The role of mast cells and eosinophils in chronic gastritis

N Moorchung, A N Srivastava, N K Gupta, A K Malaviya, B R Achyut, B Mittal

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Abstract

The role of mast cells and eosinophils in influencing the pathology of chronic gastritis remains unclear. We attempted to study the relationship between endoscopy and the mast cell and eosinophil infiltrate. We also studied the role of gene polymorphisms, Helicobacter pylori density and the CagA antibody status in influencing the mast cell and eosinophil infiltrate. One hundred and twenty consecutive patients were studied. Endoscopic evaluation was done and 3 antral biopsies were taken from each patient and were assessed for eosinophilic and mast cell infiltration, H. pylori density and the density of the other inflammatory cells as per the revised Sydney system. Cytokine gene polymorphisms (IL-1beta, IL-1RA and TNF-alpha) were done on the DNA extracted from the peripheral blood by PCR-RFLP. ELISA was done on the patients' serum for the anti-CagA antibody titres. Nodularity is strongly associated with the presence and density of eosinophils on biopsy (P < 0.05). Eosinophil density is strongly associated with the density of H. pylori, neutrophils, lymphocytes, plasma cells, atrophy, ulceration, foveolitis and lymphoid follicles. The mast cell density is not associated with any of the other histopathological variables. Gene polymorphisms and the CagA antibody titres have no relationship to the mast cell and eosinophil density. Eighty-one patients showed positive anti-CagA antibody titres but there was no association with the eosinophilic or the mast cell infiltrate. It is likely that eosinophilic infiltration is influenced by the H. pylori density but the CagA protein has no role to play in influencing the grade of the eosinophilic infiltrate in the Indian context. Cytokine gene proinflammatory polymorphisms have no role to play in influencing the eosinophilic or the mast cell response. It is likely that other mediators are involved in the inflammatory cell responses.

Original languageEnglish (US)
Pages (from-to)107-14
Number of pages8
JournalClinical and Experimental Medicine
Volume6
Issue number3
DOIs
Publication statusPublished - Oct 2006

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Keywords

  • Adolescent
  • Adult
  • Aged
  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Bacterial Proteins
  • Base Sequence
  • Chronic Disease
  • Cytokines
  • DNA
  • Eosinophils
  • Female
  • Gastritis
  • Helicobacter pylori
  • Humans
  • Male
  • Mast Cells
  • Middle Aged
  • Polymorphism, Genetic
  • Journal Article

Cite this

Moorchung, N., Srivastava, A. N., Gupta, N. K., Malaviya, A. K., Achyut, B. R., & Mittal, B. (2006). The role of mast cells and eosinophils in chronic gastritis. Clinical and Experimental Medicine, 6(3), 107-14. https://doi.org/10.1007/s10238-006-0104-9