The autoimmune regulator directly controls the expression of genes critical for thymic epithelial function

Qing Guo Ruan, Kenneth Tung, Daniel Elsenman, Yulius Setiady, Sarah Eckenrode, Bing Yi, Sharad B Purohit, Wei Peng Zheng, Yan Zhang, Leena Peltonen, Jin-Xiong She

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

The autoimmune regulator (Aire) gene plays an essential role in negative selection of T cells and deletion of autoreactive T cells in the thymus. The defect in thymic selection in Aire-/- mice was attributed to the repressed expression of tissue-specific Ags in the thymic epithelial cells and defective Ag presentation; however, the molecular mechanism underlying these functions has been elusive. Using the chromatin immunoprecipitation technique, we demonstrate here that Aire binds in vivo to specific DNA sequence motifs and directly regulates thymic expression of genes important for thymic functions including expression of autoantigens, cytokines, transcription factors, and posttranslational modifiers. These results unambiguously established Aire as a key transcriptional regulator of the immune system.

Original languageEnglish (US)
Pages (from-to)7173-7180
Number of pages8
JournalJournal of Immunology
Volume178
Issue number11
DOIs
StatePublished - Jun 1 2007

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Ruan, Q. G., Tung, K., Elsenman, D., Setiady, Y., Eckenrode, S., Yi, B., Purohit, S. B., Zheng, W. P., Zhang, Y., Peltonen, L., & She, J-X. (2007). The autoimmune regulator directly controls the expression of genes critical for thymic epithelial function. Journal of Immunology, 178(11), 7173-7180. https://doi.org/10.4049/jimmunol.178.11.7173