Temperature-induced expression of phb genes in Escherichia coli and the effect of temperature patterns on the production of poly-3-hydroxybutyrate

Huidong Shi; Kaoru Kyuwa; Mitsuhiro Takasu; Kazuyuki Shimizu

doi:10.1016/S1389-1723(01)80105-5

Temperature-induced expression of phb genes in Escherichia coli and the effect of temperature patterns on the production of poly-3-hydroxybutyrate

Huidong Shi, Kaoru Kyuwa, Mitsuhiro Takasu, Kazuyuki Shimizu

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The plasmid pNDTM2 was constructed to contain the p_R-p_L promoter and the phbC, A, and B genes which code for PHB synthase, β-ketothiolase, and acetoacetyl-CoA reductase, respectively. This plasmid was transformed into Escherichia coli XL1-Blue. The effect of several thermal induction patterns on the production of poly-3-hydroxybutyric acid (PHB) was investigated based on fermentor experiments. Based on the experimental results with different induction patterns, it was found that the temperature should be controlled at 34°C during the initial 10 h of cultivation to promote cell growth, and then it should be increased to 40°C for induction. Then the temperature should be lowered to 37°C after 5 h to relieve the effect of the heat shock.

Original language	English (US)
Pages (from-to)	21-26
Number of pages	6
Journal	Journal of Bioscience and Bioengineering
Volume	91
Issue number	1
DOIs	https://doi.org/10.1016/S1389-1723(01)80105-5
State	Published - Jan 1 2001
Externally published	Yes

Keywords

PHB
Recombinant Escherichia coli
Temperature pattern
p-p promoter

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

Access to Document

10.1016/S1389-1723(01)80105-5

Fingerprint Dive into the research topics of 'Temperature-induced expression of phb genes in Escherichia coli and the effect of temperature patterns on the production of poly-3-hydroxybutyrate'. Together they form a unique fingerprint.

Cite this

@article{fbbc91d898ab44f290838d64df4827a8,

title = "Temperature-induced expression of phb genes in Escherichia coli and the effect of temperature patterns on the production of poly-3-hydroxybutyrate",

abstract = "The plasmid pNDTM2 was constructed to contain the pR-pL promoter and the phbC, A, and B genes which code for PHB synthase, β-ketothiolase, and acetoacetyl-CoA reductase, respectively. This plasmid was transformed into Escherichia coli XL1-Blue. The effect of several thermal induction patterns on the production of poly-3-hydroxybutyric acid (PHB) was investigated based on fermentor experiments. Based on the experimental results with different induction patterns, it was found that the temperature should be controlled at 34°C during the initial 10 h of cultivation to promote cell growth, and then it should be increased to 40°C for induction. Then the temperature should be lowered to 37°C after 5 h to relieve the effect of the heat shock.",

keywords = "PHB, Recombinant Escherichia coli, Temperature pattern, p-p promoter",

author = "Huidong Shi and Kaoru Kyuwa and Mitsuhiro Takasu and Kazuyuki Shimizu",

year = "2001",

month = jan,

day = "1",

doi = "10.1016/S1389-1723(01)80105-5",

language = "English (US)",

volume = "91",

pages = "21--26",

journal = "Journal of Bioscience and Bioengineering",

issn = "1389-1723",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Temperature-induced expression of phb genes in Escherichia coli and the effect of temperature patterns on the production of poly-3-hydroxybutyrate

AU - Shi, Huidong

AU - Kyuwa, Kaoru

AU - Takasu, Mitsuhiro

AU - Shimizu, Kazuyuki

PY - 2001/1/1

Y1 - 2001/1/1

N2 - The plasmid pNDTM2 was constructed to contain the pR-pL promoter and the phbC, A, and B genes which code for PHB synthase, β-ketothiolase, and acetoacetyl-CoA reductase, respectively. This plasmid was transformed into Escherichia coli XL1-Blue. The effect of several thermal induction patterns on the production of poly-3-hydroxybutyric acid (PHB) was investigated based on fermentor experiments. Based on the experimental results with different induction patterns, it was found that the temperature should be controlled at 34°C during the initial 10 h of cultivation to promote cell growth, and then it should be increased to 40°C for induction. Then the temperature should be lowered to 37°C after 5 h to relieve the effect of the heat shock.

AB - The plasmid pNDTM2 was constructed to contain the pR-pL promoter and the phbC, A, and B genes which code for PHB synthase, β-ketothiolase, and acetoacetyl-CoA reductase, respectively. This plasmid was transformed into Escherichia coli XL1-Blue. The effect of several thermal induction patterns on the production of poly-3-hydroxybutyric acid (PHB) was investigated based on fermentor experiments. Based on the experimental results with different induction patterns, it was found that the temperature should be controlled at 34°C during the initial 10 h of cultivation to promote cell growth, and then it should be increased to 40°C for induction. Then the temperature should be lowered to 37°C after 5 h to relieve the effect of the heat shock.

KW - PHB

KW - Recombinant Escherichia coli

KW - Temperature pattern

KW - p-p promoter

UR - http://www.scopus.com/inward/record.url?scp=0035113267&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035113267&partnerID=8YFLogxK

U2 - 10.1016/S1389-1723(01)80105-5

DO - 10.1016/S1389-1723(01)80105-5

M3 - Article

C2 - 16232940

AN - SCOPUS:0035113267

VL - 91

SP - 21

EP - 26

JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

SN - 1389-1723

IS - 1

ER -

Temperature-induced expression of phb genes in Escherichia coli and the effect of temperature patterns on the production of poly-3-hydroxybutyrate

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Cite this