Abstract
Cosmid vectors have been developed which carry selective markers for growth in bacteria (β lactamase gene) and animal cells (the Herpes Simplex virus thymidine kinase gene, the transposon Tn-5 aminoglycosyl 3′ phosphotransrefase gene and the E. coli guanine phosphoribosyltransferase gene). The design of the cosmids allows the exchange of the eukaryotic markers in recombinant cosmids. Human and mouse cosmid libraries containing DNA inserts of about 40kb have been generated by an improved method. Several clones from the human β-globin locus were isolated. These cosmids transform mouse L cells at high efficiency in both circular and linear form. The newly introduced genes are expressed accurately in L cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 6715-6732 |
| Number of pages | 18 |
| Journal | Nucleic Acids Research |
| Volume | 10 |
| Issue number | 21 |
| DOIs | |
| State | Published - Nov 11 1982 |
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ASJC Scopus subject areas
- Genetics
Cite this
The construction of cosmid libraries which can be used to transform eukaryotic cells. / Grosveld, F. G.; Lund, T.; Murray, E. J.; Mellor, A. L.; Dahl, H. H M; Flavell, R. A.
In: Nucleic Acids Research, Vol. 10, No. 21, 11.11.1982, p. 6715-6732.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The construction of cosmid libraries which can be used to transform eukaryotic cells
AU - Grosveld, F. G.
AU - Lund, T.
AU - Murray, E. J.
AU - Mellor, A. L.
AU - Dahl, H. H M
AU - Flavell, R. A.
PY - 1982/11/11
Y1 - 1982/11/11
N2 - Cosmid vectors have been developed which carry selective markers for growth in bacteria (β lactamase gene) and animal cells (the Herpes Simplex virus thymidine kinase gene, the transposon Tn-5 aminoglycosyl 3′ phosphotransrefase gene and the E. coli guanine phosphoribosyltransferase gene). The design of the cosmids allows the exchange of the eukaryotic markers in recombinant cosmids. Human and mouse cosmid libraries containing DNA inserts of about 40kb have been generated by an improved method. Several clones from the human β-globin locus were isolated. These cosmids transform mouse L cells at high efficiency in both circular and linear form. The newly introduced genes are expressed accurately in L cells.
AB - Cosmid vectors have been developed which carry selective markers for growth in bacteria (β lactamase gene) and animal cells (the Herpes Simplex virus thymidine kinase gene, the transposon Tn-5 aminoglycosyl 3′ phosphotransrefase gene and the E. coli guanine phosphoribosyltransferase gene). The design of the cosmids allows the exchange of the eukaryotic markers in recombinant cosmids. Human and mouse cosmid libraries containing DNA inserts of about 40kb have been generated by an improved method. Several clones from the human β-globin locus were isolated. These cosmids transform mouse L cells at high efficiency in both circular and linear form. The newly introduced genes are expressed accurately in L cells.
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UR - http://www.scopus.com/inward/citedby.url?scp=0020480669&partnerID=8YFLogxK
U2 - 10.1093/nar/10.21.6715
DO - 10.1093/nar/10.21.6715
M3 - Article
VL - 10
SP - 6715
EP - 6732
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 21
ER -