The construction of cosmid libraries which can be used to transform eukaryotic cells

F. G. Grosveld, T. Lund, E. J. Murray, A. L. Mellor, H. H M Dahl, R. A. Flavell

Research output: Contribution to journalArticle

186 Citations (Scopus)

Abstract

Cosmid vectors have been developed which carry selective markers for growth in bacteria (β lactamase gene) and animal cells (the Herpes Simplex virus thymidine kinase gene, the transposon Tn-5 aminoglycosyl 3′ phosphotransrefase gene and the E. coli guanine phosphoribosyltransferase gene). The design of the cosmids allows the exchange of the eukaryotic markers in recombinant cosmids. Human and mouse cosmid libraries containing DNA inserts of about 40kb have been generated by an improved method. Several clones from the human β-globin locus were isolated. These cosmids transform mouse L cells at high efficiency in both circular and linear form. The newly introduced genes are expressed accurately in L cells.

Original languageEnglish (US)
Pages (from-to)6715-6732
Number of pages18
JournalNucleic Acids Research
Volume10
Issue number21
DOIs
StatePublished - Nov 11 1982

Fingerprint

Cosmids
Eukaryotic Cells
Genes
Hypoxanthine Phosphoribosyltransferase
Globins
Thymidine Kinase
Simplexvirus
Gene Library
Clone Cells
Escherichia coli
Bacteria
Growth

ASJC Scopus subject areas

  • Genetics

Cite this

Grosveld, F. G., Lund, T., Murray, E. J., Mellor, A. L., Dahl, H. H. M., & Flavell, R. A. (1982). The construction of cosmid libraries which can be used to transform eukaryotic cells. Nucleic Acids Research, 10(21), 6715-6732. https://doi.org/10.1093/nar/10.21.6715

The construction of cosmid libraries which can be used to transform eukaryotic cells. / Grosveld, F. G.; Lund, T.; Murray, E. J.; Mellor, A. L.; Dahl, H. H M; Flavell, R. A.

In: Nucleic Acids Research, Vol. 10, No. 21, 11.11.1982, p. 6715-6732.

Research output: Contribution to journalArticle

Grosveld, FG, Lund, T, Murray, EJ, Mellor, AL, Dahl, HHM & Flavell, RA 1982, 'The construction of cosmid libraries which can be used to transform eukaryotic cells', Nucleic Acids Research, vol. 10, no. 21, pp. 6715-6732. https://doi.org/10.1093/nar/10.21.6715
Grosveld FG, Lund T, Murray EJ, Mellor AL, Dahl HHM, Flavell RA. The construction of cosmid libraries which can be used to transform eukaryotic cells. Nucleic Acids Research. 1982 Nov 11;10(21):6715-6732. https://doi.org/10.1093/nar/10.21.6715
Grosveld, F. G. ; Lund, T. ; Murray, E. J. ; Mellor, A. L. ; Dahl, H. H M ; Flavell, R. A. / The construction of cosmid libraries which can be used to transform eukaryotic cells. In: Nucleic Acids Research. 1982 ; Vol. 10, No. 21. pp. 6715-6732.
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