The effects of hyperthermia on tumor carcinoembryonic antigen expression

Jeffrey Y.C. Wong, Nahid F Mivechi, Raymond J. Paxton, Lawrence E. Williams, Barbara G. Beatty, J. David Beatty, John E. Shively

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The effects of hyperthermia on carcinoembryonic antigen (CEA) expression were investigated. The human colon adenocarcinoma cell line, LS174T, was heated in vitro for 42°C/1 hr, 43°C/1 hr, or 45°C/30 min. Carcinoembryonic antigen membrane expression was assayed by live cell radioimmunoassay 0--6 days after heating. A heat exposure of 45°C/30 min resulted in an initial decrease in carcinoembryonic antigen membrane expression 1 day post-heating followed by a 2-3 fold increase which peaked 3 days post-heating. Carcinoembryonic antigen expression began returning to normal by the sixth day. Heat exposures of 42°C/1 hr and 43°C/1 hr also resulted in increased carcinoembryonic antigen expression but this increase was of lesser magnitude and of shorter duration. Carcinoembryonic antigen shed into the medium, as measured by enzyme immunoassay, also increased after heating in a temperature-dependent fashion. Flow cytometry analysis demonstrated that cells in all phases of the cell cycle expressed this increase. We conclude that hyperthermia results in significant changes in carcinoembryonic antigen membrane expression and shedding. The implications that these findings have with regards to clinical hyperthermia and radioimmunotherapy are discussed.

Original languageEnglish (US)
Pages (from-to)803-808
Number of pages6
JournalInternational Journal of Radiation Oncology, Biology, Physics
Volume17
Issue number4
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

Fingerprint

hyperthermia
Carcinoembryonic Antigen
Neoplasm Antigens
antigens
Fever
tumors
Heating
heating
membranes
Membranes
Hot Temperature
radioimmunoassay
Radioimmunotherapy
sheds
heat
immunoassay
cytometry
cells
Immunoenzyme Techniques
cultured cells

Keywords

  • Carcinoembryonic antigen
  • Colon cancer
  • Hyperthermia

ASJC Scopus subject areas

  • Radiation
  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Cancer Research

Cite this

The effects of hyperthermia on tumor carcinoembryonic antigen expression. / Wong, Jeffrey Y.C.; Mivechi, Nahid F; Paxton, Raymond J.; Williams, Lawrence E.; Beatty, Barbara G.; Beatty, J. David; Shively, John E.

In: International Journal of Radiation Oncology, Biology, Physics, Vol. 17, No. 4, 01.01.1989, p. 803-808.

Research output: Contribution to journalArticle

Wong, Jeffrey Y.C. ; Mivechi, Nahid F ; Paxton, Raymond J. ; Williams, Lawrence E. ; Beatty, Barbara G. ; Beatty, J. David ; Shively, John E. / The effects of hyperthermia on tumor carcinoembryonic antigen expression. In: International Journal of Radiation Oncology, Biology, Physics. 1989 ; Vol. 17, No. 4. pp. 803-808.
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