The detection of Hind III restriction-fragment length polymorphisms using a deoxyribonucleic acid probe for the beta subunit of follicle-stimulating hormone

Lawrence C Layman, D. J. Roach, L. Plouffe, Paul G McDonough, J. T. Wilson

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Molecular diagnosis of disorders of follicle-stimulating hormone (FSH) production may become possible now that the gene for FSHβ has been characterized. Restriction-fragment length polymorphism (RFLP) analysis provides a means of organized search for molecular variants of FSH. The purpose of this study was to screen controls for the presence of RFLPs using the deoxyribonucleic acid (DNA) probe pFSHβ-1.4. Genomic DNA was digested with 12 different restriction endonucleases; Southern blots were constructed and hybridized to pFSHβ-1.4. No polymorphisms were identified with 11 enzymes. Three of 24 (12.6%) Hind III digests demonstrated a polymorphic fragment of either 5.2, 4.7, or 4.3 kb. These are the first RFLPs identified for the FSHβ gene with pFSHβ-1.4. RFLPs for FSHβ constitute the first step in the molecular analysis of disorders of FSH production.

Original languageEnglish (US)
Pages (from-to)261-265
Number of pages5
JournalFertility and sterility
Volume53
Issue number2
DOIs
StatePublished - Jan 1 1990

Fingerprint

Beta Subunit Follicle Stimulating Hormone
Follicle Stimulating Hormone
Restriction Fragment Length Polymorphisms
DNA
DNA Restriction Enzymes
Southern Blotting
Genes
Enzymes

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

Cite this

The detection of Hind III restriction-fragment length polymorphisms using a deoxyribonucleic acid probe for the beta subunit of follicle-stimulating hormone. / Layman, Lawrence C; Roach, D. J.; Plouffe, L.; McDonough, Paul G; Wilson, J. T.

In: Fertility and sterility, Vol. 53, No. 2, 01.01.1990, p. 261-265.

Research output: Contribution to journalArticle

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