The construction of cosmid libraries which can be used to transform eukaryotic cells

F. G. Grosveld; T. Lund; E. J. Murray; A. L. Mellor; H. H M Dahl; R. A. Flavell

doi:10.1093/nar/10.21.6715

The construction of cosmid libraries which can be used to transform eukaryotic cells

F. G. Grosveld, T. Lund, E. J. Murray, A. L. Mellor, H. H M Dahl, R. A. Flavell

Infectious Disease

Research output: Contribution to journal › Article › peer-review

186 Scopus citations

Abstract

Cosmid vectors have been developed which carry selective markers for growth in bacteria (β lactamase gene) and animal cells (the Herpes Simplex virus thymidine kinase gene, the transposon Tn-5 aminoglycosyl 3′ phosphotransrefase gene and the E. coli guanine phosphoribosyltransferase gene). The design of the cosmids allows the exchange of the eukaryotic markers in recombinant cosmids. Human and mouse cosmid libraries containing DNA inserts of about 40kb have been generated by an improved method. Several clones from the human β-globin locus were isolated. These cosmids transform mouse L cells at high efficiency in both circular and linear form. The newly introduced genes are expressed accurately in L cells.

Original language	English (US)
Pages (from-to)	6715-6732
Number of pages	18
Journal	Nucleic Acids Research
Volume	10
Issue number	21
DOIs	https://doi.org/10.1093/nar/10.21.6715
State	Published - Nov 11 1982

ASJC Scopus subject areas

Genetics

Access to Document

10.1093/nar/10.21.6715

Fingerprint Dive into the research topics of 'The construction of cosmid libraries which can be used to transform eukaryotic cells'. Together they form a unique fingerprint.

Cite this

@article{cb75c46bf2a04a34b9db3cc8f7a9cc08,

title = "The construction of cosmid libraries which can be used to transform eukaryotic cells",

abstract = "Cosmid vectors have been developed which carry selective markers for growth in bacteria (β lactamase gene) and animal cells (the Herpes Simplex virus thymidine kinase gene, the transposon Tn-5 aminoglycosyl 3′ phosphotransrefase gene and the E. coli guanine phosphoribosyltransferase gene). The design of the cosmids allows the exchange of the eukaryotic markers in recombinant cosmids. Human and mouse cosmid libraries containing DNA inserts of about 40kb have been generated by an improved method. Several clones from the human β-globin locus were isolated. These cosmids transform mouse L cells at high efficiency in both circular and linear form. The newly introduced genes are expressed accurately in L cells.",

author = "Grosveld, {F. G.} and T. Lund and Murray, {E. J.} and Mellor, {A. L.} and Dahl, {H. H M} and Flavell, {R. A.}",

note = "Funding Information: ACKNOWLEDGEMENTS T.L. was a grateful recipient of an EMBO long term fellowship. We thank Drs. C. Weissmann, P. Berg, D. Ish Horowicz, A. Garapin, F. Bolivar and J. Collins for their generous gifts of the different plasmids and C. 0'Carroll for preparing the manuscript. This work was supported by the British Medical Research Council.",

year = "1982",

month = nov,

day = "11",

doi = "10.1093/nar/10.21.6715",

language = "English (US)",

volume = "10",

pages = "6715--6732",

journal = "Nucleic Acids Research",

issn = "0305-1048",

publisher = "Oxford University Press",

number = "21",

}

TY - JOUR

T1 - The construction of cosmid libraries which can be used to transform eukaryotic cells

AU - Grosveld, F. G.

AU - Lund, T.

AU - Murray, E. J.

AU - Mellor, A. L.

AU - Dahl, H. H M

AU - Flavell, R. A.

N1 - Funding Information: ACKNOWLEDGEMENTS T.L. was a grateful recipient of an EMBO long term fellowship. We thank Drs. C. Weissmann, P. Berg, D. Ish Horowicz, A. Garapin, F. Bolivar and J. Collins for their generous gifts of the different plasmids and C. 0'Carroll for preparing the manuscript. This work was supported by the British Medical Research Council.

PY - 1982/11/11

Y1 - 1982/11/11

N2 - Cosmid vectors have been developed which carry selective markers for growth in bacteria (β lactamase gene) and animal cells (the Herpes Simplex virus thymidine kinase gene, the transposon Tn-5 aminoglycosyl 3′ phosphotransrefase gene and the E. coli guanine phosphoribosyltransferase gene). The design of the cosmids allows the exchange of the eukaryotic markers in recombinant cosmids. Human and mouse cosmid libraries containing DNA inserts of about 40kb have been generated by an improved method. Several clones from the human β-globin locus were isolated. These cosmids transform mouse L cells at high efficiency in both circular and linear form. The newly introduced genes are expressed accurately in L cells.

AB - Cosmid vectors have been developed which carry selective markers for growth in bacteria (β lactamase gene) and animal cells (the Herpes Simplex virus thymidine kinase gene, the transposon Tn-5 aminoglycosyl 3′ phosphotransrefase gene and the E. coli guanine phosphoribosyltransferase gene). The design of the cosmids allows the exchange of the eukaryotic markers in recombinant cosmids. Human and mouse cosmid libraries containing DNA inserts of about 40kb have been generated by an improved method. Several clones from the human β-globin locus were isolated. These cosmids transform mouse L cells at high efficiency in both circular and linear form. The newly introduced genes are expressed accurately in L cells.

UR - http://www.scopus.com/inward/record.url?scp=0020480669&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020480669&partnerID=8YFLogxK

U2 - 10.1093/nar/10.21.6715

DO - 10.1093/nar/10.21.6715

M3 - Article

C2 - 6294612

AN - SCOPUS:0020480669

VL - 10

SP - 6715

EP - 6732

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 21

ER -