The inhibitory effect of polyvinylphosphonic acid on functional matrix metalloproteinase activities in human demineralized dentin

Arzu Tezvergil-Mutluay, Kelli A. Agee, Tomohiro Hoshika, Franklin Chi Meng Tay, David Henry Pashley

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

This study has examined the use of polyvinylphosphonic acid (PVPA) as a potential matrix metalloproteinase (MMP) inhibitor and how brief cross-linking of demineralized dentin matrix that did not affect its mechanical properties enhanced the anti-MMP activity of PVPA. The anti-MMP potential of five PVPA concentrations (100-3000 μg ml-1) was initially screened using a rhMMP-9 colorimetic assay. Demineralized dentin beams were treated with the same five concentrations of PVPA to collagen and then aged for 30 days in a calcium- and zinc-containing medium. The changes in modulus of elasticity, loss of dry mass and dissolution of collagen peptides were measured via three-point bending, precision weighing and hydroxyproline assay, respectively. All tested PVPA concentrations were highly effective (P < 0.05) in inhibiting MMP-9. Ageing in the incubation medium did not significantly alter the modulus of elasticity of the five PVPA treatment groups. Conversely, aged dentin beams from the control group exhibited a significant decline in their modulus of elasticity (P < 0.05) over time. Mass loss from the dentin beams and the corresponding increase in hydroxyproline in the medium in the five PVPA treatment groups were significantly lower than for the control (P < 0.05). PVPA is a potent inhibitor of endogenous MMP activities in demineralized dentin. It may be used as an alternative to chlorhexidine to prevent collagen degradation within hybrid layers to extend the longevity of resin-dentin bonds.

Original languageEnglish (US)
Pages (from-to)4136-4142
Number of pages7
JournalActa biomaterialia
Volume6
Issue number10
DOIs
StatePublished - Oct 1 2010

Fingerprint

Dentin
Matrix Metalloproteinases
Human Activities
Acids
Elastic Modulus
Collagen
Hydroxyproline
Matrix Metalloproteinase Inhibitors
Elastic moduli
Assays
Chlorhexidine
Metalloproteases
Matrix Metalloproteinase 9
Weighing
Peptides
Zinc
Calcium
Dissolution
Resins
Aging of materials

Keywords

  • Acid etch
  • Dentin
  • Hydroxyproline
  • Inhibitor
  • Matrix metalloproteinase

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology

Cite this

The inhibitory effect of polyvinylphosphonic acid on functional matrix metalloproteinase activities in human demineralized dentin. / Tezvergil-Mutluay, Arzu; Agee, Kelli A.; Hoshika, Tomohiro; Tay, Franklin Chi Meng; Pashley, David Henry.

In: Acta biomaterialia, Vol. 6, No. 10, 01.10.2010, p. 4136-4142.

Research output: Contribution to journalArticle

Tezvergil-Mutluay, Arzu ; Agee, Kelli A. ; Hoshika, Tomohiro ; Tay, Franklin Chi Meng ; Pashley, David Henry. / The inhibitory effect of polyvinylphosphonic acid on functional matrix metalloproteinase activities in human demineralized dentin. In: Acta biomaterialia. 2010 ; Vol. 6, No. 10. pp. 4136-4142.
@article{666a06a00539404098168ce164fc9de3,
title = "The inhibitory effect of polyvinylphosphonic acid on functional matrix metalloproteinase activities in human demineralized dentin",
abstract = "This study has examined the use of polyvinylphosphonic acid (PVPA) as a potential matrix metalloproteinase (MMP) inhibitor and how brief cross-linking of demineralized dentin matrix that did not affect its mechanical properties enhanced the anti-MMP activity of PVPA. The anti-MMP potential of five PVPA concentrations (100-3000 μg ml-1) was initially screened using a rhMMP-9 colorimetic assay. Demineralized dentin beams were treated with the same five concentrations of PVPA to collagen and then aged for 30 days in a calcium- and zinc-containing medium. The changes in modulus of elasticity, loss of dry mass and dissolution of collagen peptides were measured via three-point bending, precision weighing and hydroxyproline assay, respectively. All tested PVPA concentrations were highly effective (P < 0.05) in inhibiting MMP-9. Ageing in the incubation medium did not significantly alter the modulus of elasticity of the five PVPA treatment groups. Conversely, aged dentin beams from the control group exhibited a significant decline in their modulus of elasticity (P < 0.05) over time. Mass loss from the dentin beams and the corresponding increase in hydroxyproline in the medium in the five PVPA treatment groups were significantly lower than for the control (P < 0.05). PVPA is a potent inhibitor of endogenous MMP activities in demineralized dentin. It may be used as an alternative to chlorhexidine to prevent collagen degradation within hybrid layers to extend the longevity of resin-dentin bonds.",
keywords = "Acid etch, Dentin, Hydroxyproline, Inhibitor, Matrix metalloproteinase",
author = "Arzu Tezvergil-Mutluay and Agee, {Kelli A.} and Tomohiro Hoshika and Tay, {Franklin Chi Meng} and Pashley, {David Henry}",
year = "2010",
month = "10",
day = "1",
doi = "10.1016/j.actbio.2010.05.017",
language = "English (US)",
volume = "6",
pages = "4136--4142",
journal = "Acta Biomaterialia",
issn = "1742-7061",
publisher = "Elsevier BV",
number = "10",

}

TY - JOUR

T1 - The inhibitory effect of polyvinylphosphonic acid on functional matrix metalloproteinase activities in human demineralized dentin

AU - Tezvergil-Mutluay, Arzu

AU - Agee, Kelli A.

AU - Hoshika, Tomohiro

AU - Tay, Franklin Chi Meng

AU - Pashley, David Henry

PY - 2010/10/1

Y1 - 2010/10/1

N2 - This study has examined the use of polyvinylphosphonic acid (PVPA) as a potential matrix metalloproteinase (MMP) inhibitor and how brief cross-linking of demineralized dentin matrix that did not affect its mechanical properties enhanced the anti-MMP activity of PVPA. The anti-MMP potential of five PVPA concentrations (100-3000 μg ml-1) was initially screened using a rhMMP-9 colorimetic assay. Demineralized dentin beams were treated with the same five concentrations of PVPA to collagen and then aged for 30 days in a calcium- and zinc-containing medium. The changes in modulus of elasticity, loss of dry mass and dissolution of collagen peptides were measured via three-point bending, precision weighing and hydroxyproline assay, respectively. All tested PVPA concentrations were highly effective (P < 0.05) in inhibiting MMP-9. Ageing in the incubation medium did not significantly alter the modulus of elasticity of the five PVPA treatment groups. Conversely, aged dentin beams from the control group exhibited a significant decline in their modulus of elasticity (P < 0.05) over time. Mass loss from the dentin beams and the corresponding increase in hydroxyproline in the medium in the five PVPA treatment groups were significantly lower than for the control (P < 0.05). PVPA is a potent inhibitor of endogenous MMP activities in demineralized dentin. It may be used as an alternative to chlorhexidine to prevent collagen degradation within hybrid layers to extend the longevity of resin-dentin bonds.

AB - This study has examined the use of polyvinylphosphonic acid (PVPA) as a potential matrix metalloproteinase (MMP) inhibitor and how brief cross-linking of demineralized dentin matrix that did not affect its mechanical properties enhanced the anti-MMP activity of PVPA. The anti-MMP potential of five PVPA concentrations (100-3000 μg ml-1) was initially screened using a rhMMP-9 colorimetic assay. Demineralized dentin beams were treated with the same five concentrations of PVPA to collagen and then aged for 30 days in a calcium- and zinc-containing medium. The changes in modulus of elasticity, loss of dry mass and dissolution of collagen peptides were measured via three-point bending, precision weighing and hydroxyproline assay, respectively. All tested PVPA concentrations were highly effective (P < 0.05) in inhibiting MMP-9. Ageing in the incubation medium did not significantly alter the modulus of elasticity of the five PVPA treatment groups. Conversely, aged dentin beams from the control group exhibited a significant decline in their modulus of elasticity (P < 0.05) over time. Mass loss from the dentin beams and the corresponding increase in hydroxyproline in the medium in the five PVPA treatment groups were significantly lower than for the control (P < 0.05). PVPA is a potent inhibitor of endogenous MMP activities in demineralized dentin. It may be used as an alternative to chlorhexidine to prevent collagen degradation within hybrid layers to extend the longevity of resin-dentin bonds.

KW - Acid etch

KW - Dentin

KW - Hydroxyproline

KW - Inhibitor

KW - Matrix metalloproteinase

UR - http://www.scopus.com/inward/record.url?scp=77956187959&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77956187959&partnerID=8YFLogxK

U2 - 10.1016/j.actbio.2010.05.017

DO - 10.1016/j.actbio.2010.05.017

M3 - Article

VL - 6

SP - 4136

EP - 4142

JO - Acta Biomaterialia

JF - Acta Biomaterialia

SN - 1742-7061

IS - 10

ER -