The effect of estradiol on the expression of estrogen, progesterone, androgen, and prolactin receptors in human peritoneal fibroblasts

Laura Detti, Ghassan M. Saed, Zhong L. Jiang, Michael L. Kruger, Michael Peter Diamond

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Purpose: To assess the ability of fibroblasts isolated from normal peritoneum and adhesion tissues to express various hormone receptors when cultured with exogenous estradiol. Methods: Primary cultures of fibroblasts from normal human peritoneum and adhesion tissue were treated with zero (control), 10-10, 10-8, and 10-6 M concentrations of 17β-estradiol. We performed real time reverse transcriptase polymerase chain reaction to determine mRNA levels of estradiol-α receptor (ER-α) and estradiol-β receptor (ER-β), progesterone receptor (P-R), androgen receptor (A-R), and prolactin receptor (PRL-R) in the two types of fibroblast cultures. Results: In the control groups, P-R and A-R were higher in normal than in adhesion fibroblasts. In adhesion cells, ER-α were higher at 10-8 estradiol; ER-β were higher at 10-6 M estradiol; P-R remained constant; A-R showed a higher expression at 10 -10 and 10-8 M estradiol; and PRL-R showed an exponential increase at 10-10 M estradiol. Conclusions: The inflammatory-like changes manifested by adhesion fibroblasts enhance the anabolic hormones receptor expression (ER-α, ER-β, PRL, and A-R), when exposed to estradiol.

Original languageEnglish (US)
Pages (from-to)245-250
Number of pages6
JournalJournal of Assisted Reproduction and Genetics
Volume25
Issue number6
DOIs
StatePublished - Jun 1 2008

Fingerprint

Estradiol Receptors
Prolactin Receptors
Androgen Receptors
Progesterone Receptors
Estrogen Receptors
Estradiol
Fibroblasts
Tissue Adhesions
Peritoneum
Hormones
Reverse Transcriptase Polymerase Chain Reaction
Cell Adhesion
Real-Time Polymerase Chain Reaction
Control Groups
Messenger RNA

Keywords

  • Adhesions
  • Ex-vivo study
  • Fibroblasts
  • Hormone receptors
  • Ovulation induction

ASJC Scopus subject areas

  • Reproductive Medicine
  • Genetics
  • Obstetrics and Gynecology
  • Developmental Biology
  • Genetics(clinical)

Cite this

The effect of estradiol on the expression of estrogen, progesterone, androgen, and prolactin receptors in human peritoneal fibroblasts. / Detti, Laura; Saed, Ghassan M.; Jiang, Zhong L.; Kruger, Michael L.; Diamond, Michael Peter.

In: Journal of Assisted Reproduction and Genetics, Vol. 25, No. 6, 01.06.2008, p. 245-250.

Research output: Contribution to journalArticle

@article{49cf0711dfcc4fada6a40de45ad1928c,
title = "The effect of estradiol on the expression of estrogen, progesterone, androgen, and prolactin receptors in human peritoneal fibroblasts",
abstract = "Purpose: To assess the ability of fibroblasts isolated from normal peritoneum and adhesion tissues to express various hormone receptors when cultured with exogenous estradiol. Methods: Primary cultures of fibroblasts from normal human peritoneum and adhesion tissue were treated with zero (control), 10-10, 10-8, and 10-6 M concentrations of 17β-estradiol. We performed real time reverse transcriptase polymerase chain reaction to determine mRNA levels of estradiol-α receptor (ER-α) and estradiol-β receptor (ER-β), progesterone receptor (P-R), androgen receptor (A-R), and prolactin receptor (PRL-R) in the two types of fibroblast cultures. Results: In the control groups, P-R and A-R were higher in normal than in adhesion fibroblasts. In adhesion cells, ER-α were higher at 10-8 estradiol; ER-β were higher at 10-6 M estradiol; P-R remained constant; A-R showed a higher expression at 10 -10 and 10-8 M estradiol; and PRL-R showed an exponential increase at 10-10 M estradiol. Conclusions: The inflammatory-like changes manifested by adhesion fibroblasts enhance the anabolic hormones receptor expression (ER-α, ER-β, PRL, and A-R), when exposed to estradiol.",
keywords = "Adhesions, Ex-vivo study, Fibroblasts, Hormone receptors, Ovulation induction",
author = "Laura Detti and Saed, {Ghassan M.} and Jiang, {Zhong L.} and Kruger, {Michael L.} and Diamond, {Michael Peter}",
year = "2008",
month = "6",
day = "1",
doi = "10.1007/s10815-008-9230-5",
language = "English (US)",
volume = "25",
pages = "245--250",
journal = "Journal of Assisted Reproduction and Genetics",
issn = "1058-0468",
publisher = "Springer New York",
number = "6",

}

TY - JOUR

T1 - The effect of estradiol on the expression of estrogen, progesterone, androgen, and prolactin receptors in human peritoneal fibroblasts

AU - Detti, Laura

AU - Saed, Ghassan M.

AU - Jiang, Zhong L.

AU - Kruger, Michael L.

AU - Diamond, Michael Peter

PY - 2008/6/1

Y1 - 2008/6/1

N2 - Purpose: To assess the ability of fibroblasts isolated from normal peritoneum and adhesion tissues to express various hormone receptors when cultured with exogenous estradiol. Methods: Primary cultures of fibroblasts from normal human peritoneum and adhesion tissue were treated with zero (control), 10-10, 10-8, and 10-6 M concentrations of 17β-estradiol. We performed real time reverse transcriptase polymerase chain reaction to determine mRNA levels of estradiol-α receptor (ER-α) and estradiol-β receptor (ER-β), progesterone receptor (P-R), androgen receptor (A-R), and prolactin receptor (PRL-R) in the two types of fibroblast cultures. Results: In the control groups, P-R and A-R were higher in normal than in adhesion fibroblasts. In adhesion cells, ER-α were higher at 10-8 estradiol; ER-β were higher at 10-6 M estradiol; P-R remained constant; A-R showed a higher expression at 10 -10 and 10-8 M estradiol; and PRL-R showed an exponential increase at 10-10 M estradiol. Conclusions: The inflammatory-like changes manifested by adhesion fibroblasts enhance the anabolic hormones receptor expression (ER-α, ER-β, PRL, and A-R), when exposed to estradiol.

AB - Purpose: To assess the ability of fibroblasts isolated from normal peritoneum and adhesion tissues to express various hormone receptors when cultured with exogenous estradiol. Methods: Primary cultures of fibroblasts from normal human peritoneum and adhesion tissue were treated with zero (control), 10-10, 10-8, and 10-6 M concentrations of 17β-estradiol. We performed real time reverse transcriptase polymerase chain reaction to determine mRNA levels of estradiol-α receptor (ER-α) and estradiol-β receptor (ER-β), progesterone receptor (P-R), androgen receptor (A-R), and prolactin receptor (PRL-R) in the two types of fibroblast cultures. Results: In the control groups, P-R and A-R were higher in normal than in adhesion fibroblasts. In adhesion cells, ER-α were higher at 10-8 estradiol; ER-β were higher at 10-6 M estradiol; P-R remained constant; A-R showed a higher expression at 10 -10 and 10-8 M estradiol; and PRL-R showed an exponential increase at 10-10 M estradiol. Conclusions: The inflammatory-like changes manifested by adhesion fibroblasts enhance the anabolic hormones receptor expression (ER-α, ER-β, PRL, and A-R), when exposed to estradiol.

KW - Adhesions

KW - Ex-vivo study

KW - Fibroblasts

KW - Hormone receptors

KW - Ovulation induction

UR - http://www.scopus.com/inward/record.url?scp=48349130666&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=48349130666&partnerID=8YFLogxK

U2 - 10.1007/s10815-008-9230-5

DO - 10.1007/s10815-008-9230-5

M3 - Article

C2 - 18551363

AN - SCOPUS:48349130666

VL - 25

SP - 245

EP - 250

JO - Journal of Assisted Reproduction and Genetics

JF - Journal of Assisted Reproduction and Genetics

SN - 1058-0468

IS - 6

ER -