The prometastatic ribosomal S6 kinase 2-cAMP response element-binding protein (RSK2-CREB) signaling pathway up-regulates the actin-binding protein fascin-1 to promote tumor metastasis

Dan Li, Lingtao Jin, Gina N. Alesi, Young Mee Kim, Jun Fan, Jae Ho Seo, Dongsheng Wang, Meghan Tucker, Ting Lei Gu, Benjamin H. Lee, Jack Taunton, Kelly R. Magliocca, Zhuo G. Chen, Dong M. Shin, Fadlo R. Khuri, Sumin Kang

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Metastasis is the leading cause of death in patients with breast, lung, and head and neck cancers. However, the molecular mechanisms underlying metastases in these cancers remain unclear. We found that the p90 ribosomal S6 kinase 2 (RSK2)- cAMP response element-binding protein (CREB) pathway is commonly activated in diverse metastatic human cancer cells, leading to up-regulation of a CREB transcription target Fascin- 1. We also observed that the protein expression patterns of RSK2 and Fascin-1 correlate in primary human tumor tissue samples from head and neck squamous cell carcinoma patients. Moreover, knockdown of RSK2 disrupts filopodia formation and bundling in highly invasive cancer cells, leading to attenuated cancer cell invasion in vitro and tumor metastasis in vivo, whereas expression of Fascin-1 significantly rescues these phenotypes. Furthermore, targeting RSK2 with the small molecule RSK inhibitor FMK-MEA effectively attenuated the invasive and metastatic potential of cancer cells in vitro and in vivo, respectively. Taken together, our findings for the first time link RSK2-CREB signaling to filopodia formation and bundling through the up-regulation of Fascin-1, providing a proinvasive and prometastatic advantage to human cancers. Therefore, protein effectors of the RSK2-CREB-Fascin-1 pathway represent promising biomarkers and therapeutic targets in the clinical prognosis and treatment of metastatic human cancers.

Original languageEnglish (US)
Pages (from-to)32528-32538
Number of pages11
JournalJournal of Biological Chemistry
Volume288
Issue number45
DOIs
StatePublished - Nov 8 2013
Externally publishedYes

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Microfilament Proteins
Cyclic AMP Response Element-Binding Protein
Tumors
Up-Regulation
Neoplasm Metastasis
Cells
Neoplasms
Pseudopodia
90-kDa Ribosomal Protein S6 Kinases
Biomarkers
Transcription
fascin
ribosomal protein S6 kinase, 90kDa, polypeptide 3
Proteins
Tissue
Head and Neck Neoplasms
Molecules
Cause of Death
Lung Neoplasms
Breast

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

The prometastatic ribosomal S6 kinase 2-cAMP response element-binding protein (RSK2-CREB) signaling pathway up-regulates the actin-binding protein fascin-1 to promote tumor metastasis. / Li, Dan; Jin, Lingtao; Alesi, Gina N.; Kim, Young Mee; Fan, Jun; Seo, Jae Ho; Wang, Dongsheng; Tucker, Meghan; Gu, Ting Lei; Lee, Benjamin H.; Taunton, Jack; Magliocca, Kelly R.; Chen, Zhuo G.; Shin, Dong M.; Khuri, Fadlo R.; Kang, Sumin.

In: Journal of Biological Chemistry, Vol. 288, No. 45, 08.11.2013, p. 32528-32538.

Research output: Contribution to journalArticle

Li, D, Jin, L, Alesi, GN, Kim, YM, Fan, J, Seo, JH, Wang, D, Tucker, M, Gu, TL, Lee, BH, Taunton, J, Magliocca, KR, Chen, ZG, Shin, DM, Khuri, FR & Kang, S 2013, 'The prometastatic ribosomal S6 kinase 2-cAMP response element-binding protein (RSK2-CREB) signaling pathway up-regulates the actin-binding protein fascin-1 to promote tumor metastasis', Journal of Biological Chemistry, vol. 288, no. 45, pp. 32528-32538. https://doi.org/10.1074/jbc.M113.500561
Li, Dan ; Jin, Lingtao ; Alesi, Gina N. ; Kim, Young Mee ; Fan, Jun ; Seo, Jae Ho ; Wang, Dongsheng ; Tucker, Meghan ; Gu, Ting Lei ; Lee, Benjamin H. ; Taunton, Jack ; Magliocca, Kelly R. ; Chen, Zhuo G. ; Shin, Dong M. ; Khuri, Fadlo R. ; Kang, Sumin. / The prometastatic ribosomal S6 kinase 2-cAMP response element-binding protein (RSK2-CREB) signaling pathway up-regulates the actin-binding protein fascin-1 to promote tumor metastasis. In: Journal of Biological Chemistry. 2013 ; Vol. 288, No. 45. pp. 32528-32538.
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abstract = "Metastasis is the leading cause of death in patients with breast, lung, and head and neck cancers. However, the molecular mechanisms underlying metastases in these cancers remain unclear. We found that the p90 ribosomal S6 kinase 2 (RSK2)- cAMP response element-binding protein (CREB) pathway is commonly activated in diverse metastatic human cancer cells, leading to up-regulation of a CREB transcription target Fascin- 1. We also observed that the protein expression patterns of RSK2 and Fascin-1 correlate in primary human tumor tissue samples from head and neck squamous cell carcinoma patients. Moreover, knockdown of RSK2 disrupts filopodia formation and bundling in highly invasive cancer cells, leading to attenuated cancer cell invasion in vitro and tumor metastasis in vivo, whereas expression of Fascin-1 significantly rescues these phenotypes. Furthermore, targeting RSK2 with the small molecule RSK inhibitor FMK-MEA effectively attenuated the invasive and metastatic potential of cancer cells in vitro and in vivo, respectively. Taken together, our findings for the first time link RSK2-CREB signaling to filopodia formation and bundling through the up-regulation of Fascin-1, providing a proinvasive and prometastatic advantage to human cancers. Therefore, protein effectors of the RSK2-CREB-Fascin-1 pathway represent promising biomarkers and therapeutic targets in the clinical prognosis and treatment of metastatic human cancers.",
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AU - Li, Dan

AU - Jin, Lingtao

AU - Alesi, Gina N.

AU - Kim, Young Mee

AU - Fan, Jun

AU - Seo, Jae Ho

AU - Wang, Dongsheng

AU - Tucker, Meghan

AU - Gu, Ting Lei

AU - Lee, Benjamin H.

AU - Taunton, Jack

AU - Magliocca, Kelly R.

AU - Chen, Zhuo G.

AU - Shin, Dong M.

AU - Khuri, Fadlo R.

AU - Kang, Sumin

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AB - Metastasis is the leading cause of death in patients with breast, lung, and head and neck cancers. However, the molecular mechanisms underlying metastases in these cancers remain unclear. We found that the p90 ribosomal S6 kinase 2 (RSK2)- cAMP response element-binding protein (CREB) pathway is commonly activated in diverse metastatic human cancer cells, leading to up-regulation of a CREB transcription target Fascin- 1. We also observed that the protein expression patterns of RSK2 and Fascin-1 correlate in primary human tumor tissue samples from head and neck squamous cell carcinoma patients. Moreover, knockdown of RSK2 disrupts filopodia formation and bundling in highly invasive cancer cells, leading to attenuated cancer cell invasion in vitro and tumor metastasis in vivo, whereas expression of Fascin-1 significantly rescues these phenotypes. Furthermore, targeting RSK2 with the small molecule RSK inhibitor FMK-MEA effectively attenuated the invasive and metastatic potential of cancer cells in vitro and in vivo, respectively. Taken together, our findings for the first time link RSK2-CREB signaling to filopodia formation and bundling through the up-regulation of Fascin-1, providing a proinvasive and prometastatic advantage to human cancers. Therefore, protein effectors of the RSK2-CREB-Fascin-1 pathway represent promising biomarkers and therapeutic targets in the clinical prognosis and treatment of metastatic human cancers.

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