The construction of cosmid libraries which can be used to transform eukaryotic cells

F. G. Grosveld; T. Lund; E. J. Murray; A. L. Mellor; H. H M Dahl; R. A. Flavell

doi:10.1093/nar/10.21.6715

The construction of cosmid libraries which can be used to transform eukaryotic cells

F. G. Grosveld, T. Lund, E. J. Murray, A. L. Mellor, H. H M Dahl, R. A. Flavell

Infectious Disease

Research output: Contribution to journal › Article › peer-review

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Abstract

Cosmid vectors have been developed which carry selective markers for growth in bacteria (β lactamase gene) and animal cells (the Herpes Simplex virus thymidine kinase gene, the transposon Tn-5 aminoglycosyl 3′ phosphotransrefase gene and the E. coli guanine phosphoribosyltransferase gene). The design of the cosmids allows the exchange of the eukaryotic markers in recombinant cosmids. Human and mouse cosmid libraries containing DNA inserts of about 40kb have been generated by an improved method. Several clones from the human β-globin locus were isolated. These cosmids transform mouse L cells at high efficiency in both circular and linear form. The newly introduced genes are expressed accurately in L cells.

Original language	English (US)
Pages (from-to)	6715-6732
Number of pages	18
Journal	Nucleic Acids Research
Volume	10
Issue number	21
DOIs	https://doi.org/10.1093/nar/10.21.6715
State	Published - Nov 11 1982

ASJC Scopus subject areas

Genetics

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title = "The construction of cosmid libraries which can be used to transform eukaryotic cells",

abstract = "Cosmid vectors have been developed which carry selective markers for growth in bacteria (β lactamase gene) and animal cells (the Herpes Simplex virus thymidine kinase gene, the transposon Tn-5 aminoglycosyl 3′ phosphotransrefase gene and the E. coli guanine phosphoribosyltransferase gene). The design of the cosmids allows the exchange of the eukaryotic markers in recombinant cosmids. Human and mouse cosmid libraries containing DNA inserts of about 40kb have been generated by an improved method. Several clones from the human β-globin locus were isolated. These cosmids transform mouse L cells at high efficiency in both circular and linear form. The newly introduced genes are expressed accurately in L cells.",

author = "Grosveld, {F. G.} and T. Lund and Murray, {E. J.} and Mellor, {A. L.} and Dahl, {H. H M} and Flavell, {R. A.}",

note = "Funding Information: ACKNOWLEDGEMENTS T.L. was a grateful recipient of an EMBO long term fellowship. We thank Drs. C. Weissmann, P. Berg, D. Ish Horowicz, A. Garapin, F. Bolivar and J. Collins for their generous gifts of the different plasmids and C. 0'Carroll for preparing the manuscript. This work was supported by the British Medical Research Council.",

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AU - Mellor, A. L.

AU - Dahl, H. H M

AU - Flavell, R. A.

N1 - Funding Information: ACKNOWLEDGEMENTS T.L. was a grateful recipient of an EMBO long term fellowship. We thank Drs. C. Weissmann, P. Berg, D. Ish Horowicz, A. Garapin, F. Bolivar and J. Collins for their generous gifts of the different plasmids and C. 0'Carroll for preparing the manuscript. This work was supported by the British Medical Research Council.

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The construction of cosmid libraries which can be used to transform eukaryotic cells

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