasmid was transformed into Escherichia coli XL1-Blue. The effect of several thermal induction patterns on the production of poly-3-hydroxybutyric acid (PHB) was investigated based on fermentor experiments. Based on the experimental results with different induction patterns, it was found that the temperature should be controlled at 34°C during the initial 10 h of cultivation to promote cell growth, and then it should be increased to 40°C for induction. Then the temperature should be lowered to 37°C after 5 h to relieve the effect of the heat shock.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 21-26 |
| Number of pages | 6 |
| Journal | Journal of Bioscience and Bioengineering |
| Volume | 91 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 2001 |
| Externally published | Yes |
Keywords
- PHB
- Recombinant Escherichia coli
- Temperature pattern
- p-p promoter
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
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Cite this
Shi, H., Kyuwa, K., Takasu, M., & Shimizu, K. (2001). Temperature-induced expression of phb genes in Escherichia coli and the effect of temperature patterns on the production of poly-3-hydroxybutyrate. Journal of Bioscience and Bioengineering, 91(1), 21-26. https://doi.org/10.1016/S1389-1723(01)80105-5