The crucial role of vitamin C and its transporter (SVCT2) in bone marrow stromal cell autophagy and apoptosis

Rajnikumar Sangani, Sudharsan Periyasamy Thandavan, Rajneesh Pathania, Saif Ahmad, Ammar Kutiyanawalla, Ravindra Bharat Kolhe, Maryka H. Bhattacharyya, Norman Chutkan, David M Hunter, William D Hill, Mark W Hamrick, Carlos M Isales, Sadanand T Fulzele

Research output: Contribution to journalArticle

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Abstract

Vitamin C is an antioxidant that plays a vital role in various biological processes including bone formation. Previously, we reported that vitamin C is transported into bone marrow stromal cells (BMSCs) through the sodium dependent Vitamin C Transporter 2 (SVCT2) and this transporter plays an important role in osteogenic differentiation. Furthermore, this transporter is regulated by oxidative stress. To date, however, the exact role of vitamin C and its transporter (SVCT2) in ROS regulated autophagy and apoptosis in BMSCs is poorly understood. In the present study, we observed that oxidative stress decreased survival of BMSCs in a dose-dependent manner and induced growth arrest in the G1 phase of the cell cycle. These effects were accompanied by the induction of autophagy, confirmed by P62 and LC3B protein level and punctate GFP-LC3B distribution. The supplementation of vitamin C significantly rescued the BMSCs from oxidative stress by regulating autophagy. Knockdown of the SVCT2 transporter in BMSCs synergistically decreased cell survival even under low oxidative stress conditions. Also, supplementing vitamin C failed to rescue cells from stress. Our results reveal that the SVCT2 transporter plays a vital role in the mechanism of BMSC survival under stress conditions. Altogether, this study has given new insight into the role of the SVCT2 transporter in oxidative stress related autophagy and apoptosis in BMSCs.

Original languageEnglish (US)
Pages (from-to)312-321
Number of pages10
JournalStem Cell Research
Volume15
Issue number2
DOIs
StatePublished - Sep 1 2015

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Sodium-Coupled Vitamin C Transporters
Autophagy
Mesenchymal Stromal Cells
Ascorbic Acid
Apoptosis
Oxidative Stress
Cell Survival
Biological Phenomena
G1 Phase
Osteogenesis
Cell Cycle
Antioxidants

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

The crucial role of vitamin C and its transporter (SVCT2) in bone marrow stromal cell autophagy and apoptosis. / Sangani, Rajnikumar; Periyasamy Thandavan, Sudharsan; Pathania, Rajneesh; Ahmad, Saif; Kutiyanawalla, Ammar; Kolhe, Ravindra Bharat; Bhattacharyya, Maryka H.; Chutkan, Norman; Hunter, David M; Hill, William D; Hamrick, Mark W; Isales, Carlos M; Fulzele, Sadanand T.

In: Stem Cell Research, Vol. 15, No. 2, 01.09.2015, p. 312-321.

Research output: Contribution to journalArticle

Sangani, R, Periyasamy Thandavan, S, Pathania, R, Ahmad, S, Kutiyanawalla, A, Kolhe, RB, Bhattacharyya, MH, Chutkan, N, Hunter, DM, Hill, WD, Hamrick, MW, Isales, CM & Fulzele, ST 2015, 'The crucial role of vitamin C and its transporter (SVCT2) in bone marrow stromal cell autophagy and apoptosis', Stem Cell Research, vol. 15, no. 2, pp. 312-321. https://doi.org/10.1016/j.scr.2015.06.002
Sangani, Rajnikumar ; Periyasamy Thandavan, Sudharsan ; Pathania, Rajneesh ; Ahmad, Saif ; Kutiyanawalla, Ammar ; Kolhe, Ravindra Bharat ; Bhattacharyya, Maryka H. ; Chutkan, Norman ; Hunter, David M ; Hill, William D ; Hamrick, Mark W ; Isales, Carlos M ; Fulzele, Sadanand T. / The crucial role of vitamin C and its transporter (SVCT2) in bone marrow stromal cell autophagy and apoptosis. In: Stem Cell Research. 2015 ; Vol. 15, No. 2. pp. 312-321.
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