The effect of fluoridated and non-fluoridated rewetting agents on in vitro recurrent caries

Anut Itthagarun, Nigel M. King, James S. Wefel, Franklin Chi Meng Tay, David Henry Pashley

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Objective: This study examined the in vitro caries inhibiting potential of fluoridated and non-fluoridated rewetting agents that are applied to acid-etched enamel and dentine before the use of a water-free, dentine adhesive. Materials and methods: Twelve caries-free premolars were divided into three groups of four teeth each. 2 × 3 × 1.5 mm cavities were prepared on the mesial and distal surfaces of each tooth, with half of the cavosurface margin in enamel and half in root dentine. In Group I (control), One-Step (Bisco, Schaumburg, USA) was applied without etching or rewetting agents. In Group II, cavities were acid-etched, rinsed, dried, and rewetted with Aqua-Prep (Bisco), a non-fluoridated rewetting agent, and then bonded with One-Step. Treatment for Group III was similar to Group II, except that Aqua-Prep F (Bisco), a fluoridated rewetting agent was used. Bonded cavities were restored with a non-fluoride-containing flowable composite (ÆliteFlo, Bisco). Artificial carious lesions were induced in these specimens, from which multiple 100 ± 20 μm thick longitudinal sections were prepared, yielding 16 specimens per group for evaluation with polarised light microscopy (PLM) and microradiography (MRG). Representative sections were processed for transmission electron microscopy (TEM) examination and scanning transmission electron microscopy/energy dispersive X-ray (STEM/EDX) analyses. Results: The differences in demineralisation of dentine among the groups were not statistically significant for 'relative' lesion depth (p > 0.05, ANOVA, Student-Neuman-Keuls test), but highly significant for 'relative' lesion area (p < 0.001). Wall lesions were consistently present in Group I, while inhibition zones were invariably observed in Group III. 87.5% of Group II specimens exhibited neither wall lesion nor inhibition zone. TEM showed that remnant dentine apatite crystallites within the inhibition zones in Group III were larger and denser than those present within the corresponding wall lesions. STEM/EDX analyses confirmed the presence of calcium, phosphorus and fluorine in these plate-like crystallites. Conclusion: When used with a water-free, single-bottle dentine adhesive, a non-fluoridated rewetting agent is able to reduce, but cannot completely prevent recurrent caries. The use of a fluoridated rewetting agent is useful under the situation when microleakage occurs, by providing the additional benefit of fluoride-induced demineralisation inhibition.

Original languageEnglish (US)
Pages (from-to)255-273
Number of pages19
JournalJournal of Dentistry
Volume29
Issue number4
DOIs
StatePublished - May 1 2001

Fingerprint

Dentin
Scanning Transmission Electron Microscopy
Dental Enamel
Transmission Electron Microscopy
Adhesives
Tooth
X-Rays
Microradiography
Polarization Microscopy
Apatites
Acids
Fluorine
Water
Bicuspid
Fluorides
Phosphorus
In Vitro Techniques
Analysis of Variance
Students
Calcium

Keywords

  • Fluoride rewetting agent
  • Microradiography
  • Polarised light microscopy
  • Recurrent caries
  • STEM/EDX
  • TEM

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

The effect of fluoridated and non-fluoridated rewetting agents on in vitro recurrent caries. / Itthagarun, Anut; King, Nigel M.; Wefel, James S.; Tay, Franklin Chi Meng; Pashley, David Henry.

In: Journal of Dentistry, Vol. 29, No. 4, 01.05.2001, p. 255-273.

Research output: Contribution to journalArticle

Itthagarun, Anut ; King, Nigel M. ; Wefel, James S. ; Tay, Franklin Chi Meng ; Pashley, David Henry. / The effect of fluoridated and non-fluoridated rewetting agents on in vitro recurrent caries. In: Journal of Dentistry. 2001 ; Vol. 29, No. 4. pp. 255-273.
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AU - Pashley, David Henry

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N2 - Objective: This study examined the in vitro caries inhibiting potential of fluoridated and non-fluoridated rewetting agents that are applied to acid-etched enamel and dentine before the use of a water-free, dentine adhesive. Materials and methods: Twelve caries-free premolars were divided into three groups of four teeth each. 2 × 3 × 1.5 mm cavities were prepared on the mesial and distal surfaces of each tooth, with half of the cavosurface margin in enamel and half in root dentine. In Group I (control), One-Step (Bisco, Schaumburg, USA) was applied without etching or rewetting agents. In Group II, cavities were acid-etched, rinsed, dried, and rewetted with Aqua-Prep (Bisco), a non-fluoridated rewetting agent, and then bonded with One-Step. Treatment for Group III was similar to Group II, except that Aqua-Prep F (Bisco), a fluoridated rewetting agent was used. Bonded cavities were restored with a non-fluoride-containing flowable composite (ÆliteFlo, Bisco). Artificial carious lesions were induced in these specimens, from which multiple 100 ± 20 μm thick longitudinal sections were prepared, yielding 16 specimens per group for evaluation with polarised light microscopy (PLM) and microradiography (MRG). Representative sections were processed for transmission electron microscopy (TEM) examination and scanning transmission electron microscopy/energy dispersive X-ray (STEM/EDX) analyses. Results: The differences in demineralisation of dentine among the groups were not statistically significant for 'relative' lesion depth (p > 0.05, ANOVA, Student-Neuman-Keuls test), but highly significant for 'relative' lesion area (p < 0.001). Wall lesions were consistently present in Group I, while inhibition zones were invariably observed in Group III. 87.5% of Group II specimens exhibited neither wall lesion nor inhibition zone. TEM showed that remnant dentine apatite crystallites within the inhibition zones in Group III were larger and denser than those present within the corresponding wall lesions. STEM/EDX analyses confirmed the presence of calcium, phosphorus and fluorine in these plate-like crystallites. Conclusion: When used with a water-free, single-bottle dentine adhesive, a non-fluoridated rewetting agent is able to reduce, but cannot completely prevent recurrent caries. The use of a fluoridated rewetting agent is useful under the situation when microleakage occurs, by providing the additional benefit of fluoride-induced demineralisation inhibition.

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KW - Microradiography

KW - Polarised light microscopy

KW - Recurrent caries

KW - STEM/EDX

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