The SNARE proteins SNAP25 and synaptobrevin are involved in endocytosis at hippocampal synapses

Zhen Zhang, Dongsheng Wang, Tao Sun, Jianhua Xu, Hsueh Cheng Chiang, Wonchul Shin, Ling Gang Wu

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

SNAP25, an essential component of the soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein receptor (SNARE) complex that mediates exocytosis, is not considered to play a role in endocytosis, which couples to exocytosis by retrieving a similar amount of exocytosed vesicles. By knocking down SNAP25 and imaging slow endocytosis at a conventional synapse, the rat cultured hippocampal synapse, we found that SNAP25 is involved in slow, clathrin-dependent endocytosis. With similar techniques, we found that not only SNAP25, but also synaptobrevin is involved in slow endocytosis. These results provide the first evidence showing the dual role of SNAP25 and synaptobrevin in both exocytosis and slow endocytosis at conventional synapses. Such a dual role may contribute to mediate the coupling between exocytosis and clathrin-dependent endocytosis at conventional synapses, a mechanism critical for the maintenance of synaptic transmission and the normal structure of nerve terminals.

Original languageEnglish (US)
Pages (from-to)9169-9175
Number of pages7
JournalJournal of Neuroscience
Volume33
Issue number21
DOIs
StatePublished - May 22 2013

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N-Ethylmaleimide-Sensitive Proteins
Synaptosomal-Associated Protein 25
R-SNARE Proteins
Endocytosis
Synapses
Exocytosis
Clathrin
Proteins
SNARE Proteins
Synaptic Transmission
Maintenance

ASJC Scopus subject areas

  • Neuroscience(all)

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The SNARE proteins SNAP25 and synaptobrevin are involved in endocytosis at hippocampal synapses. / Zhang, Zhen; Wang, Dongsheng; Sun, Tao; Xu, Jianhua; Chiang, Hsueh Cheng; Shin, Wonchul; Wu, Ling Gang.

In: Journal of Neuroscience, Vol. 33, No. 21, 22.05.2013, p. 9169-9175.

Research output: Contribution to journalArticle

Zhang, Zhen ; Wang, Dongsheng ; Sun, Tao ; Xu, Jianhua ; Chiang, Hsueh Cheng ; Shin, Wonchul ; Wu, Ling Gang. / The SNARE proteins SNAP25 and synaptobrevin are involved in endocytosis at hippocampal synapses. In: Journal of Neuroscience. 2013 ; Vol. 33, No. 21. pp. 9169-9175.
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AU - Shin, Wonchul

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