Abstract
Molecular diagnosis of disorders of follicle-stimulating hormone (FSH) production may become possible now that the gene for FSHβ has been characterized. Restriction-fragment length polymorphism (RFLP) analysis provides a means of organized search for molecular variants of FSH. The purpose of this study was to screen controls for the presence of RFLPs using the deoxyribonucleic acid (DNA) probe pFSHβ-1.4. Genomic DNA was digested with 12 different restriction endonucleases; Southern blots were constructed and hybridized to pFSHβ-1.4. No polymorphisms were identified with 11 enzymes. Three of 24 (12.6%) Hind III digests demonstrated a polymorphic fragment of either 5.2, 4.7, or 4.3 kb. These are the first RFLPs identified for the FSHβ gene with pFSHβ-1.4. RFLPs for FSHβ constitute the first step in the molecular analysis of disorders of FSH production.
Original language | English (US) |
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Pages (from-to) | 261-265 |
Number of pages | 5 |
Journal | Fertility and sterility |
Volume | 53 |
Issue number | 2 |
DOIs | |
State | Published - 1990 |
ASJC Scopus subject areas
- Reproductive Medicine
- Obstetrics and Gynecology