The Effect of Dietary Supplementation with n—3 Polyunsaturated Fatty Acids on the Synthesis of Interleukin-1 and Tumor Necrosis Factor by Mononuclear Cells

Stefan Endres, Reza Ghorbani, Vicki E. Kelley, Kostis Georgilis, Gerhard Lonnemann, Jos W.M. Van Der Meer, Joseph Gerard Cannon, Tina S. Rogers, Mark S. Klempner, Peter C. Weber, Ernst J. Schaefer, Sheldon M. Wolff, Charles A. Dinarello

Research output: Contribution to journalArticle

1590 Citations (Scopus)

Abstract

We examined whether the synthesis of interleukin-1 or tumor necrosis factor, two cytokines with potent inflammatory activities, is influenced by dietary supplementation with n—3 fatty acids. Nine healthy volunteers added 18 g of fish-oil concentrate per day to their normal Western diet for six weeks. We used a radioimmunoassay to measure interleukin-1 (IL-1β and IL-1α) and tumor necrosis factor produced in vitro by stimulated peripheral-blood mononuclear cells. With endotoxin as a stimulus, the synthesis of IL-1β was suppressed from 7.4±0.9 ng per milliliter at base line to 4.2±0.5 ng per milliliter after six weeks of supplementation (43 percent decrease; P = 0.048). Ten weeks after the end of n-3 supplementation, we observed a further decrease to 2.9±0.5 ng per milliliter (61 percent decrease; P = 0.005). The production of IL-1α and tumor necrosis factor responded in a similar manner. Twenty weeks after the end of supplementation, the production of IL-1βIL-1α, and tumor necrosis factor had returned to the presupplement level. The decreased production of interleukin-1 and tumor necrosis factor was accompanied by a decreased ratio of arachidonic acid to eicosapentaenoic acid in the membrane phospholipids of mononuclear cells. We conclude that the synthesis of IL-1β, IL-1α, and tumor necrosis factor can be suppressed by dietary supplementation with long-chain n—3 fatty acids. The reported antiinflammatory effect of these n—3 fatty acids may be mediated in part by their inhibitory effect on the production of interleukin-1 and tumor necrosis factor. INTERLEUKIN-1 and tumor necrosis factor are polypeptide cytokines synthesized by monocytes and other cells in response to injury, as well as to infectious, inflammatory, or immunologic challenges.1 2 3 These cytokines induce local inflammatory changes and mediate several systemic acute-phase responses.4,5 Moreover, interleukin-1 and tumor necrosis factor often act synergistically — e.g., on vascular endothelium,6 insulin production,7 and the synthesis of metabolites of arachidonic acid.8 The n—3 (also called omega—3) fatty acids are polyunsaturated fatty acids in which the last double bond is located between the third and fourth carbon atom from the methyl end of the fatty acid chain. These fatty…

Original languageEnglish (US)
Pages (from-to)265-271
Number of pages7
JournalNew England Journal of Medicine
Volume320
Issue number5
DOIs
StatePublished - Feb 2 1989

Fingerprint

Omega-3 Fatty Acids
Dietary Supplements
Interleukin-1
Tumor Necrosis Factor-alpha
Cytokines
Fatty Acids
Eicosapentaenoic Acid
Fish Oils
Vascular Endothelium
Unsaturated Fatty Acids
Arachidonic Acid
Endotoxins
Radioimmunoassay
Monocytes
Blood Cells
Phospholipids
Healthy Volunteers
Anti-Inflammatory Agents
Carbon
Insulin

ASJC Scopus subject areas

  • Medicine(all)

Cite this

The Effect of Dietary Supplementation with n—3 Polyunsaturated Fatty Acids on the Synthesis of Interleukin-1 and Tumor Necrosis Factor by Mononuclear Cells. / Endres, Stefan; Ghorbani, Reza; Kelley, Vicki E.; Georgilis, Kostis; Lonnemann, Gerhard; Van Der Meer, Jos W.M.; Cannon, Joseph Gerard; Rogers, Tina S.; Klempner, Mark S.; Weber, Peter C.; Schaefer, Ernst J.; Wolff, Sheldon M.; Dinarello, Charles A.

In: New England Journal of Medicine, Vol. 320, No. 5, 02.02.1989, p. 265-271.

Research output: Contribution to journalArticle

Endres, S, Ghorbani, R, Kelley, VE, Georgilis, K, Lonnemann, G, Van Der Meer, JWM, Cannon, JG, Rogers, TS, Klempner, MS, Weber, PC, Schaefer, EJ, Wolff, SM & Dinarello, CA 1989, 'The Effect of Dietary Supplementation with n—3 Polyunsaturated Fatty Acids on the Synthesis of Interleukin-1 and Tumor Necrosis Factor by Mononuclear Cells', New England Journal of Medicine, vol. 320, no. 5, pp. 265-271. https://doi.org/10.1056/NEJM198902023200501
Endres, Stefan ; Ghorbani, Reza ; Kelley, Vicki E. ; Georgilis, Kostis ; Lonnemann, Gerhard ; Van Der Meer, Jos W.M. ; Cannon, Joseph Gerard ; Rogers, Tina S. ; Klempner, Mark S. ; Weber, Peter C. ; Schaefer, Ernst J. ; Wolff, Sheldon M. ; Dinarello, Charles A. / The Effect of Dietary Supplementation with n—3 Polyunsaturated Fatty Acids on the Synthesis of Interleukin-1 and Tumor Necrosis Factor by Mononuclear Cells. In: New England Journal of Medicine. 1989 ; Vol. 320, No. 5. pp. 265-271.
@article{a849697592154d18986f614dedc7cc8b,
title = "The Effect of Dietary Supplementation with n—3 Polyunsaturated Fatty Acids on the Synthesis of Interleukin-1 and Tumor Necrosis Factor by Mononuclear Cells",
abstract = "We examined whether the synthesis of interleukin-1 or tumor necrosis factor, two cytokines with potent inflammatory activities, is influenced by dietary supplementation with n—3 fatty acids. Nine healthy volunteers added 18 g of fish-oil concentrate per day to their normal Western diet for six weeks. We used a radioimmunoassay to measure interleukin-1 (IL-1β and IL-1α) and tumor necrosis factor produced in vitro by stimulated peripheral-blood mononuclear cells. With endotoxin as a stimulus, the synthesis of IL-1β was suppressed from 7.4±0.9 ng per milliliter at base line to 4.2±0.5 ng per milliliter after six weeks of supplementation (43 percent decrease; P = 0.048). Ten weeks after the end of n-3 supplementation, we observed a further decrease to 2.9±0.5 ng per milliliter (61 percent decrease; P = 0.005). The production of IL-1α and tumor necrosis factor responded in a similar manner. Twenty weeks after the end of supplementation, the production of IL-1βIL-1α, and tumor necrosis factor had returned to the presupplement level. The decreased production of interleukin-1 and tumor necrosis factor was accompanied by a decreased ratio of arachidonic acid to eicosapentaenoic acid in the membrane phospholipids of mononuclear cells. We conclude that the synthesis of IL-1β, IL-1α, and tumor necrosis factor can be suppressed by dietary supplementation with long-chain n—3 fatty acids. The reported antiinflammatory effect of these n—3 fatty acids may be mediated in part by their inhibitory effect on the production of interleukin-1 and tumor necrosis factor. INTERLEUKIN-1 and tumor necrosis factor are polypeptide cytokines synthesized by monocytes and other cells in response to injury, as well as to infectious, inflammatory, or immunologic challenges.1 2 3 These cytokines induce local inflammatory changes and mediate several systemic acute-phase responses.4,5 Moreover, interleukin-1 and tumor necrosis factor often act synergistically — e.g., on vascular endothelium,6 insulin production,7 and the synthesis of metabolites of arachidonic acid.8 The n—3 (also called omega—3) fatty acids are polyunsaturated fatty acids in which the last double bond is located between the third and fourth carbon atom from the methyl end of the fatty acid chain. These fatty…",
author = "Stefan Endres and Reza Ghorbani and Kelley, {Vicki E.} and Kostis Georgilis and Gerhard Lonnemann and {Van Der Meer}, {Jos W.M.} and Cannon, {Joseph Gerard} and Rogers, {Tina S.} and Klempner, {Mark S.} and Weber, {Peter C.} and Schaefer, {Ernst J.} and Wolff, {Sheldon M.} and Dinarello, {Charles A.}",
year = "1989",
month = "2",
day = "2",
doi = "10.1056/NEJM198902023200501",
language = "English (US)",
volume = "320",
pages = "265--271",
journal = "New England Journal of Medicine",
issn = "0028-4793",
publisher = "Massachussetts Medical Society",
number = "5",

}

TY - JOUR

T1 - The Effect of Dietary Supplementation with n—3 Polyunsaturated Fatty Acids on the Synthesis of Interleukin-1 and Tumor Necrosis Factor by Mononuclear Cells

AU - Endres, Stefan

AU - Ghorbani, Reza

AU - Kelley, Vicki E.

AU - Georgilis, Kostis

AU - Lonnemann, Gerhard

AU - Van Der Meer, Jos W.M.

AU - Cannon, Joseph Gerard

AU - Rogers, Tina S.

AU - Klempner, Mark S.

AU - Weber, Peter C.

AU - Schaefer, Ernst J.

AU - Wolff, Sheldon M.

AU - Dinarello, Charles A.

PY - 1989/2/2

Y1 - 1989/2/2

N2 - We examined whether the synthesis of interleukin-1 or tumor necrosis factor, two cytokines with potent inflammatory activities, is influenced by dietary supplementation with n—3 fatty acids. Nine healthy volunteers added 18 g of fish-oil concentrate per day to their normal Western diet for six weeks. We used a radioimmunoassay to measure interleukin-1 (IL-1β and IL-1α) and tumor necrosis factor produced in vitro by stimulated peripheral-blood mononuclear cells. With endotoxin as a stimulus, the synthesis of IL-1β was suppressed from 7.4±0.9 ng per milliliter at base line to 4.2±0.5 ng per milliliter after six weeks of supplementation (43 percent decrease; P = 0.048). Ten weeks after the end of n-3 supplementation, we observed a further decrease to 2.9±0.5 ng per milliliter (61 percent decrease; P = 0.005). The production of IL-1α and tumor necrosis factor responded in a similar manner. Twenty weeks after the end of supplementation, the production of IL-1βIL-1α, and tumor necrosis factor had returned to the presupplement level. The decreased production of interleukin-1 and tumor necrosis factor was accompanied by a decreased ratio of arachidonic acid to eicosapentaenoic acid in the membrane phospholipids of mononuclear cells. We conclude that the synthesis of IL-1β, IL-1α, and tumor necrosis factor can be suppressed by dietary supplementation with long-chain n—3 fatty acids. The reported antiinflammatory effect of these n—3 fatty acids may be mediated in part by their inhibitory effect on the production of interleukin-1 and tumor necrosis factor. INTERLEUKIN-1 and tumor necrosis factor are polypeptide cytokines synthesized by monocytes and other cells in response to injury, as well as to infectious, inflammatory, or immunologic challenges.1 2 3 These cytokines induce local inflammatory changes and mediate several systemic acute-phase responses.4,5 Moreover, interleukin-1 and tumor necrosis factor often act synergistically — e.g., on vascular endothelium,6 insulin production,7 and the synthesis of metabolites of arachidonic acid.8 The n—3 (also called omega—3) fatty acids are polyunsaturated fatty acids in which the last double bond is located between the third and fourth carbon atom from the methyl end of the fatty acid chain. These fatty…

AB - We examined whether the synthesis of interleukin-1 or tumor necrosis factor, two cytokines with potent inflammatory activities, is influenced by dietary supplementation with n—3 fatty acids. Nine healthy volunteers added 18 g of fish-oil concentrate per day to their normal Western diet for six weeks. We used a radioimmunoassay to measure interleukin-1 (IL-1β and IL-1α) and tumor necrosis factor produced in vitro by stimulated peripheral-blood mononuclear cells. With endotoxin as a stimulus, the synthesis of IL-1β was suppressed from 7.4±0.9 ng per milliliter at base line to 4.2±0.5 ng per milliliter after six weeks of supplementation (43 percent decrease; P = 0.048). Ten weeks after the end of n-3 supplementation, we observed a further decrease to 2.9±0.5 ng per milliliter (61 percent decrease; P = 0.005). The production of IL-1α and tumor necrosis factor responded in a similar manner. Twenty weeks after the end of supplementation, the production of IL-1βIL-1α, and tumor necrosis factor had returned to the presupplement level. The decreased production of interleukin-1 and tumor necrosis factor was accompanied by a decreased ratio of arachidonic acid to eicosapentaenoic acid in the membrane phospholipids of mononuclear cells. We conclude that the synthesis of IL-1β, IL-1α, and tumor necrosis factor can be suppressed by dietary supplementation with long-chain n—3 fatty acids. The reported antiinflammatory effect of these n—3 fatty acids may be mediated in part by their inhibitory effect on the production of interleukin-1 and tumor necrosis factor. INTERLEUKIN-1 and tumor necrosis factor are polypeptide cytokines synthesized by monocytes and other cells in response to injury, as well as to infectious, inflammatory, or immunologic challenges.1 2 3 These cytokines induce local inflammatory changes and mediate several systemic acute-phase responses.4,5 Moreover, interleukin-1 and tumor necrosis factor often act synergistically — e.g., on vascular endothelium,6 insulin production,7 and the synthesis of metabolites of arachidonic acid.8 The n—3 (also called omega—3) fatty acids are polyunsaturated fatty acids in which the last double bond is located between the third and fourth carbon atom from the methyl end of the fatty acid chain. These fatty…

UR - http://www.scopus.com/inward/record.url?scp=0024489402&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024489402&partnerID=8YFLogxK

U2 - 10.1056/NEJM198902023200501

DO - 10.1056/NEJM198902023200501

M3 - Article

C2 - 2783477

AN - SCOPUS:0024489402

VL - 320

SP - 265

EP - 271

JO - New England Journal of Medicine

JF - New England Journal of Medicine

SN - 0028-4793

IS - 5

ER -