The molar ratio of σ73 to core polymerase in the obligate intracellular bacterium, Rickettsia prowazekii

Hanfei Ding, Herbert H. Winkler

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In the obligate Intracellular parasitic bacterium, Rickettsia prowazekii, the molar ratio of σ73 to core RNA polymerase, that is, the degree of saturation of the core polymerase by the catalytically active sigma factor, was very low. This ratio was determined from the radioactivity in rickettsial RNA polymerase immuno‐precipitated from crude extracts of infected L929 cells in which the parasite was exponentially growing. If we assume that, as Is true for the σ subunit, in R. prowazekii and Escherichia coli the β’and β subunits of the RNA polymerase have similar methionine and cysteine contents (the radiolabelled amino acids), the molar ratio of σ73 to core polymerase in R prowazekii would be 0.1. This is in striking contrast to E. Coli where the ratio is typically 0.4. it remains to be established whether this low sigma saturation results In a limitation of active RNA polymerase in R. prowazekii and contributes to its slow growth.

Original languageEnglish (US)
Pages (from-to)869-873
Number of pages5
JournalMolecular Microbiology
Volume11
Issue number5
DOIs
StatePublished - Jan 1 1994
Externally publishedYes

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Rickettsia prowazekii
DNA-Directed RNA Polymerases
Bacteria
Escherichia coli
Sigma Factor
Complex Mixtures
Methionine
Radioactivity
Cysteine
Parasites
Amino Acids
Growth

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

The molar ratio of σ73 to core polymerase in the obligate intracellular bacterium, Rickettsia prowazekii. / Ding, Hanfei; Winkler, Herbert H.

In: Molecular Microbiology, Vol. 11, No. 5, 01.01.1994, p. 869-873.

Research output: Contribution to journalArticle

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