The effectiveness of a pre-procedural mouthrinse in reducing bacteria on radiographic phosphor plates

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Purpose: This study assessed the effectiveness of three antimicrobial mouthrinses in reducing microbial growth on photostimulable phosphor (PSP) plates. Materials and Methods: Prior to performing a full-mouth radiographic survey (FMX), subjects were asked to rinse with one of the three test rinses (Listerine®, Decapinol®, or chlorhexidine oral rinse 0.12%) or to refrain from rinsing. Four PSP plates were sampled from each FMX through collection into sterile containers upon exiting the scanner. Flame-sterilized forceps were used to transfer the PSP plates onto blood agar plates (5% sheep blood agar). The blood agar plates were incubated at 37oC for up to 72 h. An environmental control blood agar plate was incubated with each batch. Additionally, for control, 25 gas-sterilized PSP plates were plated onto blood agar and analyzed. Results: The mean number of bacterial colonies per plate was the lowest in the chlorhexidine group, followed by the Decapinol, Listerine, and the no rinse negative control groups. Only the chlorhexidine and Listerine groups were significantly different (p=0.005). No growth was observed for the 25 gas-sterilized control plates or the environmental control blood agar plates. Conclusion: The mean number of bacterial colonies was the lowest in the chlorhexidine group, followed by the Decapinol, Listerine, and the no rinse groups. Nonetheless, a statistically significant difference was found only in the case of Listerine. Additional research is needed to test whether a higher concentration (0.2%) or longer exposure period (two consecutive 30 s rinse periods) would be helpful in reducing PSP plate contamination further with chlorhexidine.

Original languageEnglish (US)
Pages (from-to)149-154
Number of pages6
JournalImaging Science in Dentistry
Volume44
Issue number2
DOIs
StatePublished - Jan 1 2014

Fingerprint

Chlorhexidine
Agar
Bacteria
Gases
Growth
Surgical Instruments
Mouth
Sheep
Listerine
Control Groups
Research

Keywords

  • Chlorhexidine
  • Delmopinol
  • Diagnostic imaging
  • Listerine

ASJC Scopus subject areas

  • Dentistry(all)
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

The effectiveness of a pre-procedural mouthrinse in reducing bacteria on radiographic phosphor plates. / Buchanan, Allison K; Kalathingal, Sajitha Menon; Shrout, Michael; Plummer, Kevin D; Looney, Stephen Warwick.

In: Imaging Science in Dentistry, Vol. 44, No. 2, 01.01.2014, p. 149-154.

Research output: Contribution to journalArticle

@article{8f282f0d3e7f4df08988fdb44965652e,
title = "The effectiveness of a pre-procedural mouthrinse in reducing bacteria on radiographic phosphor plates",
abstract = "Purpose: This study assessed the effectiveness of three antimicrobial mouthrinses in reducing microbial growth on photostimulable phosphor (PSP) plates. Materials and Methods: Prior to performing a full-mouth radiographic survey (FMX), subjects were asked to rinse with one of the three test rinses (Listerine{\circledR}, Decapinol{\circledR}, or chlorhexidine oral rinse 0.12{\%}) or to refrain from rinsing. Four PSP plates were sampled from each FMX through collection into sterile containers upon exiting the scanner. Flame-sterilized forceps were used to transfer the PSP plates onto blood agar plates (5{\%} sheep blood agar). The blood agar plates were incubated at 37oC for up to 72 h. An environmental control blood agar plate was incubated with each batch. Additionally, for control, 25 gas-sterilized PSP plates were plated onto blood agar and analyzed. Results: The mean number of bacterial colonies per plate was the lowest in the chlorhexidine group, followed by the Decapinol, Listerine, and the no rinse negative control groups. Only the chlorhexidine and Listerine groups were significantly different (p=0.005). No growth was observed for the 25 gas-sterilized control plates or the environmental control blood agar plates. Conclusion: The mean number of bacterial colonies was the lowest in the chlorhexidine group, followed by the Decapinol, Listerine, and the no rinse groups. Nonetheless, a statistically significant difference was found only in the case of Listerine. Additional research is needed to test whether a higher concentration (0.2{\%}) or longer exposure period (two consecutive 30 s rinse periods) would be helpful in reducing PSP plate contamination further with chlorhexidine.",
keywords = "Chlorhexidine, Delmopinol, Diagnostic imaging, Listerine",
author = "Buchanan, {Allison K} and Kalathingal, {Sajitha Menon} and Michael Shrout and Plummer, {Kevin D} and Looney, {Stephen Warwick}",
year = "2014",
month = "1",
day = "1",
doi = "10.5624/isd.2014.44.2.149",
language = "English (US)",
volume = "44",
pages = "149--154",
journal = "Imaging Science in Dentistry",
issn = "2233-7822",
publisher = "Korean Academy of Oral and Maxillofacial Radiology",
number = "2",

}

TY - JOUR

T1 - The effectiveness of a pre-procedural mouthrinse in reducing bacteria on radiographic phosphor plates

AU - Buchanan, Allison K

AU - Kalathingal, Sajitha Menon

AU - Shrout, Michael

AU - Plummer, Kevin D

AU - Looney, Stephen Warwick

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Purpose: This study assessed the effectiveness of three antimicrobial mouthrinses in reducing microbial growth on photostimulable phosphor (PSP) plates. Materials and Methods: Prior to performing a full-mouth radiographic survey (FMX), subjects were asked to rinse with one of the three test rinses (Listerine®, Decapinol®, or chlorhexidine oral rinse 0.12%) or to refrain from rinsing. Four PSP plates were sampled from each FMX through collection into sterile containers upon exiting the scanner. Flame-sterilized forceps were used to transfer the PSP plates onto blood agar plates (5% sheep blood agar). The blood agar plates were incubated at 37oC for up to 72 h. An environmental control blood agar plate was incubated with each batch. Additionally, for control, 25 gas-sterilized PSP plates were plated onto blood agar and analyzed. Results: The mean number of bacterial colonies per plate was the lowest in the chlorhexidine group, followed by the Decapinol, Listerine, and the no rinse negative control groups. Only the chlorhexidine and Listerine groups were significantly different (p=0.005). No growth was observed for the 25 gas-sterilized control plates or the environmental control blood agar plates. Conclusion: The mean number of bacterial colonies was the lowest in the chlorhexidine group, followed by the Decapinol, Listerine, and the no rinse groups. Nonetheless, a statistically significant difference was found only in the case of Listerine. Additional research is needed to test whether a higher concentration (0.2%) or longer exposure period (two consecutive 30 s rinse periods) would be helpful in reducing PSP plate contamination further with chlorhexidine.

AB - Purpose: This study assessed the effectiveness of three antimicrobial mouthrinses in reducing microbial growth on photostimulable phosphor (PSP) plates. Materials and Methods: Prior to performing a full-mouth radiographic survey (FMX), subjects were asked to rinse with one of the three test rinses (Listerine®, Decapinol®, or chlorhexidine oral rinse 0.12%) or to refrain from rinsing. Four PSP plates were sampled from each FMX through collection into sterile containers upon exiting the scanner. Flame-sterilized forceps were used to transfer the PSP plates onto blood agar plates (5% sheep blood agar). The blood agar plates were incubated at 37oC for up to 72 h. An environmental control blood agar plate was incubated with each batch. Additionally, for control, 25 gas-sterilized PSP plates were plated onto blood agar and analyzed. Results: The mean number of bacterial colonies per plate was the lowest in the chlorhexidine group, followed by the Decapinol, Listerine, and the no rinse negative control groups. Only the chlorhexidine and Listerine groups were significantly different (p=0.005). No growth was observed for the 25 gas-sterilized control plates or the environmental control blood agar plates. Conclusion: The mean number of bacterial colonies was the lowest in the chlorhexidine group, followed by the Decapinol, Listerine, and the no rinse groups. Nonetheless, a statistically significant difference was found only in the case of Listerine. Additional research is needed to test whether a higher concentration (0.2%) or longer exposure period (two consecutive 30 s rinse periods) would be helpful in reducing PSP plate contamination further with chlorhexidine.

KW - Chlorhexidine

KW - Delmopinol

KW - Diagnostic imaging

KW - Listerine

UR - http://www.scopus.com/inward/record.url?scp=84902457230&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84902457230&partnerID=8YFLogxK

U2 - 10.5624/isd.2014.44.2.149

DO - 10.5624/isd.2014.44.2.149

M3 - Article

AN - SCOPUS:84902457230

VL - 44

SP - 149

EP - 154

JO - Imaging Science in Dentistry

JF - Imaging Science in Dentistry

SN - 2233-7822

IS - 2

ER -