The angiotensin II AT1 receptor is tyrosine and serine phosphorylated and can serve as a substrate for the src family of tyrosine kinases

William G. Paxton, Mario B. Marrero, Janet D. Klein, Patrick Delafontaine, Bradford C. Berk, Kenneth E. Bernstein

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Angiotensin II AT1 receptor signal transduction has recently been shown to function through the phospholipase C isozyme, PLC-γ. Since PLC-γ is known to interact with phosphotyrosine containing proteins through SH2 domains, we examined the phosphorylation state of the AT1 receptor. Immunoprecipitation of the [32P] labeled AT1 receptor from rat aortic smooth muscle cells followed by alkali hydrolysis demonstrated the presence of tyrosine phosphorylation. Phosphoamino acid analysis of the excised bands demonstrated the presence of phosphoserine and phosphotyrosine residues. A fusion protein comprising the intracellular tail of the AT1 receptor was used to screen for candidate kinases, and the src kinase family displayed high activity. In summary, this study shows that the AT1 receptor is serine and tyrosine phosphorylated in vivo and suggests that a soluble kinase related to the src family may be responsible for the tyrosine phosphorylation.

Original languageEnglish (US)
Pages (from-to)260-267
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume200
Issue number1
DOIs
StatePublished - Apr 15 1994

Fingerprint

Angiotensin Type 1 Receptor
Phosphorylation
Angiotensin Receptors
src-Family Kinases
Angiotensin II
Serine
Tyrosine
Phosphotyrosine
Programmable logic controllers
Phosphotransferases
Substrates
Phosphoamino Acids
Phosphoserine
Signal transduction
src Homology Domains
Alkalies
Type C Phospholipases
Immunoprecipitation
Isoenzymes
Smooth Muscle Myocytes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

The angiotensin II AT1 receptor is tyrosine and serine phosphorylated and can serve as a substrate for the src family of tyrosine kinases. / Paxton, William G.; Marrero, Mario B.; Klein, Janet D.; Delafontaine, Patrick; Berk, Bradford C.; Bernstein, Kenneth E.

In: Biochemical and Biophysical Research Communications, Vol. 200, No. 1, 15.04.1994, p. 260-267.

Research output: Contribution to journalArticle

Paxton, William G. ; Marrero, Mario B. ; Klein, Janet D. ; Delafontaine, Patrick ; Berk, Bradford C. ; Bernstein, Kenneth E. / The angiotensin II AT1 receptor is tyrosine and serine phosphorylated and can serve as a substrate for the src family of tyrosine kinases. In: Biochemical and Biophysical Research Communications. 1994 ; Vol. 200, No. 1. pp. 260-267.
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