The molluscan cardioactive neuropeptide FMRFamide

Subcellular localization in bivalve ganglia

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Ganglia of the marine mollusk Macrocallista nimbosa were pooled, homogenized, and subjected to differential centrifugation. The neuropeptide Phe‐Met‐Arg‐Phe‐NH2 (FMRFamide) was concentrated in the microsomal pellet. When the medium‐speed supernatant was centrifuged in a discontinuous sucrose gradient, three separate peaks of activity were detected and identified as acetylcholine, 5‐hydroxytryptamine, and FMRFamide. The relative concentration of FMRFamide in each fraction was determined by bioassay and by radioimmunoassay (RIA). Both determinations revealed a peak of peptide in the middle of the sucrose gradient. Electron micrographs of each of the gradient interfaces were analyzed. The interface containing the peak of biological FMRFamide activity was enriched two‐ to fivefold in neurosecretory granules with a mean diameter of 104 nm and various electron densities. Morphologically similar vesicles were also seen in intact ganglia. These findings support the notion that FMRFamide is a neurosecretory product. But the physiological function of the peptide in bivalve ganglia remains unknown.

Original languageEnglish (US)
Pages (from-to)599-611
Number of pages13
JournalJournal of Neurobiology
Volume12
Issue number6
DOIs
StatePublished - Jan 1 1981

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FMRFamide
Bivalvia
Neuropeptides
Ganglia
Sucrose
Electrons
Peptides
Mollusca
Centrifugation
Biological Assay
Acetylcholine
Radioimmunoassay

ASJC Scopus subject areas

  • Neuroscience(all)
  • Cellular and Molecular Neuroscience

Cite this

The molluscan cardioactive neuropeptide FMRFamide : Subcellular localization in bivalve ganglia. / Nagle, Gregg Thomas.

In: Journal of Neurobiology, Vol. 12, No. 6, 01.01.1981, p. 599-611.

Research output: Contribution to journalArticle

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