TY - JOUR
T1 - The JUUL E-Cigarette Elevates the Risk of Thrombosis and Potentiates Platelet Activation
AU - Ramirez, Jean E.Montes
AU - Karim, Zubair A.
AU - Alarabi, Ahmed B.
AU - Hernandez, Keziah R.
AU - Taleb, Ziyad Ben
AU - Rivera, José O.
AU - Khasawneh, Fadi T.
AU - Alshbool, Fatima Z.
N1 - Funding Information:
The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: Research reported in this publication was supported by the National Institute of Environmental Health Sciences and the National Heart, Lung, and Blood Institute, of the National Institutes of Health under Awards Number R21ES029345 and R01HL145053. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Our studies were also funded using startup funds from The University of Texas at El Paso’s School of Pharmacy (to Alshbool).
Funding Information:
The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: Research reported in this publication was supported by the National Institute of Environmental Health Sciences and the National Heart, Lung, and Blood Institute, of the National Institutes of Health under Awards Number R21ES029345 and R01HL145053. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Our studies were also funded using startup funds from The University of Texas at El Paso?s School of Pharmacy (to Alshbool).
Publisher Copyright:
© The Author(s) 2020.
PY - 2020/11/1
Y1 - 2020/11/1
N2 - Background: Smoking is the main preventable cause of death in the United States and worldwide and is associated with serious cardiovascular health consequences, including thrombotic diseases. Recently, electronic cigarettes (e-cigarettes) and, in particular JUUL, have attained wide popularity among smokers, nonsmokers, pregnant females, and even the youth, which is alarming. Interestingly, there is/are no information/studies regarding the effect of JUUL on cardiovascular diseases, specifically in the context of modulation of platelet activation. Thus, it is important to discern the cardiovascular disease health risks associated with JUUL. Methods and Results: We used a passive e-vape vapor inhalation system where C57BL/6J mice (10-12 weeks old) were exposed to JUUL e-cigarette vape. Menthol flavored JUUL pods containing 5% nicotine by weight were used as the e-liquid. Mice were exposed to a total of 70 puffs daily for 2 weeks; 3-second puff duration, and 25-second puff interval. The effects of JUUL relative to clean air were analyzed, on mouse platelet function in vitro (eg, aggregation) and in vivo (eg, FeCl3-induced carotid artery injury thrombosis model). Our results indicate that short-term exposure to JUUL e-cigarette causes hyperactivation of platelets and shortens the thrombus occlusion as well as hemostasis/bleeding times, relative to clean air (medians of 14 vs. 200 seconds, P <.01 and 35 vs. 295 seconds, P <.001, respectively). Conclusion: Our findings document—for the first time—that short-term exposure to the JUUL e-cigarette increases the risk of thrombotic events, in part by modulating platelet function, such as aggregation and secretion, in mice.
AB - Background: Smoking is the main preventable cause of death in the United States and worldwide and is associated with serious cardiovascular health consequences, including thrombotic diseases. Recently, electronic cigarettes (e-cigarettes) and, in particular JUUL, have attained wide popularity among smokers, nonsmokers, pregnant females, and even the youth, which is alarming. Interestingly, there is/are no information/studies regarding the effect of JUUL on cardiovascular diseases, specifically in the context of modulation of platelet activation. Thus, it is important to discern the cardiovascular disease health risks associated with JUUL. Methods and Results: We used a passive e-vape vapor inhalation system where C57BL/6J mice (10-12 weeks old) were exposed to JUUL e-cigarette vape. Menthol flavored JUUL pods containing 5% nicotine by weight were used as the e-liquid. Mice were exposed to a total of 70 puffs daily for 2 weeks; 3-second puff duration, and 25-second puff interval. The effects of JUUL relative to clean air were analyzed, on mouse platelet function in vitro (eg, aggregation) and in vivo (eg, FeCl3-induced carotid artery injury thrombosis model). Our results indicate that short-term exposure to JUUL e-cigarette causes hyperactivation of platelets and shortens the thrombus occlusion as well as hemostasis/bleeding times, relative to clean air (medians of 14 vs. 200 seconds, P <.01 and 35 vs. 295 seconds, P <.001, respectively). Conclusion: Our findings document—for the first time—that short-term exposure to the JUUL e-cigarette increases the risk of thrombotic events, in part by modulating platelet function, such as aggregation and secretion, in mice.
KW - JUUL
KW - cardiovascular disease
KW - e-cigarettes
KW - platelets
KW - thrombosis
KW - vaping
UR - http://www.scopus.com/inward/record.url?scp=85088305583&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85088305583&partnerID=8YFLogxK
U2 - 10.1177/1074248420941681
DO - 10.1177/1074248420941681
M3 - Article
C2 - 32691614
AN - SCOPUS:85088305583
SN - 1074-2484
VL - 25
SP - 578
EP - 586
JO - Journal of Cardiovascular Pharmacology and Therapeutics
JF - Journal of Cardiovascular Pharmacology and Therapeutics
IS - 6
ER -