The molybdenum-pterin binding protein is encoded by a multigene family in clostridium pasteurianum

Stephen M. Hinton, Clive A. Slaughter, William Eisner, Thomas Fisher

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

There are three variants of the molybdenum-pterin binding protein (Mop) encoded by three distinct genes in Clostridium pasteurianum. Nucleotide sequence analysis shows that the three mop genes have greater than 90% homology at the nucleotide level. Upstream from the coding region of each mop gene are potential promoter consensus sequences. Analysis of Mop purified from cells grown under nitrogen-fixing conditions indicates all three genes are expressed. Sequence analysis of the three mop genes and the gene products predicts that there are 10 amino acid replacements among the family. The amino acid replacements are chemically conservative accounting for the co-purification of the three variants of Mop. Protein chemistry data suggest the possibility that glutamic acid residues in Mop may be modified in vivo.

Original languageEnglish (US)
Pages (from-to)211-219
Number of pages9
JournalGene
Volume54
Issue number2-3
DOIs
StatePublished - Jan 1 1987
Externally publishedYes

Fingerprint

Pterins
Molybdenum
Clostridium
Multigene Family
Carrier Proteins
Genes
Sequence Analysis
Amino Acids
Consensus Sequence
Glutamic Acid
Nitrogen
Nucleotides

Keywords

  • Recombinant DNA
  • amino acid replacements
  • glutamic acid derivative
  • nucleotide sequence
  • promoters

ASJC Scopus subject areas

  • Genetics

Cite this

The molybdenum-pterin binding protein is encoded by a multigene family in clostridium pasteurianum. / Hinton, Stephen M.; Slaughter, Clive A.; Eisner, William; Fisher, Thomas.

In: Gene, Vol. 54, No. 2-3, 01.01.1987, p. 211-219.

Research output: Contribution to journalArticle

Hinton, Stephen M. ; Slaughter, Clive A. ; Eisner, William ; Fisher, Thomas. / The molybdenum-pterin binding protein is encoded by a multigene family in clostridium pasteurianum. In: Gene. 1987 ; Vol. 54, No. 2-3. pp. 211-219.
@article{92bb9078c6a741e48ca8c3371298b940,
title = "The molybdenum-pterin binding protein is encoded by a multigene family in clostridium pasteurianum",
abstract = "There are three variants of the molybdenum-pterin binding protein (Mop) encoded by three distinct genes in Clostridium pasteurianum. Nucleotide sequence analysis shows that the three mop genes have greater than 90{\%} homology at the nucleotide level. Upstream from the coding region of each mop gene are potential promoter consensus sequences. Analysis of Mop purified from cells grown under nitrogen-fixing conditions indicates all three genes are expressed. Sequence analysis of the three mop genes and the gene products predicts that there are 10 amino acid replacements among the family. The amino acid replacements are chemically conservative accounting for the co-purification of the three variants of Mop. Protein chemistry data suggest the possibility that glutamic acid residues in Mop may be modified in vivo.",
keywords = "Recombinant DNA, amino acid replacements, glutamic acid derivative, nucleotide sequence, promoters",
author = "Hinton, {Stephen M.} and Slaughter, {Clive A.} and William Eisner and Thomas Fisher",
year = "1987",
month = "1",
day = "1",
doi = "10.1016/0378-1119(87)90489-6",
language = "English (US)",
volume = "54",
pages = "211--219",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "2-3",

}

TY - JOUR

T1 - The molybdenum-pterin binding protein is encoded by a multigene family in clostridium pasteurianum

AU - Hinton, Stephen M.

AU - Slaughter, Clive A.

AU - Eisner, William

AU - Fisher, Thomas

PY - 1987/1/1

Y1 - 1987/1/1

N2 - There are three variants of the molybdenum-pterin binding protein (Mop) encoded by three distinct genes in Clostridium pasteurianum. Nucleotide sequence analysis shows that the three mop genes have greater than 90% homology at the nucleotide level. Upstream from the coding region of each mop gene are potential promoter consensus sequences. Analysis of Mop purified from cells grown under nitrogen-fixing conditions indicates all three genes are expressed. Sequence analysis of the three mop genes and the gene products predicts that there are 10 amino acid replacements among the family. The amino acid replacements are chemically conservative accounting for the co-purification of the three variants of Mop. Protein chemistry data suggest the possibility that glutamic acid residues in Mop may be modified in vivo.

AB - There are three variants of the molybdenum-pterin binding protein (Mop) encoded by three distinct genes in Clostridium pasteurianum. Nucleotide sequence analysis shows that the three mop genes have greater than 90% homology at the nucleotide level. Upstream from the coding region of each mop gene are potential promoter consensus sequences. Analysis of Mop purified from cells grown under nitrogen-fixing conditions indicates all three genes are expressed. Sequence analysis of the three mop genes and the gene products predicts that there are 10 amino acid replacements among the family. The amino acid replacements are chemically conservative accounting for the co-purification of the three variants of Mop. Protein chemistry data suggest the possibility that glutamic acid residues in Mop may be modified in vivo.

KW - Recombinant DNA

KW - amino acid replacements

KW - glutamic acid derivative

KW - nucleotide sequence

KW - promoters

UR - http://www.scopus.com/inward/record.url?scp=0023237351&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023237351&partnerID=8YFLogxK

U2 - 10.1016/0378-1119(87)90489-6

DO - 10.1016/0378-1119(87)90489-6

M3 - Article

VL - 54

SP - 211

EP - 219

JO - Gene

JF - Gene

SN - 0378-1119

IS - 2-3

ER -