The role of glutathione in renal cortical tissue. effects of diamide on Na+ and GSSG levels, amino acid transport and Na+-K+-ATPase activity

Dennis J. Pillion, Lamar Moree, Hector Rocha, David Henry Pashley, Joseph Mendicino, Frederick H. Leibach

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The effects of diamide were studied in rat kidney cortical tissue. It was found that diamide increased oxidized glutathione levels and inhibited Na+-K+-ATPase activity. Consistent with this finding was the observation that diamide compromised the sodium gradients maintained in renal cortical slices. Amino acid transport studies with ouabain or a sodium-free buffer indicated that diamide interferes with both Na+-dependent and Na+-independent transport systems. These results indicate that diamide has a number of different effects on renal cortical tissue and emphasize the important role of glutathione in maintaining control of a number of key metabolic pathways.

Original languageEnglish (US)
Pages (from-to)109-115
Number of pages7
JournalMolecular and Cellular Biochemistry
Volume18
Issue number2-3
DOIs
StatePublished - Dec 1 1977

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Diamide
Glutathione Disulfide
Glutathione
Adenosine Triphosphatases
Tissue
Kidney
Amino Acids
Sodium
Ouabain
Metabolic Networks and Pathways
Rats
Buffers
sodium-translocating ATPase

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Clinical Biochemistry
  • Cell Biology

Cite this

The role of glutathione in renal cortical tissue. effects of diamide on Na+ and GSSG levels, amino acid transport and Na+-K+-ATPase activity. / Pillion, Dennis J.; Moree, Lamar; Rocha, Hector; Pashley, David Henry; Mendicino, Joseph; Leibach, Frederick H.

In: Molecular and Cellular Biochemistry, Vol. 18, No. 2-3, 01.12.1977, p. 109-115.

Research output: Contribution to journalArticle

Pillion, Dennis J. ; Moree, Lamar ; Rocha, Hector ; Pashley, David Henry ; Mendicino, Joseph ; Leibach, Frederick H. / The role of glutathione in renal cortical tissue. effects of diamide on Na+ and GSSG levels, amino acid transport and Na+-K+-ATPase activity. In: Molecular and Cellular Biochemistry. 1977 ; Vol. 18, No. 2-3. pp. 109-115.
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