THE TIME‐RESOLVED PHOTON‐COUNTING FLUOROMETER AT THE NATIONAL SYNCHROTRON LIGHT.SOURCE

William R. Laws, John Clark Sutherland

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A single‐photon‐counting fluorometer, capable of steady‐state and time‐dependent measure‐ments, is now operational at port U9B of the National Synchrotron Light Source at Brookhaven National Laboratory. Excitation wavelengths are selected by a prism‐grating double monochromator. Emission energies are chosen by filter(s) or by a one‐meter Spex monochromator. Commercial electronics are used for data acquisition, except for special circuitry required by the high (53 MHz) repetition rate of the synchrotron. A large sample chamber allows flexibility in arranging optical components and samples. The standard optical configuration of the sample chamber consists of a focusing mirror, a six position excitation optic wheel, a turntable with four thermostatted cuvette holders, a six position emission optic wheel, and an f/0.7 emission collection lens. Data acquisition and the setting of correct wavelengths and sample chamber optics are controlled by a Tektronix 4052 computer via the IEEE‐488 General Purpose Interface Bus. Time‐dependent fluorescence data are transferred to a VAX 11/730 for analysis; current deconvolution software includes nonlinear least‐squares programs for single curve and global analysis.

Original languageEnglish (US)
Pages (from-to)343-348
Number of pages6
JournalPhotochemistry and Photobiology
Volume44
Issue number3
DOIs
StatePublished - Jan 1 1986
Externally publishedYes

Fingerprint

Fluorometers
Synchrotrons
Light sources
Optics
synchrotrons
Monochromators
Light
Optical Devices
chambers
Data acquisition
Wheels
optics
monochromators
wheels
Lenses
data acquisition
Phonographs
Wavelength
Software
Fluorescence

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry

Cite this

THE TIME‐RESOLVED PHOTON‐COUNTING FLUOROMETER AT THE NATIONAL SYNCHROTRON LIGHT.SOURCE. / Laws, William R.; Sutherland, John Clark.

In: Photochemistry and Photobiology, Vol. 44, No. 3, 01.01.1986, p. 343-348.

Research output: Contribution to journalArticle

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