The role of glutathione in renal cortical tissue. effects of diamide on Na+ and GSSG levels, amino acid transport and Na+-K+-ATPase activity

Dennis J. Pillion, Lamar Moree, Hector Rocha, David H. Pashley, Joseph Mendicino, Frederick H. Leibach

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The effects of diamide were studied in rat kidney cortical tissue. It was found that diamide increased oxidized glutathione levels and inhibited Na+-K+-ATPase activity. Consistent with this finding was the observation that diamide compromised the sodium gradients maintained in renal cortical slices. Amino acid transport studies with ouabain or a sodium-free buffer indicated that diamide interferes with both Na+-dependent and Na+-independent transport systems. These results indicate that diamide has a number of different effects on renal cortical tissue and emphasize the important role of glutathione in maintaining control of a number of key metabolic pathways.

Original languageEnglish (US)
Pages (from-to)109-115
Number of pages7
JournalMolecular and Cellular Biochemistry
Issue number2-3
Publication statusPublished - Dec 1 1977


ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

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